Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1644 and EX28DRAFT_1645 are separated by 48 nucleotides EX28DRAFT_1645 and EX28DRAFT_1646 are separated by 149 nucleotides EX28DRAFT_1646 and EX28DRAFT_1647 are separated by 41 nucleotides EX28DRAFT_1647 and EX28DRAFT_1648 are separated by 53 nucleotides
EX28DRAFT_1644: EX28DRAFT_1644 - Predicted oxidoreductase, at 1,661,155 to 1,662,051
_1644
EX28DRAFT_1645: EX28DRAFT_1645 - Predicted Fe-S protein, at 1,662,100 to 1,662,339
_1645
EX28DRAFT_1646: EX28DRAFT_1646 - Predicted membrane-associated, metal-dependent hydrolase, at 1,662,489 to 1,664,114
_1646
EX28DRAFT_1647: EX28DRAFT_1647 - transcriptional regulator, TetR family, at 1,664,156 to 1,664,755
_1647
EX28DRAFT_1648: EX28DRAFT_1648 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,664,809 to 1,665,906
_1648
Position (kb)
1662
1663
1664
1665 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1661.756 kb on + strand, within EX28DRAFT_1644 at 1661.889 kb on - strand, within EX28DRAFT_1644 at 1661.893 kb on - strand, within EX28DRAFT_1644 at 1661.967 kb on + strand at 1661.967 kb on + strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.968 kb on - strand at 1661.990 kb on + strand at 1661.991 kb on - strand at 1661.999 kb on - strand at 1662.036 kb on + strand at 1662.071 kb on + strand at 1662.071 kb on + strand at 1662.093 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.403 kb on + strand at 1662.404 kb on - strand at 1662.404 kb on - strand at 1662.449 kb on + strand at 1662.652 kb on + strand, within EX28DRAFT_1646 at 1662.653 kb on - strand, within EX28DRAFT_1646 at 1662.686 kb on + strand, within EX28DRAFT_1646 at 1662.697 kb on - strand, within EX28DRAFT_1646 at 1662.722 kb on - strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.750 kb on + strand, within EX28DRAFT_1646 at 1662.751 kb on - strand, within EX28DRAFT_1646 at 1662.755 kb on + strand, within EX28DRAFT_1646 at 1662.755 kb on + strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.756 kb on - strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.759 kb on + strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.760 kb on - strand, within EX28DRAFT_1646 at 1662.766 kb on - strand, within EX28DRAFT_1646 at 1662.766 kb on - strand, within EX28DRAFT_1646 at 1663.005 kb on - strand, within EX28DRAFT_1646 at 1663.012 kb on - strand, within EX28DRAFT_1646 at 1663.068 kb on + strand, within EX28DRAFT_1646 at 1663.069 kb on - strand, within EX28DRAFT_1646 at 1663.069 kb on - strand, within EX28DRAFT_1646 at 1663.089 kb on + strand, within EX28DRAFT_1646 at 1663.089 kb on + strand, within EX28DRAFT_1646 at 1663.089 kb on + strand, within EX28DRAFT_1646 at 1663.231 kb on - strand, within EX28DRAFT_1646 at 1663.290 kb on - strand, within EX28DRAFT_1646 at 1663.372 kb on - strand, within EX28DRAFT_1646 at 1663.406 kb on - strand, within