Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1547 and EX28DRAFT_1548 overlap by 4 nucleotides EX28DRAFT_1548 and EX28DRAFT_1549 are separated by 92 nucleotides EX28DRAFT_1549 and EX28DRAFT_1550 are separated by 253 nucleotides EX28DRAFT_1550 and EX28DRAFT_1551 are separated by 450 nucleotides
EX28DRAFT_1547: EX28DRAFT_1547 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,563,644 to 1,564,366
_1547
EX28DRAFT_1548: EX28DRAFT_1548 - Uncharacterized membrane protein required for alginate biosynthesis, at 1,564,363 to 1,564,698
_1548
EX28DRAFT_1549: EX28DRAFT_1549 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,564,791 to 1,565,543
_1549
EX28DRAFT_1550: EX28DRAFT_1550 - hypothetical protein, at 1,565,797 to 1,566,057
_1550
EX28DRAFT_1551: EX28DRAFT_1551 - hypothetical protein, at 1,566,508 to 1,566,963
_1551
Position (kb)
1564
1565
1566 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1564.046 kb on - strand, within EX28DRAFT_1547 at 1564.298 kb on + strand at 1564.299 kb on - strand at 1564.316 kb on + strand at 1564.316 kb on + strand at 1564.391 kb on - strand at 1564.484 kb on + strand, within EX28DRAFT_1548 at 1564.503 kb on + strand, within EX28DRAFT_1548 at 1564.530 kb on + strand, within EX28DRAFT_1548 at 1564.566 kb on + strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.567 kb on - strand, within EX28DRAFT_1548 at 1564.568 kb on + strand, within EX28DRAFT_1548 at 1564.568 kb on + strand, within EX28DRAFT_1548 at 1564.569 kb on - strand, within EX28DRAFT_1548 at 1564.618 kb on - strand, within EX28DRAFT_1548 at 1564.619 kb on + strand, within EX28DRAFT_1548 at 1564.619 kb on + strand, within EX28DRAFT_1548 at 1564.655 kb on - strand, within EX28DRAFT_1548 at 1564.655 kb on - strand, within EX28DRAFT_1548 at 1564.683 kb on + strand at 1564.728 kb on - strand at 1564.794 kb on + strand at 1564.794 kb on + strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.795 kb on - strand at 1564.826 kb on + strand at 1564.827 kb on - strand at 1564.842 kb on + strand at 1564.843 kb on - strand at 1564.901 kb on + strand, within EX28DRAFT_1549 at 1565.048 kb on + strand, within EX28DRAFT_1549 at 1565.118 kb on + strand, within EX28DRAFT_1549 at 1565.119 kb on - strand, within EX28DRAFT_1549 at 1565.648 kb on - strand at 1565.672 kb on - strand at 1565.672 kb on - strand at 1565.783 kb on + strand at 1565.784 kb on - strand at 1565.800 kb on + strand at 1565.800 kb on + strand at 1565.800 kb on + strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.801 kb on - strand at 1565.803 kb on - strand at 1565.807 kb on - strand at 1565.807 kb on - strand at 1565.807 kb on - strand at 1565.816 kb on + strand at 1565.816 kb on + strand at 1565.952 kb on - strand, within EX28DRAFT_1550 at 