Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1648 and EX28DRAFT_1649 are separated by 100 nucleotides EX28DRAFT_1649 and EX28DRAFT_1650 are separated by 95 nucleotides EX28DRAFT_1650 and EX28DRAFT_1651 are separated by 44 nucleotides EX28DRAFT_1651 and EX28DRAFT_1652 are separated by 338 nucleotides
EX28DRAFT_1648: EX28DRAFT_1648 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,664,809 to 1,665,906
_1648
EX28DRAFT_1649: EX28DRAFT_1649 - lactoylglutathione lyase, at 1,666,007 to 1,666,414
_1649
EX28DRAFT_1650: EX28DRAFT_1650 - ribonuclease T, at 1,666,510 to 1,667,160
_1650
EX28DRAFT_1651: EX28DRAFT_1651 - monothiol glutaredoxin, Grx4 family, at 1,667,205 to 1,667,552
_1651
EX28DRAFT_1652: EX28DRAFT_1652 - Cell wall-associated hydrolases (invasion-associated proteins), at 1,667,891 to 1,668,664
_1652
Position (kb)
1666
1667
1668 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1665.574 kb on + strand, within EX28DRAFT_1648 at 1665.718 kb on + strand, within EX28DRAFT_1648 at 1665.719 kb on - strand, within EX28DRAFT_1648 at 1665.721 kb on - strand, within EX28DRAFT_1648 at 1665.759 kb on - strand, within EX28DRAFT_1648 at 1665.883 kb on + strand at 1665.893 kb on - strand at 1665.982 kb on + strand at 1665.983 kb on - strand at 1665.983 kb on - strand at 1666.006 kb on + strand at 1666.007 kb on - strand at 1666.069 kb on + strand, within EX28DRAFT_1649 at 1666.121 kb on + strand, within EX28DRAFT_1649 at 1666.122 kb on - strand, within EX28DRAFT_1649 at 1666.122 kb on - strand, within EX28DRAFT_1649 at 1666.181 kb on + strand, within EX28DRAFT_1649 at 1666.182 kb on - strand, within EX28DRAFT_1649 at 1666.182 kb on - strand, within EX28DRAFT_1649 at 1666.183 kb on + strand, within EX28DRAFT_1649 at 1666.184 kb on - strand, within EX28DRAFT_1649 at 1666.202 kb on + strand, within EX28DRAFT_1649 at 1666.202 kb on + strand, within EX28DRAFT_1649 at 1666.203 kb on - strand, within EX28DRAFT_1649 at 1666.315 kb on - strand, within EX28DRAFT_1649 at 1666.440 kb on + strand at 1666.498 kb on + strand at 1666.786 kb on + strand, within EX28DRAFT_1650 at 1667.004 kb on + strand, within EX28DRAFT_1650 at 1667.191 kb on - strand at 1667.206 kb on + strand at 1667.282 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.360 kb on - strand, within EX28DRAFT_1651 at 1667.400 kb on - strand, within EX28DRAFT_1651 at 1667.492 kb on + strand, within EX28DRAFT_1651 at 1667.493 kb on - strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.494 kb on + strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.495 kb on - strand, within EX28DRAFT_1651 at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.567 kb on - strand at 1667.641 kb on + strand at 1667.641 kb on + strand at 1667.643 kb on + strand at 1667.644 kb on - strand at 1667.650 kb on + strand at 1667.688 kb on + strand at 1667.780 kb on + strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.781 kb on - strand at 1667.796 kb on - strand at 1667.798 kb on + strand at 1667.814 kb on + strand at 1667.821 kb on + strand at 1667.822 kb on - strand at 1667.822 kb on - strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.839 kb on + strand at 1667.931 kb on + strand at 1667.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,665,574 + EX28DRAFT_1648 0.70 -0.4 1,665,718 + EX28DRAFT_1648 0.83 -0.6 1,665,719 - EX28DRAFT_1648 0.83 +0.3 1,665,721 - EX28DRAFT_1648 0.83 -0.1 1,665,759 - EX28DRAFT_1648 0.87 -0.1 1,665,883 + -0.3 1,665,893 - -0.3 1,665,982 + +0.1 1,665,983 - +0.2 1,665,983 - -0.6 1,666,006 + +0.3 1,666,007 - -0.3 1,666,069 + EX28DRAFT_1649 0.15 +1.0 1,666,121 + EX28DRAFT_1649 0.28 -1.5 1,666,122 - EX28DRAFT_1649 0.28 +0.1 1,666,122 - EX28DRAFT_1649 0.28 -0.6 1,666,181 + EX28DRAFT_1649 0.43 -0.5 1,666,182 - EX28DRAFT_1649 0.43 -0.0 1,666,182 - EX28DRAFT_1649 0.43 +0.3 1,666,183 + EX28DRAFT_1649 0.43 +0.1 1,666,184 - EX28DRAFT_1649 0.43 -0.3 1,666,202 + EX28DRAFT_1649 0.48 -0.1 1,666,202 + EX28DRAFT_1649 0.48 -0.2 1,666,203 - EX28DRAFT_1649 0.48 -0.6 1,666,315 - EX28DRAFT_1649 0.75 -0.3 1,666,440 + +0.6 1,666,498 + +0.4 1,666,786 + EX28DRAFT_1650 0.42 -2.2 1,667,004 + EX28DRAFT_1650 0.76 -1.2 1,667,191 - +0.3 1,667,206 + +0.2 1,667,282 - EX28DRAFT_1651 0.22 +0.1 1,667,360 - EX28DRAFT_1651 0.45 +0.4 1,667,360 - EX28DRAFT_1651 0.45 -0.2 1,667,400 - EX28DRAFT_1651 0.56 -0.7 1,667,492 + EX28DRAFT_1651 0.82 -2.1 1,667,493 - EX28DRAFT_1651 0.83 -0.1 1,667,494 + EX28DRAFT_1651 0.83 -0.7 1,667,494 + EX28DRAFT_1651 0.83 -0.7 1,667,495 - EX28DRAFT_1651 0.83 -2.9 1,667,495 - EX28DRAFT_1651 0.83 -1.0 1,667,567 - +0.3 1,667,567 - -0.3 1,667,567 - +1.1 1,667,567 - -0.7 1,667,641 + +0.1 1,667,641 + -0.1 1,667,643 + -0.6 1,667,644 - -0.3 1,667,650 + +1.0 1,667,688 + +0.3 1,667,780 + -0.2 1,667,781 - +1.2 1,667,781 - +0.6 1,667,781 - +1.2 1,667,781 - -2.0 1,667,796 - -0.2 1,667,798 + -1.7 1,667,814 + -0.2 1,667,821 + +0.1 1,667,822 - +0.4 1,667,822 - -0.4 1,667,839 + -1.3 1,667,839 + +0.2 1,667,839 + +0.2 1,667,931 + -0.5 1,667,938 + -0.5
Or see this region's nucleotide sequence