EX28DRAFT_1646 at 1663.467 kb on - strand, within EX28DRAFT_1646 at 1663.563 kb on + strand, within EX28DRAFT_1646 at 1663.601 kb on + strand, within EX28DRAFT_1646 at 1663.644 kb on + strand, within EX28DRAFT_1646 at 1663.645 kb on - strand, within EX28DRAFT_1646 at 1663.673 kb on + strand, within EX28DRAFT_1646 at 1663.736 kb on + strand, within EX28DRAFT_1646 at 1663.736 kb on + strand, within EX28DRAFT_1646 at 1663.737 kb on - strand, within EX28DRAFT_1646 at 1663.737 kb on - strand, within EX28DRAFT_1646 at 1663.740 kb on + strand, within EX28DRAFT_1646 at 1663.741 kb on - strand, within EX28DRAFT_1646 at 1663.741 kb on - strand, within EX28DRAFT_1646 at 1663.759 kb on - strand, within EX28DRAFT_1646 at 1663.759 kb on - strand, within EX28DRAFT_1646 at 1663.871 kb on + strand, within EX28DRAFT_1646 at 1663.875 kb on + strand, within EX28DRAFT_1646 at 1663.923 kb on - strand, within EX28DRAFT_1646 at 1663.932 kb on - strand, within EX28DRAFT_1646 at 1664.030 kb on - strand at 1664.091 kb on - strand at 1664.092 kb on + strand at 1664.092 kb on + strand at 1664.093 kb on - strand at 1664.093 kb on - strand at 1664.093 kb on - strand at 1664.094 kb on - strand at 1664.167 kb on + strand at 1664.297 kb on + strand, within EX28DRAFT_1647 at 1664.395 kb on + strand, within EX28DRAFT_1647 at 1664.395 kb on + strand, within EX28DRAFT_1647 at 1664.420 kb on + strand, within EX28DRAFT_1647 at 1664.519 kb on - strand, within EX28DRAFT_1647 at 1664.536 kb on + strand, within EX28DRAFT_1647 at 1664.643 kb on + strand, within EX28DRAFT_1647 at 1664.644 kb on - strand, within EX28DRAFT_1647 at 1664.645 kb on + strand, within EX28DRAFT_1647 at 1664.646 kb on - strand, within EX28DRAFT_1647 at 1664.665 kb on + strand, within EX28DRAFT_1647 at 1664.738 kb on - strand at 1664.738 kb on - strand at 1664.754 kb on - strand at 1664.754 kb on - strand at 1664.766 kb on + strand at 1664.770 kb on + strand at 1664.808 kb on + strand at 1664.809 kb on - strand at 1664.830 kb on - strand at 1664.871 kb on - strand at 1664.871 kb on - strand at 1664.874 kb on + strand at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.941 kb on + strand, within EX28DRAFT_1648 at 1664.942 kb on - strand, within EX28DRAFT_1648
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,661,756 + EX28DRAFT_1644 0.67 +0.1 1,661,889 - EX28DRAFT_1644 0.82 +0.4 1,661,893 - EX28DRAFT_1644 0.82 -2.3 1,661,967 + -0.7 1,661,967 + -0.2 1,661,968 - -3.4 1,661,968 - +0.4 1,661,968 - -0.1 1,661,990 + +0.2 1,661,991 - +0.1 1,661,999 - +1.0 1,662,036 + -0.6 1,662,071 + +1.5 1,662,071 + +0.3 1,662,093 + -0.2 1,662,403 + -1.3 1,662,403 + -0.6 1,662,403 + -0.1 1,662,403 + -1.4 1,662,403 + -0.7 1,662,404 - +0.8 1,662,404 - -0.0 1,662,449 + -0.0 1,662,652 + EX28DRAFT_1646 0.10 -0.9 1,662,653 - EX28DRAFT_1646 0.10 -1.0 1,662,686 + EX28DRAFT_1646 0.12 +0.5 1,662,697 - EX28DRAFT_1646 0.13 -0.2 