Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3461 and EX28DRAFT_3462 are separated by 164 nucleotides EX28DRAFT_3462 and EX28DRAFT_3463 are separated by 46 nucleotides EX28DRAFT_3463 and EX28DRAFT_3464 are separated by 30 nucleotides
EX28DRAFT_3461: EX28DRAFT_3461 - ornithine carbamoyltransferase, at 134,882 to 135,886
_3461
EX28DRAFT_3462: EX28DRAFT_3462 - Uncharacterized protein conserved in bacteria, at 136,051 to 136,476
_3462
EX28DRAFT_3463: EX28DRAFT_3463 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 136,523 to 137,281
_3463
EX28DRAFT_3464: EX28DRAFT_3464 - hypothetical protein, at 137,312 to 138,034
_3464
Position (kb) 136
137 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 135.198 kb on + strand, within EX28DRAFT_3461 at 135.199 kb on - strand, within EX28DRAFT_3461 at 135.202 kb on + strand, within EX28DRAFT_3461 at 135.203 kb on - strand, within EX28DRAFT_3461 at 135.571 kb on - strand, within EX28DRAFT_3461 at 135.661 kb on - strand, within EX28DRAFT_3461 at 135.979 kb on + strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.101 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.145 kb on - strand, within EX28DRAFT_3462 at 136.333 kb on - strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.391 kb on - strand, within EX28DRAFT_3462 at 136.441 kb on + strand at 136.540 kb on - strand at 136.540 kb on - strand at 136.594 kb on + strand at 136.595 kb on - strand at 136.687 kb on + strand, within EX28DRAFT_3463 at 136.691 kb on + strand, within EX28DRAFT_3463 at 136.742 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.862 kb on - strand, within EX28DRAFT_3463 at 136.938 kb on - strand, within EX28DRAFT_3463 at 137.036 kb on + strand, within EX28DRAFT_3463 at 137.141 kb on + strand, within EX28DRAFT_3463 at 137.150 kb on + strand, within EX28DRAFT_3463 at 137.150 kb on + strand, within EX28DRAFT_3463 at 137.151 kb on - strand, within EX28DRAFT_3463 at 137.185 kb on + strand, within EX28DRAFT_3463 at 137.186 kb on - strand, within EX28DRAFT_3463 at 137.186 kb on - strand, within EX28DRAFT_3463 at 137.199 kb on + strand, within EX28DRAFT_3463 at 137.230 kb on + strand at 137.231 kb on - strand at 137.231 kb on - strand at 137.231 kb on - strand at 137.317 kb on + strand at 137.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 135,198 + EX28DRAFT_3461 0.31 -0.7 135,199 - EX28DRAFT_3461 0.32 -2.8 135,202 + EX28DRAFT_3461 0.32 -1.2 135,203 - EX28DRAFT_3461 0.32 -0.3 135,571 - EX28DRAFT_3461 0.69 -0.1 135,661 - EX28DRAFT_3461 0.78 -1.7 135,979 + +0.0 136,022 - +0.6 136,022 - -1.5 136,022 - +0.2 136,101 + EX28DRAFT_3462 0.12 -0.0 136,144 + EX28DRAFT_3462 0.22 -0.4 136,144 + EX28DRAFT_3462 0.22 +0.7 136,144 + EX28DRAFT_3462 0.22 +1.1 136,144 + EX28DRAFT_3462 0.22 -0.0 136,145 - EX28DRAFT_3462 0.22 +0.2 136,333 - EX28DRAFT_3462 0.66 +0.2 136,390 + EX28DRAFT_3462 0.80 +0.2 136,390 + EX28DRAFT_3462 0.80 +0.6 136,390 + EX28DRAFT_3462 0.80 +0.8 136,390 + EX28DRAFT_3462 0.80 +0.4 136,391 - EX28DRAFT_3462 0.80 +0.2 136,441 + +0.0 136,540 - -0.3 136,540 - +0.3 136,594 + -0.2 136,595 - -0.2 136,687 + EX28DRAFT_3463 0.22 +1.7 136,691 + EX28DRAFT_3463 0.22 +0.3 136,742 + EX28DRAFT_3463 0.29 +0.2 136,861 + EX28DRAFT_3463 0.45 -0.0 136,861 + EX28DRAFT_3463 0.45 +0.1 136,862 - EX28DRAFT_3463 0.45 +3.2 136,938 - EX28DRAFT_3463 0.55 -0.1 137,036 + EX28DRAFT_3463 0.68 +0.1 137,141 + EX28DRAFT_3463 0.81 -0.0 137,150 + EX28DRAFT_3463 0.83 -0.4 137,150 + EX28DRAFT_3463 0.83 +0.0 137,151 - EX28DRAFT_3463 0.83 -0.8 137,185 + EX28DRAFT_3463 0.87 -0.9 137,186 - EX28DRAFT_3463 0.87 +0.0 137,186 - EX28DRAFT_3463 0.87 -0.7 137,199 + EX28DRAFT_3463 0.89 +0.6 137,230 + +0.6 137,231 - +0.5 137,231 - -0.4 137,231 - -0.1 137,317 + -1.1 137,318 - -0.2
Or see this region's nucleotide sequence