Strain Fitness in Escherichia coli BW25113 around b2538

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaR and hcaE are separated by 135 nucleotideshcaE and hcaF overlap by 4 nucleotideshcaF and hcaC overlap by 1 nucleotideshcaC and hcaB overlap by 4 nucleotides b2537: hcaR - DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI), at 2,666,028 to 2,666,918 hcaR b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415 hcaE b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930 hcaF b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250 hcaC b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059 hcaB Position (kb) 2667 2668 2669Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2666.111 kb on - strandat 2666.203 kb on - strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.216 kb on - strand, within hcaRat 2666.269 kb on - strand, within hcaRat 2666.334 kb on - strand, within hcaRat 2666.359 kb on + strand, within hcaRat 2666.361 kb on + strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.551 kb on + strand, within hcaRat 2666.563 kb on + strand, within hcaRat 2666.824 kb on + strand, within hcaRat 2666.839 kb on - strandat 2666.877 kb on + strandat 2666.877 kb on + strandat 2666.931 kb on - strandat 2667.028 kb on - strandat 2667.028 kb on - strandat 2667.079 kb on + strandat 2667.079 kb on + strandat 2667.364 kb on - strand, within hcaEat 2667.364 kb on - strand, within hcaEat 2667.536 kb on + strand, within hcaEat 2667.646 kb on - strand, within hcaEat 2667.730 kb on - strand, within hcaEat 2667.827 kb on - strand, within hcaEat 2667.880 kb on - strand, within hcaEat 2667.930 kb on - strand, within hcaEat 2667.956 kb on + strand, within hcaEat 2668.118 kb on - strand, within hcaEat 2668.174 kb on - strand, within hcaEat 2668.253 kb on + strand, within hcaEat 2668.253 kb on + strand, within hcaEat 2668.363 kb on + strandat 2668.539 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.709 kb on + strand, within hcaFat 2668.715 kb on + strand, within hcaFat 2668.736 kb on - strand, within hcaFat 2668.759 kb on + strand, within hcaFat 2668.888 kb on - strandat 2668.888 kb on - strandat 2668.925 kb on - strandat 2668.932 kb on - strandat 2668.979 kb on - strand, within hcaCat 2669.083 kb on + strand, within hcaCat 2669.112 kb on - strand, within hcaCat 2669.166 kb on + strand, within hcaCat 2669.295 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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2,666,111 - +0.3
2,666,203 - hcaR b2537 0.20 -0.4
2,666,213 + hcaR b2537 0.21 +0.5
2,666,213 + hcaR b2537 0.21 +1.0
2,666,216 - hcaR b2537 0.21 -3.1
2,666,269 - hcaR b2537 0.27 +0.1
2,666,334 - hcaR b2537 0.34 +0.7
2,666,359 + hcaR b2537 0.37 +0.3
2,666,361 + hcaR b2537 0.37 -0.0
2,666,389 - hcaR b2537 0.41 +0.4
2,666,389 - hcaR b2537 0.41 +0.2
2,666,551 + hcaR b2537 0.59 +0.5
2,666,563 + hcaR b2537 0.60 +0.2
2,666,824 + hcaR b2537 0.89 +0.4
2,666,839 - -0.2
2,666,877 + +0.1
2,666,877 + +0.6
2,666,931 - +0.2
2,667,028 - -0.1
2,667,028 - +0.1
2,667,079 + +0.3
2,667,079 + +0.8
2,667,364 - hcaE b2538 0.23 +0.7
2,667,364 - hcaE b2538 0.23 +0.4
2,667,536 + hcaE b2538 0.35 +0.3
2,667,646 - hcaE b2538 0.43 -0.1
2,667,730 - hcaE b2538 0.50 +0.5
2,667,827 - hcaE b2538 0.57 +0.2
2,667,880 - hcaE b2538 0.61 -1.2
2,667,930 - hcaE b2538 0.64 -1.1
2,667,956 + hcaE b2538 0.66 +0.2
2,668,118 - hcaE b2538 0.78 +0.8
2,668,174 - hcaE b2538 0.82 +0.3
2,668,253 + hcaE b2538 0.88 +2.0
2,668,253 + hcaE b2538 0.88 +1.0
2,668,363 + +0.7
2,668,539 - hcaF b2539 0.24 +0.2
2,668,551 - hcaF b2539 0.27 -0.9
2,668,551 - hcaF b2539 0.27 +0.8
2,668,676 + hcaF b2539 0.51 +0.5
2,668,676 + hcaF b2539 0.51 -0.5
2,668,709 + hcaF b2539 0.57 -0.6
2,668,715 + hcaF b2539 0.58 +0.8
2,668,736 - hcaF b2539 0.62 +0.0
2,668,759 + hcaF b2539 0.67 +0.6
2,668,888 - -1.7
2,668,888 - -0.3
2,668,925 - -0.8
2,668,932 - +0.2
2,668,979 - hcaC b2540 0.15 -0.9
2,669,083 + hcaC b2540 0.48 -1.4
2,669,112 - hcaC b2540 0.57 -0.4
2,669,166 + hcaC b2540 0.74 +0.3
2,669,295 + -0.1

Or see this region's nucleotide sequence