Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaR and hcaE are separated by 135 nucleotides hcaE and hcaF overlap by 4 nucleotides hcaF and hcaC overlap by 1 nucleotides hcaC and hcaB overlap by 4 nucleotides
b2537: hcaR - DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI), at 2,666,028 to 2,666,918
hcaR
b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415
hcaE
b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930
hcaF
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
Position (kb)
2667
2668
2669 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2666.111 kb on - strand at 2666.203 kb on - strand, within hcaR at 2666.213 kb on + strand, within hcaR at 2666.213 kb on + strand, within hcaR at 2666.216 kb on - strand, within hcaR at 2666.269 kb on - strand, within hcaR at 2666.334 kb on - strand, within hcaR at 2666.359 kb on + strand, within hcaR at 2666.361 kb on + strand, within hcaR at 2666.389 kb on - strand, within hcaR at 2666.389 kb on - strand, within hcaR at 2666.551 kb on + strand, within hcaR at 2666.563 kb on + strand, within hcaR at 2666.824 kb on + strand, within hcaR at 2666.839 kb on - strand at 2666.877 kb on + strand at 2666.877 kb on + strand at 2666.931 kb on - strand at 2667.028 kb on - strand at 2667.028 kb on - strand at 2667.079 kb on + strand at 2667.079 kb on + strand at 2667.364 kb on - strand, within hcaE at 2667.364 kb on - strand, within hcaE at 2667.536 kb on + strand, within hcaE at 2667.646 kb on - strand, within hcaE at 2667.730 kb on - strand, within hcaE at 2667.827 kb on - strand, within hcaE at 2667.880 kb on - strand, within hcaE at 2667.930 kb on - strand, within hcaE at 2667.956 kb on + strand, within hcaE at 2668.118 kb on - strand, within hcaE at 2668.174 kb on - strand, within hcaE at 2668.253 kb on + strand, within hcaE at 2668.253 kb on + strand, within hcaE at 2668.363 kb on + strand at 2668.539 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.551 kb on - strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.676 kb on + strand, within hcaF at 2668.709 kb on + strand, within hcaF at 2668.715 kb on + strand, within hcaF at 2668.736 kb on - strand, within hcaF at 2668.759 kb on + strand, within hcaF at 2668.888 kb on - strand at 2668.888 kb on - strand at 2668.925 kb on - strand at 2668.932 kb on - strand at 2668.979 kb on - strand, within hcaC at 2669.083 kb on + strand, within hcaC at 2669.112 kb on - strand, within hcaC at 2669.166 kb on + strand, within hcaC at 2669.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 2,666,111 - +0.3 2,666,203 - hcaR b2537 0.20 -0.4 2,666,213 + hcaR b2537 0.21 +0.5 2,666,213 + hcaR b2537 0.21 +1.0 2,666,216 - hcaR b2537 0.21 -3.1 2,666,269 - hcaR b2537 0.27 +0.1 2,666,334 - hcaR b2537 0.34 +0.7 2,666,359 + hcaR b2537 0.37 +0.3 2,666,361 + hcaR b2537 0.37 -0.0 2,666,389 - hcaR b2537 0.41 +0.4 2,666,389 - hcaR b2537 0.41 +0.2 2,666,551 + hcaR b2537 0.59 +0.5 2,666,563 + hcaR b2537 0.60 +0.2 2,666,824 + hcaR b2537 0.89 +0.4 2,666,839 - -0.2 2,666,877 + +0.1 2,666,877 + +0.6 2,666,931 - +0.2 2,667,028 - -0.1 2,667,028 - +0.1 2,667,079 + +0.3 2,667,079 + +0.8 2,667,364 - hcaE b2538 0.23 +0.7 2,667,364 - hcaE b2538 0.23 +0.4 2,667,536 + hcaE b2538 0.35 +0.3 2,667,646 - hcaE b2538 0.43 -0.1 2,667,730 - hcaE b2538 0.50 +0.5 2,667,827 - hcaE b2538 0.57 +0.2 2,667,880 - hcaE b2538 0.61 -1.2 2,667,930 - hcaE b2538 0.64 -1.1 2,667,956 + hcaE b2538 0.66 +0.2 2,668,118 - hcaE b2538 0.78 +0.8 2,668,174 - hcaE b2538 0.82 +0.3 2,668,253 + hcaE b2538 0.88 +2.0 2,668,253 + hcaE b2538 0.88 +1.0 2,668,363 + +0.7 2,668,539 - hcaF b2539 0.24 +0.2 2,668,551 - hcaF b2539 0.27 -0.9 2,668,551 - hcaF b2539 0.27 +0.8 2,668,676 + hcaF b2539 0.51 +0.5 2,668,676 + hcaF b2539 0.51 -0.5 2,668,709 + hcaF b2539 0.57 -0.6 2,668,715 + hcaF b2539 0.58 +0.8 2,668,736 - hcaF b2539 0.62 +0.0 2,668,759 + hcaF b2539 0.67 +0.6 2,668,888 - -1.7 2,668,888 - -0.3 2,668,925 - -0.8 2,668,932 - +0.2 2,668,979 - hcaC b2540 0.15 -0.9 2,669,083 + hcaC b2540 0.48 -1.4 2,669,112 - hcaC b2540 0.57 -0.4 2,669,166 + hcaC b2540 0.74 +0.3 2,669,295 + -0.1
Or see this region's nucleotide sequence