Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2701 and EX28DRAFT_2702 are separated by 10 nucleotides EX28DRAFT_2702 and EX28DRAFT_2703 are separated by 214 nucleotides EX28DRAFT_2703 and EX28DRAFT_2704 are separated by 92 nucleotides
EX28DRAFT_2701: EX28DRAFT_2701 - chorismate mutase (EC 5.4.99.5), at 8,751 to 9,872
_2701
EX28DRAFT_2702: EX28DRAFT_2702 - 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (EC 2.5.1.54), at 9,883 to 10,953
_2702
EX28DRAFT_2703: EX28DRAFT_2703 - Protein of unknown function (DUF2799), at 11,168 to 11,542
_2703
EX28DRAFT_2704: EX28DRAFT_2704 - Domain of unknown function (DUF4154), at 11,635 to 12,231
_2704
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 8.911 kb on + strand, within EX28DRAFT_2701 at 8.912 kb on - strand, within EX28DRAFT_2701 at 8.951 kb on + strand, within EX28DRAFT_2701 at 8.952 kb on - strand, within EX28DRAFT_2701 at 8.952 kb on - strand, within EX28DRAFT_2701 at 8.952 kb on - strand, within EX28DRAFT_2701 at 8.955 kb on - strand, within EX28DRAFT_2701 at 8.966 kb on - strand, within EX28DRAFT_2701 at 9.020 kb on - strand, within EX28DRAFT_2701 at 9.020 kb on - strand, within EX28DRAFT_2701 at 9.020 kb on - strand, within EX28DRAFT_2701 at 9.023 kb on + strand, within EX28DRAFT_2701 at 9.625 kb on + strand, within EX28DRAFT_2701 at 9.872 kb on + strand at 9.873 kb on - strand at 9.877 kb on - strand at 9.884 kb on + strand at 9.885 kb on - strand at 10.031 kb on - strand, within EX28DRAFT_2702 at 10.134 kb on + strand, within EX28DRAFT_2702 at 10.159 kb on + strand, within EX28DRAFT_2702 at 10.160 kb on - strand, within EX28DRAFT_2702 at 10.233 kb on + strand, within EX28DRAFT_2702 at 10.233 kb on + strand, within EX28DRAFT_2702 at 10.297 kb on + strand, within EX28DRAFT_2702 at 10.297 kb on + strand, within EX28DRAFT_2702 at 10.298 kb on - strand, within EX28DRAFT_2702 at 10.689 kb on - strand, within EX28DRAFT_2702 at 10.737 kb on - strand, within EX28DRAFT_2702 at 10.780 kb on + strand, within EX28DRAFT_2702 at 10.924 kb on - strand at 10.924 kb on - strand at 10.924 kb on - strand at 10.924 kb on - strand at 10.927 kb on + strand at 10.927 kb on + strand at 10.928 kb on - strand at 10.928 kb on - strand at 10.928 kb on - strand at 10.928 kb on - strand at 10.928 kb on - strand at 10.928 kb on - strand at 11.076 kb on + strand at 11.311 kb on + strand, within EX28DRAFT_2703 at 11.364 kb on - strand, within EX28DRAFT_2703 at 11.413 kb on + strand, within EX28DRAFT_2703 at 11.518 kb on + strand at 11.518 kb on + strand at 11.540 kb on + strand at 11.545 kb on + strand at 11.576 kb on - strand at 11.588 kb on - strand at 11.604 kb