Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3776 and EX28DRAFT_3777 are separated by 28 nucleotides EX28DRAFT_3777 and EX28DRAFT_3778 overlap by 14 nucleotides EX28DRAFT_3778 and EX28DRAFT_3779 are separated by 196 nucleotides EX28DRAFT_3779 and EX28DRAFT_3780 overlap by 4 nucleotides
EX28DRAFT_3776: EX28DRAFT_3776 - tRNA(Ile)-lysidine synthetase, N-terminal domain/tRNA(Ile)-lysidine synthetase, C-terminal domain, at 459,794 to 461,083
_3776
EX28DRAFT_3777: EX28DRAFT_3777 - transcriptional antiterminator, Rof, at 461,112 to 461,372
_3777
EX28DRAFT_3778: EX28DRAFT_3778 - Uncharacterised protein family (UPF0253), at 461,359 to 461,559
_3778
EX28DRAFT_3779: EX28DRAFT_3779 - Uncharacterized protein conserved in bacteria, at 461,756 to 462,301
_3779
EX28DRAFT_3780: EX28DRAFT_3780 - RF-1 domain, at 462,298 to 462,720
_3780
Position (kb)
461
462 Strain fitness (log2 ratio)
-2
-1
0
1 at 461.164 kb on - strand, within EX28DRAFT_3777 at 461.295 kb on + strand, within EX28DRAFT_3777 at 461.295 kb on + strand, within EX28DRAFT_3777 at 461.296 kb on - strand, within EX28DRAFT_3777 at 461.330 kb on - strand, within EX28DRAFT_3777 at 461.353 kb on + strand at 461.354 kb on - strand at 461.354 kb on - strand at 461.357 kb on + strand at 461.357 kb on + strand at 461.357 kb on + strand at 461.357 kb on + strand at 461.358 kb on - strand at 461.402 kb on + strand, within EX28DRAFT_3778 at 461.457 kb on + strand, within EX28DRAFT_3778 at 461.495 kb on + strand, within EX28DRAFT_3778 at 461.540 kb on - strand at 461.540 kb on - strand at 461.554 kb on - strand at 461.554 kb on - strand at 461.586 kb on - strand at 461.602 kb on - strand at 461.607 kb on + strand at 461.608 kb on - strand at 461.616 kb on - strand at 461.639 kb on + strand at 461.639 kb on - strand at 461.639 kb on - strand at 461.639 kb on - strand at 461.640 kb on + strand at 461.640 kb on + strand at 461.640 kb on + strand at 461.641 kb on - strand at 461.641 kb on - strand at 461.682 kb on + strand at 461.682 kb on + strand at 461.687 kb on - strand at 461.899 kb on + strand, within EX28DRAFT_3779 at 461.899 kb on + strand, within EX28DRAFT_3779 at 461.903 kb on + strand, within EX28DRAFT_3779 at 461.903 kb on + strand, within EX28DRAFT_3779 at 461.903 kb on + strand, within EX28DRAFT_3779 at 461.904 kb on - strand, within EX28DRAFT_3779 at 461.995 kb on - strand, within EX28DRAFT_3779 at 462.032 kb on - strand, within EX28DRAFT_3779 at 462.032 kb on - strand, within EX28DRAFT_3779 at 462.075 kb on - strand, within EX28DRAFT_3779 at 462.448 kb on + strand, within EX28DRAFT_3780 at 462.448 kb on + strand, within EX28DRAFT_3780 at 462.451 kb on + strand, within EX28DRAFT_3780 at 462.451 kb on + strand, within EX28DRAFT_3780 at 462.452 kb on - strand, within EX28DRAFT_3780 at 462.452 kb on - strand, within EX28DRAFT_3780 at 462.452 kb on - strand, within EX28DRAFT_3780 at 462.452 kb on - strand, within EX28DRAFT_3780 at 462.452 kb on - strand, within EX28DRAFT_3780 at 462.453 kb on + strand, within EX28DRAFT_3780
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 461,164 - EX28DRAFT_3777 0.20 -0.4 461,295 + EX28DRAFT_3777 0.70 -2.2 461,295 + EX28DRAFT_3777 0.70 +1.1 461,296 - EX28DRAFT_3777 0.70 -0.1 461,330 - EX28DRAFT_3777 0.84 +0.5 461,353 + -0.0 461,354 - +0.3 461,354 - +0.9 461,357 + +0.8 461,357 + -0.4 461,357 + +0.0 461,357 + +0.1 461,358 - -0.2 461,402 + EX28DRAFT_3778 0.21 +0.4 461,457 + EX28DRAFT_3778 0.49 +1.2 461,495 + EX28DRAFT_3778 0.68 -0.1 461,540 - +0.4 461,540 - +1.1 461,554 - +0.3 461,554 - -0.0 461,586 - -0.1 461,602 - -0.6 461,607 + -1.4 461,608 - +0.0 461,616 - -0.5 461,639 + +0.8 461,639 - +0.5 461,639 - +0.0 461,639 - -0.2 461,640 + +0.0 461,640 + +0.2 461,640 + -0.2 461,641 - -0.9 461,641 - -0.2 461,682 + +0.0 461,682 + -0.1 461,687 - +0.0 461,899 + EX28DRAFT_3779 0.26 +0.5 461,899 + EX28DRAFT_3779 0.26 +0.0 461,903 + EX28DRAFT_3779 0.27 -0.4 461,903 + EX28DRAFT_3779 0.27 +1.6 461,903 + EX28DRAFT_3779 0.27 -0.7 461,904 - EX28DRAFT_3779 0.27 -2.0 461,995 - EX28DRAFT_3779 0.44 -0.2 462,032 - EX28DRAFT_3779 0.51 -0.4 462,032 - EX28DRAFT_3779 0.51 -0.1 462,075 - EX28DRAFT_3779 0.58 -0.0 462,448 + EX28DRAFT_3780 0.35 -0.4 462,448 + EX28DRAFT_3780 0.35 -0.2 462,451 + EX28DRAFT_3780 0.36 -0.0 462,451 + EX28DRAFT_3780 0.36 -0.1 462,452 - EX28DRAFT_3780 0.36 -0.6 462,452 - EX28DRAFT_3780 0.36 -0.4 462,452 - EX28DRAFT_3780 0.36 +0.9 462,452 - EX28DRAFT_3780 0.36 -0.6 462,452 - EX28DRAFT_3780 0.36 -0.3 462,453 + EX28DRAFT_3780 0.37 -0.4
Or see this region's nucleotide sequence