Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2324 and EX28DRAFT_2325 are separated by 1 nucleotides EX28DRAFT_2325 and EX28DRAFT_2326 are separated by 149 nucleotides EX28DRAFT_2326 and EX28DRAFT_2327 are separated by 3 nucleotides EX28DRAFT_2327 and EX28DRAFT_2328 are separated by 28 nucleotides
EX28DRAFT_2324: EX28DRAFT_2324 - DNA polymerase III, delta subunit (EC 2.7.7.7), at 2,349,246 to 2,350,280
_2324
EX28DRAFT_2325: EX28DRAFT_2325 - nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 2,350,282 to 2,350,938
_2325
EX28DRAFT_2326: EX28DRAFT_2326 - ribosome silencing factor RsfS/YbeB/iojap, at 2,351,088 to 2,351,525
_2326
EX28DRAFT_2327: EX28DRAFT_2327 - rRNA large subunit m3Psi methyltransferase RlmH, at 2,351,529 to 2,351,996
_2327
EX28DRAFT_2328: EX28DRAFT_2328 - penicillin-binding protein 2, at 2,352,025 to 2,353,926
_2328
Position (kb)
2351
2352 Strain fitness (log2 ratio)
-1
0
1 at 2350.949 kb on + strand at 2350.993 kb on + strand at 2350.994 kb on - strand at 2351.150 kb on + strand, within EX28DRAFT_2326 at 2351.150 kb on + strand, within EX28DRAFT_2326 at 2351.152 kb on + strand, within EX28DRAFT_2326 at 2351.179 kb on + strand, within EX28DRAFT_2326 at 2351.303 kb on + strand, within EX28DRAFT_2326 at 2351.333 kb on + strand, within EX28DRAFT_2326
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,350,949 + -0.7 2,350,993 + -0.3 2,350,994 - -0.4 2,351,150 + EX28DRAFT_2326 0.14 +0.9 2,351,150 + EX28DRAFT_2326 0.14 +0.2 2,351,152 + EX28DRAFT_2326 0.15 -0.1 2,351,179 + EX28DRAFT_2326 0.21 +1.0 2,351,303 + EX28DRAFT_2326 0.49 -1.6 2,351,333 + EX28DRAFT_2326 0.56 +1.1
Or see this region's nucleotide sequence