Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3614 and EX28DRAFT_3615 are separated by 1 nucleotides EX28DRAFT_3615 and EX28DRAFT_3616 are separated by 3 nucleotides EX28DRAFT_3616 and EX28DRAFT_3617 are separated by 257 nucleotides EX28DRAFT_3617 and EX28DRAFT_3618 are separated by 32 nucleotides
EX28DRAFT_3614: EX28DRAFT_3614 - aspartate kinase (EC 2.7.2.4), at 272,367 to 274,829
_3614
EX28DRAFT_3615: EX28DRAFT_3615 - homoserine kinase (EC 2.7.1.39), at 274,831 to 275,760
_3615
EX28DRAFT_3616: EX28DRAFT_3616 - threonine synthase, at 275,764 to 277,050
_3616
EX28DRAFT_3617: EX28DRAFT_3617 - Protein of unknown function (DUF2502), at 277,308 to 277,610
_3617
EX28DRAFT_3618: EX28DRAFT_3618 - Uncharacterized protein conserved in bacteria, at 277,643 to 278,416
_3618
Position (kb) 275
276
277
278 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.820 kb on - strand at 275.019 kb on + strand, within EX28DRAFT_3615 at 275.683 kb on - strand at 275.719 kb on - strand at 275.758 kb on + strand at 275.758 kb on + strand at 275.762 kb on + strand at 275.763 kb on - strand at 275.763 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.782 kb on - strand at 275.790 kb on + strand at 276.200 kb on - strand, within EX28DRAFT_3616 at 276.276 kb on + strand, within EX28DRAFT_3616 at 276.437 kb on - strand, within EX28DRAFT_3616 at 276.807 kb on + strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.808 kb on - strand, within EX28DRAFT_3616 at 276.920 kb on + strand, within EX28DRAFT_3616 at 277.087 kb on + strand at 277.112 kb on + strand at 277.200 kb on - strand at 277.267 kb on + strand at 277.313 kb on + strand at 277.393 kb on + strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.394 kb on - strand, within EX28DRAFT_3617 at 277.403 kb on - strand, within EX28DRAFT_3617 at 277.428 kb on - strand, within EX28DRAFT_3617 at 277.488 kb on + strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.489 kb on - strand, within EX28DRAFT_3617 at 277.644 kb on + strand at 277.704 kb on + strand at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.890 kb on + strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.891 kb on - strand, within EX28DRAFT_3618 at 277.893 kb on + strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.894 kb on - strand, within EX28DRAFT_3618 at 277.986 kb on + strand, within EX28DRAFT_3618
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 274,820 - -1.1 275,019 + EX28DRAFT_3615 0.20 -1.4 275,683 - -1.4 275,719 - -0.7 275,758 + -2.7 275,758 + -1.4 275,762 + +0.9 275,763 - -2.0 275,763 - +0.3 275,774 - +0.3 275,774 - +0.3 275,774 - -0.7 275,782 - -1.5 275,790 + -1.4 276,200 - EX28DRAFT_3616 0.34 +1.9 276,276 + EX28DRAFT_3616 0.40 +1.9 276,437 - EX28DRAFT_3616 0.52 -3.4 276,807 + EX28DRAFT_3616 0.81 -1.4 276,808 - EX28DRAFT_3616 0.81 -2.1 276,808 - EX28DRAFT_3616 0.81 -1.2 276,920 + EX28DRAFT_3616 0.90 -0.2 277,087 + -0.2 277,112 + +0.1 277,200 - +0.2 277,267 + -0.1 277,313 + -0.2 277,393 + EX28DRAFT_3617 0.28 -0.2 277,394 - EX28DRAFT_3617 0.28 +0.5 277,394 - EX28DRAFT_3617 0.28 -0.1 277,403 - EX28DRAFT_3617 0.31 -0.1 277,428 - EX28DRAFT_3617 0.40 +0.0 277,488 + EX28DRAFT_3617 0.59 +0.3 277,489 - EX28DRAFT_3617 0.60 -0.1 277,489 - EX28DRAFT_3617 0.60 +0.6 277,644 + -1.1 277,704 + +0.8 277,890 + EX28DRAFT_3618 0.32 -0.6 277,890 + EX28DRAFT_3618 0.32 +0.2 277,890 + EX28DRAFT_3618 0.32 -0.2 277,891 - EX28DRAFT_3618 0.32 +0.1 277,891 - EX28DRAFT_3618 0.32 +2.3 277,891 - EX28DRAFT_3618 0.32 +1.1 277,891 - EX28DRAFT_3618 0.32 +0.4 277,893 + EX28DRAFT_3618 0.32 +0.5 277,894 - EX28DRAFT_3618 0.32 -1.2 277,894 - EX28DRAFT_3618 0.32 -1.1 277,986 + EX28DRAFT_3618 0.44 -0.0
Or see this region's nucleotide sequence