on - strand at 11.614 kb on + strand at 11.615 kb on - strand at 11.657 kb on + strand at 11.657 kb on + strand at 11.667 kb on + strand at 11.668 kb on - strand at 11.668 kb on - strand at 11.668 kb on - strand at 11.668 kb on - strand at 11.754 kb on + strand, within EX28DRAFT_2704 at 11.754 kb on + strand, within EX28DRAFT_2704 at 11.755 kb on - strand, within EX28DRAFT_2704 at 11.755 kb on - strand, within EX28DRAFT_2704 at 11.784 kb on + strand, within EX28DRAFT_2704 at 11.866 kb on - strand, within EX28DRAFT_2704 at 11.896 kb on - strand, within EX28DRAFT_2704 at 11.934 kb on - strand, within EX28DRAFT_2704 at 11.939 kb on - strand, within EX28DRAFT_2704 at 11.939 kb on - strand, within EX28DRAFT_2704 at 11.941 kb on - strand, within EX28DRAFT_2704
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 8,911 + EX28DRAFT_2701 0.14 -2.0 8,912 - EX28DRAFT_2701 0.14 -1.7 8,951 + EX28DRAFT_2701 0.18 -3.0 8,952 - EX28DRAFT_2701 0.18 -1.9 8,952 - EX28DRAFT_2701 0.18 -0.9 8,952 - EX28DRAFT_2701 0.18 -2.3 8,955 - EX28DRAFT_2701 0.18 -1.0 8,966 - EX28DRAFT_2701 0.19 -3.4 9,020 - EX28DRAFT_2701 0.24 -3.2 9,020 - EX28DRAFT_2701 0.24 -1.6 9,020 - EX28DRAFT_2701 0.24 -1.9 9,023 + EX28DRAFT_2701 0.24 -2.5 9,625 + EX28DRAFT_2701 0.78 -1.7 9,872 + -3.1 9,873 - -3.3 9,877 - -3.9 9,884 + +0.4 9,885 - +0.6 10,031 - EX28DRAFT_2702 0.14 -2.3 10,134 + EX28DRAFT_2702 0.23 -2.9 10,159 + EX28DRAFT_2702 0.26 -2.4 10,160 - EX28DRAFT_2702 0.26 -1.6 10,233 + EX28DRAFT_2702 0.33 -0.6 10,233 + EX28DRAFT_2702 0.33 -1.2 10,297 + EX28DRAFT_2702 0.39 -2.3 10,297 + EX28DRAFT_2702 0.39 -3.4 10,298 - EX28DRAFT_2702 0.39 -2.6 10,689 - EX28DRAFT_2702 0.75 -1.9 10,737 - EX28DRAFT_2702 0.80 -1.9 10,780 + EX28DRAFT_2702 0.84 -2.8 10,924 - -0.2 10,924 - +0.0 10,924 - -1.0 10,924 - +0.7 10,927 + -2.0 10,927 + -1.1 10,928 - -3.0 10,928 - +0.2 10,928 - -2.2 10,928 - -0.6 10,928 - -1.6 10,928 - -1.9 11,076 + +0.3 11,311 + EX28DRAFT_2703 0.38 -1.6 11,364 - EX28DRAFT_2703 0.52 +0.7 11,413 + EX28DRAFT_2703 0.65 -0.1 11,518 + -0.5 11,518 + -0.8 11,540 + -1.1 11,545 + -0.1 11,576 - -0.1 11,588 - +0.1 11,604 - -0.5 11,614 + +0.0 11,615 - -0.9 11,657 + +0.5 11,657 + +0.3 11,667 + -0.6 11,668 - -0.1 11,668 - -1.7 11,668 - -0.9 11,668 - +0.6 11,754 + EX28DRAFT_2704 0.20 -0.3 11,754 + EX28DRAFT_2704 0.20 +1.2 11,755 - EX28DRAFT_2704 0.20 -0.4 11,755 - EX28DRAFT_2704 0.20 +2.2 11,784 + EX28DRAFT_2704 0.25 -0.6 11,866 - EX28DRAFT_2704 0.39 -0.1 11,896 - EX28DRAFT_2704 0.44 +1.5 11,934 - EX28DRAFT_2704 0.50 -0.8 11,939 - EX28DRAFT_2704 0.51 -0.1 11,939 - EX28DRAFT_2704 0.51 +0.7 11,941 - EX28DRAFT_2704 0.51 -0.7
Or see this region's nucleotide sequence