Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3256 and EX28DRAFT_3257 are separated by 22 nucleotides EX28DRAFT_3257 and EX28DRAFT_3258 are separated by 82 nucleotides EX28DRAFT_3258 and EX28DRAFT_3259 are separated by 45 nucleotides EX28DRAFT_3259 and EX28DRAFT_3260 overlap by 4 nucleotides EX28DRAFT_3260 and EX28DRAFT_3261 are separated by 39 nucleotides
EX28DRAFT_3256: EX28DRAFT_3256 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 548,139 to 549,572
_3256
EX28DRAFT_3257: EX28DRAFT_3257 - ribosomal subunit interface protein, at 549,595 to 549,882
_3257
EX28DRAFT_3258: EX28DRAFT_3258 - PTS IIA-like nitrogen-regulatory protein PtsN, at 549,965 to 550,456
_3258
EX28DRAFT_3259: EX28DRAFT_3259 - Predicted P-loop-containing kinase, at 550,502 to 551,356
_3259
EX28DRAFT_3260: EX28DRAFT_3260 - Phosphotransferase System HPr (HPr) Family, at 551,353 to 551,625
_3260
EX28DRAFT_3261: EX28DRAFT_3261 - monofunctional biosynthetic peptidoglycan transglycosylase, at 551,665 to 552,390
_3261
Position (kb) 550
551
552 Strain fitness (log2 ratio) -1
0
1
2
3 at 549.595 kb on - strand at 549.595 kb on - strand at 549.682 kb on + strand, within EX28DRAFT_3257 at 549.682 kb on + strand, within EX28DRAFT_3257 at 549.706 kb on + strand, within EX28DRAFT_3257 at 549.706 kb on + strand, within EX28DRAFT_3257 at 549.706 kb on + strand, within EX28DRAFT_3257 at 549.706 kb on + strand, within EX28DRAFT_3257 at 549.706 kb on + strand, within EX28DRAFT_3257 at 549.707 kb on - strand, within EX28DRAFT_3257 at 549.707 kb on - strand, within EX28DRAFT_3257 at 549.708 kb on + strand, within EX28DRAFT_3257 at 549.708 kb on + strand, within EX28DRAFT_3257 at 549.709 kb on - strand, within EX28DRAFT_3257 at 549.738 kb on + strand, within EX28DRAFT_3257 at 549.738 kb on + strand, within EX28DRAFT_3257 at 549.805 kb on + strand, within EX28DRAFT_3257 at 549.805 kb on + strand, within EX28DRAFT_3257 at 549.805 kb on + strand, within EX28DRAFT_3257 at 549.806 kb on - strand, within EX28DRAFT_3257 at 549.806 kb on - strand, within EX28DRAFT_3257 at 549.858 kb on + strand at 549.880 kb on + strand at 549.880 kb on + strand at 549.881 kb on - strand at 549.947 kb on + strand at 550.413 kb on - strand at 550.420 kb on + strand at 550.676 kb on - strand, within EX28DRAFT_3259 at 551.155 kb on + strand, within EX28DRAFT_3259 at 551.215 kb on + strand, within EX28DRAFT_3259 at 551.216 kb on - strand, within EX28DRAFT_3259 at 551.633 kb on + strand at 551.666 kb on + strand at 551.667 kb on - strand at 551.667 kb on - strand at 551.670 kb on + strand at 551.671 kb on - strand at 551.671 kb on - strand at 551.671 kb on - strand at 551.671 kb on - strand at 551.676 kb on + strand at 551.676 kb on + strand at 551.677 kb on - strand at 551.677 kb on - strand at 551.677 kb on - strand at 551.677 kb on - strand at 551.677 kb on - strand at 551.680 kb on - strand at 551.725 kb on - strand at 551.751 kb on - strand, within EX28DRAFT_3261 at 551.751 kb on - strand, within EX28DRAFT_3261 at 551.852 kb on + strand, within EX28DRAFT_3261 at 551.881 kb on + strand, within EX28DRAFT_3261 at 551.912 kb on + strand, within EX28DRAFT_3261 at 552.001 kb on - strand, within EX28DRAFT_3261 at 552.215 kb on + strand, within EX28DRAFT_3261 at 552.295 kb on + strand, within EX28DRAFT_3261 at 552.296 kb on - strand, within EX28DRAFT_3261 at 552.335 kb on - strand at 552.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 549,595 - +0.9 549,595 - -0.2 549,682 + EX28DRAFT_3257 0.30 +0.2 549,682 + EX28DRAFT_3257 0.30 -0.1 549,706 + EX28DRAFT_3257 0.39 -0.8 549,706 + EX28DRAFT_3257 0.39 -1.5 549,706 + EX28DRAFT_3257 0.39 +1.9 549,706 + EX28DRAFT_3257 0.39 -0.1 549,706 + EX28DRAFT_3257 0.39 +0.8 549,707 - EX28DRAFT_3257 0.39 +0.8 549,707 - EX28DRAFT_3257 0.39 -0.4 549,708 + EX28DRAFT_3257 0.39 -0.5 549,708 + EX28DRAFT_3257 0.39 +0.6 549,709 - EX28DRAFT_3257 0.40 +0.5 549,738 + EX28DRAFT_3257 0.50 -1.5 549,738 + EX28DRAFT_3257 0.50 +0.3 549,805 + EX28DRAFT_3257 0.73 +0.4 549,805 + EX28DRAFT_3257 0.73 -0.3 549,805 + EX28DRAFT_3257 0.73 +0.6 549,806 - EX28DRAFT_3257 0.73 +0.8 549,806 - EX28DRAFT_3257 0.73 +0.7 549,858 + -0.1 549,880 + +3.6 549,880 + -0.3 549,881 - +1.1 549,947 + -0.9 550,413 - +2.0 550,420 + -1.0 550,676 - EX28DRAFT_3259 0.20 -0.8 551,155 + EX28DRAFT_3259 0.76 -0.8 551,215 + EX28DRAFT_3259 0.83 -1.0 551,216 - EX28DRAFT_3259 0.84 -1.6 551,633 + -0.0 551,666 + -1.4 551,667 - -0.3 551,667 - -0.7 551,670 + +0.8 551,671 - -0.2 551,671 - +0.3 551,671 - +0.5 551,671 - +0.6 551,676 + -0.7 551,676 + +0.2 551,677 - -0.0 551,677 - +0.4 551,677 - +0.1 551,677 - -0.4 551,677 - +0.6 551,680 - -0.0 551,725 - +0.8 551,751 - EX28DRAFT_3261 0.12 +0.2 551,751 - EX28DRAFT_3261 0.12 +0.3 551,852 + EX28DRAFT_3261 0.26 +0.8 551,881 + EX28DRAFT_3261 0.30 +0.3 551,912 + EX28DRAFT_3261 0.34 +0.5 552,001 - EX28DRAFT_3261 0.46 +0.5 552,215 + EX28DRAFT_3261 0.76 -0.8 552,295 + EX28DRAFT_3261 0.87 -0.2 552,296 - EX28DRAFT_3261 0.87 -1.0 552,335 - +0.9 552,335 - +0.3
Or see this region's nucleotide sequence