Strain Fitness in Escherichia coli BW25113 around b3230

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsspA and rpsI are separated by 394 nucleotidesrpsI and rplM are separated by 15 nucleotidesrplM and yhcM are separated by 218 nucleotides b3229: sspA - stringent starvation protein A (NCBI), at 3,374,804 to 3,375,442 sspA b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229 rpsI b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673 rplM b3232: yhcM - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,376,892 to 3,378,019 yhcM Position (kb) 3375 3376 3377Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3374.966 kb on - strand, within sspAat 3374.976 kb on + strand, within sspAat 3374.976 kb on + strand, within sspAat 3375.071 kb on - strand, within sspAat 3375.073 kb on - strand, within sspAat 3375.084 kb on - strand, within sspAat 3375.098 kb on + strand, within sspAat 3375.098 kb on + strand, within sspAat 3375.113 kb on - strand, within sspAat 3375.189 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.220 kb on - strand, within sspAat 3375.236 kb on - strand, within sspAat 3375.353 kb on + strand, within sspAat 3375.353 kb on + strand, within sspAat 3375.515 kb on - strandat 3376.719 kb on - strandat 3376.719 kb on - strandat 3376.844 kb on - strandat 3376.845 kb on - strandat 3376.929 kb on - strandat 3377.108 kb on + strand, within yhcMat 3377.108 kb on + strand, within yhcMat 3377.130 kb on - strand, within yhcMat 3377.162 kb on - strand, within yhcMat 3377.182 kb on - strand, within yhcMat 3377.191 kb on + strand, within yhcMat 3377.191 kb on + strand, within yhcMat 3377.193 kb on + strand, within yhcMat 3377.193 kb on + strand, within yhcM

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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3,374,966 - sspA b3229 0.25 +5.0
3,374,976 + sspA b3229 0.27 +4.3
3,374,976 + sspA b3229 0.27 +4.5
3,375,071 - sspA b3229 0.42 +4.1
3,375,073 - sspA b3229 0.42 +4.5
3,375,084 - sspA b3229 0.44 +4.1
3,375,098 + sspA b3229 0.46 +3.9
3,375,098 + sspA b3229 0.46 +4.2
3,375,113 - sspA b3229 0.48 +4.8
3,375,189 - sspA b3229 0.60 +3.8
3,375,220 - sspA b3229 0.65 +4.9
3,375,220 - sspA b3229 0.65 +4.3
3,375,220 - sspA b3229 0.65 +4.3
3,375,236 - sspA b3229 0.68 +4.7
3,375,353 + sspA b3229 0.86 +4.4
3,375,353 + sspA b3229 0.86 +4.9
3,375,515 - +1.6
3,376,719 - -1.6
3,376,719 - -1.1
3,376,844 - -0.3
3,376,845 - -1.5
3,376,929 - -1.6
3,377,108 + yhcM b3232 0.19 -3.2
3,377,108 + yhcM b3232 0.19 -2.3
3,377,130 - yhcM b3232 0.21 -1.7
3,377,162 - yhcM b3232 0.24 -2.6
3,377,182 - yhcM b3232 0.26 -2.0
3,377,191 + yhcM b3232 0.27 -1.6
3,377,191 + yhcM b3232 0.27 -3.1
3,377,193 + yhcM b3232 0.27 -1.4
3,377,193 + yhcM b3232 0.27 -3.7

Or see this region's nucleotide sequence