Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2757 and EX28DRAFT_2758 are separated by 160 nucleotides EX28DRAFT_2758 and EX28DRAFT_2759 are separated by 243 nucleotides EX28DRAFT_2759 and EX28DRAFT_2760 overlap by 4 nucleotides EX28DRAFT_2760 and EX28DRAFT_2761 overlap by 28 nucleotides
EX28DRAFT_2757: EX28DRAFT_2757 - Protein of unknown function (DUF2002), at 65,088 to 65,432
_2757
EX28DRAFT_2758: EX28DRAFT_2758 - Uncharacterized conserved protein, at 65,593 to 65,919
_2758
EX28DRAFT_2759: EX28DRAFT_2759 - ribonucleoside-diphosphate reductase class Ib glutaredoxin subunit (EC 1.17.4.1), at 66,163 to 66,402
_2759
EX28DRAFT_2760: EX28DRAFT_2760 - ribonucleoside-diphosphate reductase 2, operon protein nrdI, at 66,399 to 66,809
_2760
EX28DRAFT_2761: EX28DRAFT_2761 - ribonucleoside-diphosphate reductase, alpha subunit, at 66,782 to 68,926
_2761
Position (kb) 66
67 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 65.428 kb on + strand at 65.428 kb on + strand at 65.428 kb on + strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.433 kb on - strand at 65.469 kb on + strand at 65.469 kb on + strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.474 kb on + strand at 65.485 kb on - strand at 65.485 kb on - strand at 65.501 kb on + strand at 65.541 kb on + strand at 65.541 kb on + strand at 65.545 kb on - strand at 65.620 kb on - strand at 65.853 kb on - strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.918 kb on - strand at 66.033 kb on - strand at 66.078 kb on - strand at 66.078 kb on - strand at 66.104 kb on - strand at 66.104 kb on - strand at 66.399 kb on - strand at 66.414 kb on + strand at 66.414 kb on + strand at 66.512 kb on - strand, within EX28DRAFT_2760 at 66.522 kb on + strand, within EX28DRAFT_2760 at 66.543 kb on + strand, within EX28DRAFT_2760 at 66.764 kb on + strand, within EX28DRAFT_2760 at 66.858 kb on - strand at 66.992 kb on + strand at 66.993 kb on - strand at 67.106 kb on + strand, within EX28DRAFT_2761 at 67.107 kb on - strand, within EX28DRAFT_2761 at 67.115 kb on + strand, within EX28DRAFT_2761 at 67.116 kb on - strand, within EX28DRAFT_2761 at 67.118 kb on - strand, within EX28DRAFT_2761 at 67.146 kb on - strand, within EX28DRAFT_2761 at 67.150 kb on - strand, within EX28DRAFT_2761 at 67.327 kb on + strand, within EX28DRAFT_2761 at 67.339 kb on + strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.512 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.688 kb on - strand, within EX28DRAFT_2761 at 67.729 kb on + strand, within EX28DRAFT_2761
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 65,428 + -0.4 65,428 + -1.0 65,428 + -0.2 65,429 - -1.3 65,429 - +0.5 65,429 - +0.2 65,433 - +0.3 65,469 + -0.1 65,469 + -0.7 65,470 - -0.1 65,470 - -0.3 65,470 - -1.4 65,474 + +0.1 65,485 - -0.2 65,485 - -0.9 65,501 + -0.2 65,541 + +0.3 65,541 + -0.1 65,545 - -0.0 65,620 - -0.1 65,853 - EX28DRAFT_2758 0.80 -0.2 65,856 + EX28DRAFT_2758 0.80 -3.9 65,856 + EX28DRAFT_2758 0.80 +0.2 65,856 + EX28DRAFT_2758 0.80 +0.5 65,857 - EX28DRAFT_2758 0.81 -0.2 65,857 - EX28DRAFT_2758 0.81 -0.3 65,918 - +0.1 66,033 - -0.5 66,078 - -0.4 66,078 - +0.2 66,104 - +0.7 66,104 - -0.2 66,399 - +0.5 66,414 + -1.3 66,414 + +0.4 66,512 - EX28DRAFT_2760 0.27 +1.0 66,522 + EX28DRAFT_2760 0.30 +0.8 66,543 + EX28DRAFT_2760 0.35 +0.1 66,764 + EX28DRAFT_2760 0.89 +0.1 66,858 - +1.3 66,992 + -1.2 66,993 - +0.0 67,106 + EX28DRAFT_2761 0.15 -0.8 67,107 - EX28DRAFT_2761 0.15 +0.0 67,115 + EX28DRAFT_2761 0.16 -0.1 67,116 - EX28DRAFT_2761 0.16 +0.4 67,118 - EX28DRAFT_2761 0.16 +0.2 67,146 - EX28DRAFT_2761 0.17 +0.1 67,150 - EX28DRAFT_2761 0.17 +0.5 67,327 + EX28DRAFT_2761 0.25 +0.4 67,339 + EX28DRAFT_2761 0.26 +0.1 67,340 - EX28DRAFT_2761 0.26 -0.0 67,340 - EX28DRAFT_2761 0.26 +0.5 67,512 - EX28DRAFT_2761 0.34 +0.0 67,673 - EX28DRAFT_2761 0.42 +0.1 67,673 - EX28DRAFT_2761 0.42 +0.3 67,688 - EX28DRAFT_2761 0.42 +0.1 67,729 + EX28DRAFT_2761 0.44 -0.9
Or see this region's nucleotide sequence