Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4420 and EX28DRAFT_4421 are separated by 9 nucleotides EX28DRAFT_4421 and EX28DRAFT_4422 are separated by 74 nucleotides EX28DRAFT_4422 and EX28DRAFT_4423 are separated by 129 nucleotides EX28DRAFT_4423 and EX28DRAFT_4424 are separated by 96 nucleotides
EX28DRAFT_4420: EX28DRAFT_4420 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 87,709 to 88,713
_4420
EX28DRAFT_4421: EX28DRAFT_4421 - N-acetylglutamate kinase (EC 2.7.2.8), at 88,723 to 89,496
_4421
EX28DRAFT_4422: EX28DRAFT_4422 - argininosuccinate lyase, at 89,571 to 90,944
_4422
EX28DRAFT_4423: EX28DRAFT_4423 - RNA, at 91,074 to 91,184
_4423
EX28DRAFT_4424: EX28DRAFT_4424 - Transcriptional regulator, at 91,281 to 92,198
_4424
Position (kb) 89
90
91 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 88.635 kb on - strand at 88.674 kb on + strand at 88.675 kb on - strand at 88.707 kb on + strand at 88.737 kb on + strand at 88.754 kb on + strand at 89.048 kb on + strand, within EX28DRAFT_4421 at 89.484 kb on + strand at 89.484 kb on + strand at 89.490 kb on - strand at 89.515 kb on + strand at 89.549 kb on + strand at 89.670 kb on - strand at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.738 kb on - strand, within EX28DRAFT_4422 at 89.933 kb on + strand, within EX28DRAFT_4422 at 90.184 kb on - strand, within EX28DRAFT_4422 at 90.521 kb on + strand, within EX28DRAFT_4422 at 90.521 kb on + strand, within EX28DRAFT_4422 at 90.522 kb on - strand, within EX28DRAFT_4422 at 90.706 kb on + strand, within EX28DRAFT_4422 at 90.728 kb on + strand, within EX28DRAFT_4422 at 90.729 kb on - strand, within EX28DRAFT_4422 at 91.184 kb on + strand at 91.193 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 88,635 - +1.0 88,674 + -2.8 88,675 - +1.4 88,707 + -0.8 88,737 + +2.0 88,754 + +1.0 89,048 + EX28DRAFT_4421 0.42 -2.1 89,484 + -0.7 89,484 + -1.3 89,490 - -2.2 89,515 + -1.2 89,549 + +0.3 89,670 - -1.7 89,738 - EX28DRAFT_4422 0.12 -2.4 89,738 - EX28DRAFT_4422 0.12 -1.2 89,933 + EX28DRAFT_4422 0.26 -1.9 90,184 - EX28DRAFT_4422 0.45 -2.6 90,521 + EX28DRAFT_4422 0.69 -1.7 90,521 + EX28DRAFT_4422 0.69 -1.8 90,522 - EX28DRAFT_4422 0.69 -0.8 90,706 + EX28DRAFT_4422 0.83 -3.6 90,728 + EX28DRAFT_4422 0.84 +1.0 90,729 - EX28DRAFT_4422 0.84 -1.3 91,184 + -0.4 91,193 + +1.0
Or see this region's nucleotide sequence