Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1621 and EX28DRAFT_1622 are separated by 86 nucleotides EX28DRAFT_1622 and EX28DRAFT_1623 are separated by 76 nucleotides EX28DRAFT_1623 and EX28DRAFT_1624 overlap by 1 nucleotides EX28DRAFT_1624 and EX28DRAFT_1625 overlap by 8 nucleotides
EX28DRAFT_1621: EX28DRAFT_1621 - Haemolysin expression modulating protein, at 1,639,938 to 1,640,153
_1621
EX28DRAFT_1622: EX28DRAFT_1622 - Protein of unknown function (DUF2569), at 1,640,240 to 1,640,680
_1622
EX28DRAFT_1623: EX28DRAFT_1623 - electron transport complex, RnfABCDGE type, A subunit, at 1,640,757 to 1,641,338
_1623
EX28DRAFT_1624: EX28DRAFT_1624 - electron transport complex, RnfABCDGE type, B subunit, at 1,641,338 to 1,641,916
_1624
EX28DRAFT_1625: EX28DRAFT_1625 - electron transport complex, RnfABCDGE type, C subunit, at 1,641,909 to 1,644,044
_1625
Position (kb)
1640
1641
1642 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1639.907 kb on - strand at 1639.907 kb on - strand at 1639.907 kb on - strand at 1639.909 kb on + strand at 1639.920 kb on - strand at 1639.934 kb on + strand at 1639.937 kb on + strand at 1639.938 kb on - strand at 1639.938 kb on - strand at 1640.019 kb on + strand, within EX28DRAFT_1621 at 1640.020 kb on - strand, within EX28DRAFT_1621 at 1640.020 kb on - strand, within EX28DRAFT_1621 at 1640.048 kb on + strand, within EX28DRAFT_1621 at 1640.049 kb on - strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.051 kb on + strand, within EX28DRAFT_1621 at 1640.055 kb on + strand, within EX28DRAFT_1621 at 1640.127 kb on - strand, within EX28DRAFT_1621 at 1640.150 kb on - strand at 1640.151 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.181 kb on + strand at 1640.182 kb on - strand at 1640.182 kb on - strand at 1640.183 kb on + strand at 1640.183 kb on + strand at 1640.183 kb on + strand at 1640.184 kb on - strand at 1640.184 kb on - strand at 1640.228 kb on - strand at 1640.311 kb on - strand, within EX28DRAFT_1622 at 1640.340 kb on - strand, within EX28DRAFT_1622 at 1640.401 kb on + strand, within EX28DRAFT_1622 at 1640.450 kb on + strand, within EX28DRAFT_1622 at 1640.480 kb on - strand, within EX28DRAFT_1622 at 1640.500 kb on + strand, within EX28DRAFT_1622 at 1640.507 kb on + strand, within EX28DRAFT_1622 at 1640.508 kb on - strand, within EX28DRAFT_1622 at 1640.508 kb on - strand, within EX28DRAFT_1622 at 1640.508 kb on - strand, within EX28DRAFT_1622 at 1640.513 kb on - strand, within EX28DRAFT_1622 at 1640.679 kb on - strand at 1640.694 kb on - strand at 1640.709 kb on - strand at 1640.728 kb on + strand at 1640.729 kb on - strand at 1640.755 kb on + strand at 1640.756 kb on - strand at 1640.770 kb on + strand at 1640.771 kb on - strand at 1640.771 kb on - strand at 1640.967 kb on + strand, within EX28DRAFT_1623 at 1640.988 kb on - strand, within EX28DRAFT_1623 at 1641.018 kb on - strand, within EX28DRAFT_1623 at 1641.197 kb on + strand, within EX28DRAFT_1623 at 1641.198 kb on - strand, within EX28DRAFT_1623 at 1641.318 kb on - strand at 1641.820 kb on + strand, within EX28DRAFT_1624 at 1641.820 kb on + strand, within EX28DRAFT_1624 at 1641.914 kb on + strand at 1641.914 kb on + strand at 1641.914 kb on + strand at 1641.914 kb on + strand at 1641.915 kb on - strand at 1641.915 kb on - strand at 1641.915 kb on - strand at 1641.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,639,907 - +1.4 1,639,907 - -0.1 1,639,907 - +0.7 1,639,909 + +0.9 1,639,920 - -2.1 1,639,934 + -0.6 1,639,937 + -0.1 1,639,938 - -0.1 1,639,938 - -0.3 1,640,019 + EX28DRAFT_1621 0.38 -1.3 1,640,020 - EX28DRAFT_1621 0.38 -0.8 1,640,020 - EX28DRAFT_1621 0.38 -0.6 1,640,048 + EX28DRAFT_1621 0.51 -0.8 1,640,049 - EX28DRAFT_1621 0.51 -0.6 1,640,051 + EX28DRAFT_1621 0.52 -1.1 1,640,051 + EX28DRAFT_1621 0.52 -0.9 1,640,051 + EX28DRAFT_1621 0.52 -0.5 1,640,055 + EX28DRAFT_1621 0.54 -0.8 1,640,127 - EX28DRAFT_1621 0.88 -0.1 1,640,150 - -0.2 1,640,151 + -0.4 1,640,181 + +0.0 1,640,181 + -1.2 1,640,181 + -0.2 1,640,181 + +0.4 1,640,181 + +0.2 1,640,182 - +0.3 1,640,182 - -0.2 1,640,183 + +0.3 1,640,183 + -0.6 1,640,183 + +1.6 1,640,184 - -0.0 1,640,184 - +0.2 1,640,228 - +0.4 1,640,311 - EX28DRAFT_1622 0.16 +0.9 1,640,340 - EX28DRAFT_1622 0.23 -0.0 1,640,401 + EX28DRAFT_1622 0.37 -0.1 1,640,450 + EX28DRAFT_1622 0.48 +0.1 1,640,480 - EX28DRAFT_1622 0.54 +0.2 1,640,500 + EX28DRAFT_1622 0.59 -2.8 1,640,507 + EX28DRAFT_1622 0.61 -0.4 1,640,508 - EX28DRAFT_1622 0.61 -0.2 1,640,508 - EX28DRAFT_1622 0.61 -0.1 1,640,508 - EX28DRAFT_1622 0.61 +1.0 1,640,513 - EX28DRAFT_1622 0.62 +0.6 1,640,679 - -0.5 1,640,694 - -0.2 1,640,709 - -1.6 1,640,728 + -1.5 1,640,729 - -0.8 1,640,755 + -0.8 1,640,756 - -1.1 1,640,770 + -2.7 1,640,771 - -0.4 1,640,771 - -0.7 1,640,967 + EX28DRAFT_1623 0.36 -2.1 1,640,988 - EX28DRAFT_1623 0.40 -0.1 1,641,018 - EX28DRAFT_1623 0.45 -1.3 1,641,197 + EX28DRAFT_1623 0.76 -1.1 1,641,198 - EX28DRAFT_1623 0.76 -0.7 1,641,318 - -2.0 1,641,820 + EX28DRAFT_1624 0.83 -0.4 1,641,820 + EX28DRAFT_1624 0.83 -0.9 1,641,914 + -0.3 1,641,914 + +0.3 1,641,914 + -1.3 1,641,914 + -1.2 1,641,915 - -1.6 1,641,915 - -0.8 1,641,915 - -1.5 1,641,915 - -2.0
Or see this region's nucleotide sequence