Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4136 and EX28DRAFT_4137 are separated by 88 nucleotides EX28DRAFT_4137 and EX28DRAFT_4138 are separated by 232 nucleotides EX28DRAFT_4138 and EX28DRAFT_4139 overlap by 4 nucleotides
EX28DRAFT_4136: EX28DRAFT_4136 - Transcriptional accessory protein, at 114,617 to 116,947
_4136
EX28DRAFT_4137: EX28DRAFT_4137 - transcription elongation factor GreB, at 117,036 to 117,509
_4137
EX28DRAFT_4138: EX28DRAFT_4138 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 117,742 to 118,461
_4138
EX28DRAFT_4139: EX28DRAFT_4139 - Signal transduction histidine kinase, at 118,458 to 119,804
_4139
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1 at 116.785 kb on + strand at 116.886 kb on - strand at 116.908 kb on + strand at 116.920 kb on + strand at 116.984 kb on + strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 117.042 kb on + strand at 117.043 kb on - strand at 117.064 kb on + strand at 117.065 kb on - strand at 117.065 kb on - strand at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.362 kb on - strand, within EX28DRAFT_4137 at 117.367 kb on + strand, within EX28DRAFT_4137 at 117.535 kb on + strand at 117.553 kb on + strand at 117.553 kb on + strand at 117.582 kb on + strand at 117.583 kb on - strand at 117.593 kb on + strand at 117.593 kb on + strand at 117.614 kb on - strand at 117.625 kb on - strand at 117.638 kb on - strand at 117.668 kb on + strand at 117.669 kb on - strand at 117.681 kb on - strand at 117.700 kb on - strand at 117.700 kb on - strand at 117.809 kb on - strand at 117.936 kb on + strand, within EX28DRAFT_4138 at 118.046 kb on - strand, within EX28DRAFT_4138 at 118.321 kb on + strand, within EX28DRAFT_4138 at 118.402 kb on + strand at 118.402 kb on + strand at 118.404 kb on + strand at 118.404 kb on + strand at 118.405 kb on - strand at 118.405 kb on - strand at 118.405 kb on - strand at 118.484 kb on + strand at 118.549 kb on - strand at 118.549 kb on - strand at 118.595 kb on + strand, within EX28DRAFT_4139 at 118.596 kb on - strand, within EX28DRAFT_4139 at 118.596 kb on - strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.598 kb on + strand, within EX28DRAFT_4139 at 118.599 kb on - strand, within EX28DRAFT_4139 at 118.622 kb on + strand, within EX28DRAFT_4139 at 118.623 kb on - strand, within EX28DRAFT_4139 at 118.987 kb on + strand, within EX28DRAFT_4139 at 118.988 kb on - strand, within EX28DRAFT_4139 at 118.997 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 118.999 kb on + strand, within EX28DRAFT_4139 at 119.000 kb on - strand, within EX28DRAFT_4139 at 119.057 kb on + strand, within EX28DRAFT_4139 at 119.117 kb on + strand, within EX28DRAFT_4139 at 119.123 kb on + strand, within EX28DRAFT_4139 at 119.124 kb on - strand, within EX28DRAFT_4139 at 119.160 kb on - strand, within EX28DRAFT_4139 at 119.211 kb on - strand, within EX28DRAFT_4139 at 119.251 kb on - strand, within EX28DRAFT_4139 at 119.270 kb on + strand, within EX28DRAFT_4139 at 119.289 kb on - strand, within EX28DRAFT_4139 at 119.446 kb on + strand, within EX28DRAFT_4139
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 116,785 + +0.0 116,886 - -0.4 116,908 + +0.5 116,920 + +0.5 116,984 + -0.0 116,985 - +0.1 116,985 - -0.5 116,985 - +0.5 116,989 - +1.1 116,989 - -0.6 116,989 - +0.3 117,042 + -0.6 117,043 - +0.7 117,064 + +0.5 117,065 - -1.8 117,065 - -0.1 117,160 + EX28DRAFT_4137 0.26 +0.4 117,160 + EX28DRAFT_4137 0.26 -0.9 117,161 - EX28DRAFT_4137 0.26 -0.2 117,161 - EX28DRAFT_4137 0.26 -1.2 117,362 - EX28DRAFT_4137 0.69 +0.0 117,367 + EX28DRAFT_4137 0.70 -0.5 117,535 + -0.4 117,553 + +0.4 117,553 + -0.3 117,582 + -0.1 117,583 - -1.7 117,593 + -0.0 117,593 + -0.1 117,614 - -0.8 117,625 - -2.7 117,638 - -0.9 117,668 + +0.7 117,669 - -0.4 117,681 - -0.2 117,700 - +0.8 117,700 - -0.5 117,809 - +0.9 117,936 + EX28DRAFT_4138 0.27 -0.9 118,046 - EX28DRAFT_4138 0.42 -0.8 118,321 + EX28DRAFT_4138 0.80 -1.2 118,402 + -0.7 118,402 + -0.4 118,404 + -0.2 118,404 + +0.2 118,405 - -1.5 118,405 - +0.0 118,405 - -0.1 118,484 + +0.3 118,549 - -0.1 118,549 - -0.2 118,595 + EX28DRAFT_4139 0.10 -0.6 118,596 - EX28DRAFT_4139 0.10 +0.4 118,596 - EX28DRAFT_4139 0.10 -0.0 118,598 + EX28DRAFT_4139 0.10 -0.2 118,598 + EX28DRAFT_4139 0.10 +0.2 118,598 + EX28DRAFT_4139 0.10 +0.0 118,598 + EX28DRAFT_4139 0.10 -0.8 118,599 - EX28DRAFT_4139 0.10 -0.7 118,622 + EX28DRAFT_4139 0.12 +0.2 118,623 - EX28DRAFT_4139 0.12 +0.2 118,987 + EX28DRAFT_4139 0.39 -0.3 118,988 - EX28DRAFT_4139 0.39 -0.3 118,997 + EX28DRAFT_4139 0.40 -1.1 118,999 + EX28DRAFT_4139 0.40 +0.4 118,999 + EX28DRAFT_4139 0.40 +0.0 118,999 + EX28DRAFT_4139 0.40 +0.1 119,000 - EX28DRAFT_4139 0.40 +0.4 119,057 + EX28DRAFT_4139 0.44 +0.3 119,117 + EX28DRAFT_4139 0.49 -0.4 119,123 + EX28DRAFT_4139 0.49 +0.6 119,124 - EX28DRAFT_4139 0.49 +0.0 119,160 - EX28DRAFT_4139 0.52 +0.1 119,211 - EX28DRAFT_4139 0.56 +0.4 119,251 - EX28DRAFT_4139 0.59 -0.5 119,270 + EX28DRAFT_4139 0.60 +0.6 119,289 - EX28DRAFT_4139 0.62 -0.5 119,446 + EX28DRAFT_4139 0.73 +0.5
Or see this region's nucleotide sequence