1565.952 kb on - strand, within EX28DRAFT_1550 at 1565.952 kb on - strand, within EX28DRAFT_1550 at 1565.980 kb on + strand, within EX28DRAFT_1550 at 1565.982 kb on + strand, within EX28DRAFT_1550 at 1566.055 kb on + strand at 1566.056 kb on - strand at 1566.097 kb on + strand at 1566.097 kb on + strand at 1566.097 kb on + strand at 1566.098 kb on - strand at 1566.098 kb on - strand at 1566.099 kb on + strand at 1566.398 kb on + strand at 1566.398 kb on + strand at 1566.399 kb on - strand at 1566.399 kb on - strand at 1566.418 kb on + strand at 1566.418 kb on + strand at 1566.425 kb on + strand at 1566.472 kb on + strand at 1566.472 kb on + strand at 1566.518 kb on + strand at 1566.519 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,564,046 - EX28DRAFT_1547 0.56 -0.2 1,564,298 + -0.8 1,564,299 - -0.6 1,564,316 + +0.4 1,564,316 + -0.8 1,564,391 - -0.3 1,564,484 + EX28DRAFT_1548 0.36 +0.3 1,564,503 + EX28DRAFT_1548 0.42 +0.3 1,564,530 + EX28DRAFT_1548 0.50 -0.0 1,564,566 + EX28DRAFT_1548 0.60 -1.0 1,564,567 - EX28DRAFT_1548 0.61 -0.5 1,564,567 - EX28DRAFT_1548 0.61 -2.1 1,564,567 - EX28DRAFT_1548 0.61 +0.4 1,564,567 - EX28DRAFT_1548 0.61 +0.7 1,564,568 + EX28DRAFT_1548 0.61 -0.3 1,564,568 + EX28DRAFT_1548 0.61 +0.3 1,564,569 - EX28DRAFT_1548 0.61 -0.9 1,564,618 - EX28DRAFT_1548 0.76 +0.3 1,564,619 + EX28DRAFT_1548 0.76 -0.4 1,564,619 + EX28DRAFT_1548 0.76 +1.8 1,564,655 - EX28DRAFT_1548 0.87 +0.6 1,564,655 - EX28DRAFT_1548 0.87 -0.1 1,564,683 + +0.0 1,564,728 - -0.1 1,564,794 + -0.6 1,564,794 + -0.0 1,564,795 - +0.2 1,564,795 - -1.8 1,564,795 - -0.9 1,564,795 - -0.3 1,564,795 - -0.1 1,564,826 + -0.6 1,564,827 - +0.5 1,564,842 + -0.1 1,564,843 - +0.1 1,564,901 + EX28DRAFT_1549 0.15 +0.6 1,565,048 + EX28DRAFT_1549 0.34 +0.6 1,565,118 + EX28DRAFT_1549 0.43 +0.3 1,565,119 - EX28DRAFT_1549 0.44 +0.0 1,565,648 - -0.1 1,565,672 - +0.3 1,565,672 - -0.8 1,565,783 + -0.6 1,565,784 - +0.0 1,565,800 + +0.9 1,565,800 + -0.8 1,565,800 + -0.1 1,565,801 - +0.3 1,565,801 - +0.4 1,565,801 - -0.2 1,565,801 - +0.6 1,565,801 - +0.7 1,565,801 - -1.1 1,565,801 - -0.0 1,565,801 - +1.0 1,565,803 - -0.9 1,565,807 - -1.3 1,565,807 - -0.4 1,565,807 - +1.1 1,565,816 + -0.8 1,565,816 + -0.4 1,565,952 - EX28DRAFT_1550 0.59 +0.4 1,565,952 - EX28DRAFT_1550 0.59 +0.5 1,565,952 - EX28DRAFT_1550 0.59 -0.1 1,565,980 + EX28DRAFT_1550 0.70 +0.5 1,565,982 + EX28DRAFT_1550 0.71 -0.2 1,566,055 + -0.9 1,566,056 - -0.1 1,566,097 + -0.7 1,566,097 + -0.5 1,566,097 + +0.3 1,566,098 - -1.1 1,566,098 - +0.5 1,566,099 + -0.0 1,566,398 + +0.7 1,566,398 + -1.2 1,566,399 - +1.4 1,566,399 - +2.1 1,566,418 + +0.5 1,566,418 + +0.0 1,566,425 + +0.1 1,566,472 + -0.1 1,566,472 + -0.4 1,566,518 + -1.8 1,566,519 - -1.1
Or see this region's nucleotide sequence