1,662,722 - EX28DRAFT_1646 0.14 -1.2 1,662,750 + EX28DRAFT_1646 0.16 -0.3 1,662,750 + EX28DRAFT_1646 0.16 +0.1 1,662,750 + EX28DRAFT_1646 0.16 -0.0 1,662,751 - EX28DRAFT_1646 0.16 -0.3 1,662,755 + EX28DRAFT_1646 0.16 +0.2 1,662,755 + EX28DRAFT_1646 0.16 +0.2 1,662,756 - EX28DRAFT_1646 0.16 -0.5 1,662,756 - EX28DRAFT_1646 0.16 +0.3 1,662,756 - EX28DRAFT_1646 0.16 -1.1 1,662,756 - EX28DRAFT_1646 0.16 +0.0 1,662,759 + EX28DRAFT_1646 0.17 +0.0 1,662,759 + EX28DRAFT_1646 0.17 -0.0 1,662,759 + EX28DRAFT_1646 0.17 -0.4 1,662,760 - EX28DRAFT_1646 0.17 +2.0 1,662,760 - EX28DRAFT_1646 0.17 -0.9 1,662,760 - EX28DRAFT_1646 0.17 +0.1 1,662,760 - EX28DRAFT_1646 0.17 +0.1 1,662,766 - EX28DRAFT_1646 0.17 -0.4 1,662,766 - EX28DRAFT_1646 0.17 +0.1 1,663,005 - EX28DRAFT_1646 0.32 -0.4 1,663,012 - EX28DRAFT_1646 0.32 -0.5 1,663,068 + EX28DRAFT_1646 0.36 -0.2 1,663,069 - EX28DRAFT_1646 0.36 -0.3 1,663,069 - EX28DRAFT_1646 0.36 -2.2 1,663,089 + EX28DRAFT_1646 0.37 +0.5 1,663,089 + EX28DRAFT_1646 0.37 -0.0 1,663,089 + EX28DRAFT_1646 0.37 +0.3 1,663,231 - EX28DRAFT_1646 0.46 -0.0 1,663,290 - EX28DRAFT_1646 0.49 -1.6 1,663,372 - EX28DRAFT_1646 0.54 +0.2 1,663,406 - EX28DRAFT_1646 0.56 -0.0 1,663,467 - EX28DRAFT_1646 0.60 -0.2 1,663,563 + EX28DRAFT_1646 0.66 -0.1 1,663,601 + EX28DRAFT_1646 0.68 -0.1 1,663,644 + EX28DRAFT_1646 0.71 -0.4 1,663,645 - EX28DRAFT_1646 0.71 -0.3 1,663,673 + EX28DRAFT_1646 0.73 -0.2 1,663,736 + EX28DRAFT_1646 0.77 +0.1 1,663,736 + EX28DRAFT_1646 0.77 -1.9 1,663,737 - EX28DRAFT_1646 0.77 -0.3 1,663,737 - EX28DRAFT_1646 0.77 -0.6 1,663,740 + EX28DRAFT_1646 0.77 -1.0 1,663,741 - EX28DRAFT_1646 0.77 +0.0 1,663,741 - EX28DRAFT_1646 0.77 -0.5 1,663,759 - EX28DRAFT_1646 0.78 -0.6 1,663,759 - EX28DRAFT_1646 0.78 +0.0 1,663,871 + EX28DRAFT_1646 0.85 +0.3 1,663,875 + EX28DRAFT_1646 0.85 +1.2 1,663,923 - EX28DRAFT_1646 0.88 -0.4 1,663,932 - EX28DRAFT_1646 0.89 -0.6 1,664,030 - -0.5 1,664,091 - +0.2 1,664,092 + +0.6 1,664,092 + +0.9 1,664,093 - +0.7 1,664,093 - -0.4 1,664,093 - -2.4 1,664,094 - -0.4 1,664,167 + -0.0 1,664,297 + EX28DRAFT_1647 0.23 +0.3 1,664,395 + EX28DRAFT_1647 0.40 -0.8 1,664,395 + EX28DRAFT_1647 0.40 -1.5 1,664,420 + EX28DRAFT_1647 0.44 -1.2 1,664,519 - EX28DRAFT_1647 0.60 -0.2 1,664,536 + EX28DRAFT_1647 0.63 +0.3 1,664,643 + EX28DRAFT_1647 0.81 -0.2 1,664,644 - EX28DRAFT_1647 0.81 -0.1 1,664,645 + EX28DRAFT_1647 0.81 -0.2 1,664,646 - EX28DRAFT_1647 0.82 -0.4 1,664,665 + EX28DRAFT_1647 0.85 -0.0 1,664,738 - -0.7 1,664,738 - -0.4 1,664,754 - +0.2 1,664,754 - +0.3 1,664,766 + +0.9 1,664,770 + +1.1 1,664,808 + +0.3 1,664,809 - -1.2 1,664,830 - +1.2 1,664,871 - +0.6 1,664,871 - +0.4 1,664,874 + +0.3 1,664,941 + EX28DRAFT_1648 0.12 +0.5 1,664,941 + EX28DRAFT_1648 0.12 +0.3 1,664,941 + EX28DRAFT_1648 0.12 -0.7 1,664,941 + EX28DRAFT_1648 0.12 +0.1 1,664,942 - EX28DRAFT_1648 0.12 +0.4
Or see this region's nucleotide sequence