Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2092 and EX28DRAFT_2093 overlap by 17 nucleotides EX28DRAFT_2093 and EX28DRAFT_2094 are separated by 116 nucleotides EX28DRAFT_2094 and EX28DRAFT_2095 are separated by 40 nucleotides EX28DRAFT_2095 and EX28DRAFT_2096 are separated by 65 nucleotides
EX28DRAFT_2092: EX28DRAFT_2092 - His Kinase A (phospho-acceptor) domain/Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase, at 2,115,872 to 2,117,341
_2092
EX28DRAFT_2093: EX28DRAFT_2093 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,117,325 to 2,118,032
_2093
EX28DRAFT_2094: EX28DRAFT_2094 - 23S rRNA (uracil-5-)-methyltransferase RumB, at 2,118,149 to 2,119,279
_2094
EX28DRAFT_2095: EX28DRAFT_2095 - Protein of unknown function (DUF2593), at 2,119,320 to 2,119,793
_2095
EX28DRAFT_2096: EX28DRAFT_2096 - ABC-type spermidine/putrescine transport system, permease component II, at 2,119,859 to 2,120,704
_2096
Position (kb)
2118
2119
2120 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2117.183 kb on + strand, within EX28DRAFT_2092 at 2117.184 kb on - strand, within EX28DRAFT_2092 at 2117.362 kb on + strand at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.439 kb on - strand, within EX28DRAFT_2093 at 2117.447 kb on + strand, within EX28DRAFT_2093 at 2117.737 kb on + strand, within EX28DRAFT_2093 at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.068 kb on + strand at 2118.193 kb on + strand at 2118.205 kb on + strand at 2118.288 kb on + strand, within EX28DRAFT_2094 at 2118.297 kb on + strand, within EX28DRAFT_2094 at 2118.321 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.572 kb on - strand, within EX28DRAFT_2094 at 2119.108 kb on + strand, within EX28DRAFT_2094 at 2119.229 kb on + strand at 2119.263 kb on + strand at 2119.280 kb on - strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.315 kb on - strand at 2119.321 kb on + strand at 2119.489 kb on + strand, within EX28DRAFT_2095 at 2119.709 kb on + strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.712 kb on + strand, within EX28DRAFT_2095 at 2119.746 kb on + strand at 2119.746 kb on + strand at 2119.747 kb on - strand at 2119.747 kb on - strand at 2119.752 kb on - strand at 2119.766 kb on + strand at 2119.766 kb on + strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.770 kb on - strand at 2119.799 kb on + strand at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.964 kb on - strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.245 kb on - strand, within EX28DRAFT_2096 at 2120.252 kb on - strand, within EX28DRAFT_2096
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,117,183 + EX28DRAFT_2092 0.89 +0.4 2,117,184 - EX28DRAFT_2092 0.89 +0.6 2,117,362 + -0.4 2,117,438 + EX28DRAFT_2093 0.16 +0.6 2,117,438 + EX28DRAFT_2093 0.16 -0.1 2,117,439 - EX28DRAFT_2093 0.16 -1.1 2,117,447 + EX28DRAFT_2093 0.17 +0.2 2,117,737 + EX28DRAFT_2093 0.58 +0.1 2,118,056 - +0.8 2,118,056 - +0.2 2,118,056 - +0.0 2,118,063 - +0.4 2,118,063 - +0.9 2,118,063 - +2.7 2,118,063 - -0.2 2,118,068 + +0.2 2,118,193 + -0.4 2,118,205 + +0.4 2,118,288 + EX28DRAFT_2094 0.12 -0.6 2,118,297 + EX28DRAFT_2094 0.13 -1.1 2,118,321 + EX28DRAFT_2094 0.15 -0.3 2,118,571 + EX28DRAFT_2094 0.37 -0.4 2,118,571 + EX28DRAFT_2094 0.37 -0.2 2,118,571 + EX28DRAFT_2094 0.37 +0.1 2,118,572 - EX28DRAFT_2094 0.37 +0.4 2,119,108 + EX28DRAFT_2094 0.85 -0.1 2,119,229 + +0.8 2,119,263 + +0.0 2,119,280 - -0.6 2,119,314 + +0.4 2,119,314 + -1.1 2,119,314 + +0.6 2,119,315 - +1.1 2,119,321 + -1.1 2,119,489 + EX28DRAFT_2095 0.36 -0.0 2,119,709 + EX28DRAFT_2095 0.82 -0.2 2,119,710 - EX28DRAFT_2095 0.82 -0.3 2,119,710 - EX28DRAFT_2095 0.82 +0.6 2,119,710 - EX28DRAFT_2095 0.82 +0.0 2,119,712 + EX28DRAFT_2095 0.83 +0.6 2,119,746 + +0.1 2,119,746 + +1.0 2,119,747 - +0.3 2,119,747 - +0.6 2,119,752 - +0.3 2,119,766 + -0.1 2,119,766 + -0.0 2,119,767 - -1.4 2,119,767 - -0.2 2,119,767 - +0.7 2,119,770 - +0.7 2,119,799 + +0.3 2,119,963 + EX28DRAFT_2096 0.12 -0.3 2,119,963 + EX28DRAFT_2096 0.12 -0.5 2,119,964 - EX28DRAFT_2096 0.12 -1.7 2,120,244 + EX28DRAFT_2096 0.46 +0.4 2,120,244 + EX28DRAFT_2096 0.46 +3.2 2,120,245 - EX28DRAFT_2096 0.46 +0.3 2,120,252 - EX28DRAFT_2096 0.46 +0.1
Or see this region's nucleotide sequence