Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1028 and EX28DRAFT_1029 overlap by 11 nucleotides EX28DRAFT_1029 and EX28DRAFT_1030 are separated by 24 nucleotides EX28DRAFT_1030 and EX28DRAFT_1031 are separated by 183 nucleotides EX28DRAFT_1031 and EX28DRAFT_1032 are separated by 85 nucleotides
EX28DRAFT_1028: EX28DRAFT_1028 - ABC-type spermidine/putrescine transport system, permease component I, at 1,035,379 to 1,036,323
_1028
EX28DRAFT_1029: EX28DRAFT_1029 - ABC-type spermidine/putrescine transport system, permease component II, at 1,036,313 to 1,037,119
_1029
EX28DRAFT_1030: EX28DRAFT_1030 - 1-pyrroline dehydrogenase, at 1,037,144 to 1,038,568
_1030
EX28DRAFT_1031: EX28DRAFT_1031 - Protein of unknown function (DUF2566), at 1,038,752 to 1,038,925
_1031
EX28DRAFT_1032: EX28DRAFT_1032 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 1,039,011 to 1,039,766
_1032
Position (kb)
1037
1038
1039 Strain fitness (log2 ratio)
-2
-1
0
1 at 1036.228 kb on - strand, within EX28DRAFT_1028 at 1036.279 kb on + strand at 1036.404 kb on - strand, within EX28DRAFT_1029 at 1036.719 kb on + strand, within EX28DRAFT_1029 at 1036.760 kb on - strand, within EX28DRAFT_1029 at 1036.926 kb on + strand, within EX28DRAFT_1029 at 1037.118 kb on - strand at 1037.143 kb on + strand at 1037.207 kb on + strand at 1037.207 kb on + strand at 1037.519 kb on + strand, within EX28DRAFT_1030 at 1037.519 kb on + strand, within EX28DRAFT_1030 at 1037.576 kb on - strand, within EX28DRAFT_1030 at 1037.591 kb on + strand, within EX28DRAFT_1030 at 1037.713 kb on + strand, within EX28DRAFT_1030 at 1037.713 kb on + strand, within EX28DRAFT_1030 at 1037.797 kb on + strand, within EX28DRAFT_1030 at 1037.798 kb on - strand, within EX28DRAFT_1030 at 1037.835 kb on + strand, within EX28DRAFT_1030 at 1037.837 kb on - strand, within EX28DRAFT_1030 at 1037.963 kb on + strand, within EX28DRAFT_1030 at 1037.964 kb on - strand, within EX28DRAFT_1030 at 1038.002 kb on + strand, within EX28DRAFT_1030 at 1038.021 kb on - strand, within EX28DRAFT_1030 at 1038.172 kb on + strand, within EX28DRAFT_1030 at 1038.219 kb on - strand, within EX28DRAFT_1030 at 1038.219 kb on - strand, within EX28DRAFT_1030 at 1038.219 kb on - strand, within EX28DRAFT_1030 at 1038.422 kb on + strand, within EX28DRAFT_1030 at 1038.508 kb on + strand at 1038.508 kb on + strand at 1038.509 kb on - strand at 1038.533 kb on + strand at 1038.567 kb on - strand at 1038.567 kb on - strand at 1038.663 kb on - strand at 1038.697 kb on + strand at 1038.705 kb on + strand at 1038.747 kb on - strand at 1038.790 kb on + strand, within EX28DRAFT_1031 at 1038.814 kb on + strand, within EX28DRAFT_1031 at 1038.979 kb on + strand at 1038.980 kb on - strand at 1039.010 kb on + strand at 1039.011 kb on - strand at 1039.126 kb on - strand, within EX28DRAFT_1032 at 1039.197 kb on + strand, within EX28DRAFT_1032 at 1039.218 kb on + strand, within EX28DRAFT_1032 at 1039.218 kb on + strand, within EX28DRAFT_1032 at 1039.218 kb on + strand, within EX28DRAFT_1032 at 1039.218 kb on + strand, within EX28DRAFT_1032 at 1039.218 kb on + strand, within EX28DRAFT_1032 at 1039.224 kb on + strand, within EX28DRAFT_1032 at 1039.225 kb on - strand, within EX28DRAFT_1032 at 1039.227 kb on - strand, within EX28DRAFT_1032 at 1039.296 kb on + strand, within EX28DRAFT_1032 at 1039.418 kb on + strand, within EX28DRAFT_1032 at 1039.419 kb on - strand, within EX28DRAFT_1032 at 1039.522 kb on - strand, within EX28DRAFT_1032
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,036,228 - EX28DRAFT_1028 0.90 +0.1 1,036,279 + -0.4 1,036,404 - EX28DRAFT_1029 0.11 -0.5 1,036,719 + EX28DRAFT_1029 0.50 -0.2 1,036,760 - EX28DRAFT_1029 0.55 -2.2 1,036,926 + EX28DRAFT_1029 0.76 -0.7 1,037,118 - -0.8 1,037,143 + -0.4 1,037,207 + +0.1 1,037,207 + +1.5 1,037,519 + EX28DRAFT_1030 0.26 +0.3 1,037,519 + EX28DRAFT_1030 0.26 -0.2 1,037,576 - EX28DRAFT_1030 0.30 +0.5 1,037,591 + EX28DRAFT_1030 0.31 +1.3 1,037,713 + EX28DRAFT_1030 0.40 +0.2 1,037,713 + EX28DRAFT_1030 0.40 -2.2 1,037,797 + EX28DRAFT_1030 0.46 +0.2 1,037,798 - EX28DRAFT_1030 0.46 -0.4 1,037,835 + EX28DRAFT_1030 0.48 -0.4 1,037,837 - EX28DRAFT_1030 0.49 +1.4 1,037,963 + EX28DRAFT_1030 0.57 -0.5 1,037,964 - EX28DRAFT_1030 0.58 +1.1 1,038,002 + EX28DRAFT_1030 0.60 +0.3 1,038,021 - EX28DRAFT_1030 0.62 -1.2 1,038,172 + EX28DRAFT_1030 0.72 -0.1 1,038,219 - EX28DRAFT_1030 0.75 +0.1 1,038,219 - EX28DRAFT_1030 0.75 -0.2 1,038,219 - EX28DRAFT_1030 0.75 -0.2 1,038,422 + EX28DRAFT_1030 0.90 -0.0 1,038,508 + +0.8 1,038,508 + +0.1 1,038,509 - -1.1 1,038,533 + +0.2 1,038,567 - +0.7 1,038,567 - -0.2 1,038,663 - +0.6 1,038,697 + +0.4 1,038,705 + +1.1 1,038,747 - +0.1 1,038,790 + EX28DRAFT_1031 0.22 -0.6 1,038,814 + EX28DRAFT_1031 0.36 +0.5 1,038,979 + -0.7 1,038,980 - -0.1 1,039,010 + -1.7 1,039,011 - +0.4 1,039,126 - EX28DRAFT_1032 0.15 -0.4 1,039,197 + EX28DRAFT_1032 0.25 -0.7 1,039,218 + EX28DRAFT_1032 0.27 +0.4 1,039,218 + EX28DRAFT_1032 0.27 -0.1 1,039,218 + EX28DRAFT_1032 0.27 -0.9 1,039,218 + EX28DRAFT_1032 0.27 -0.4 1,039,218 + EX28DRAFT_1032 0.27 +0.4 1,039,224 + EX28DRAFT_1032 0.28 -1.0 1,039,225 - EX28DRAFT_1032 0.28 -0.3 1,039,227 - EX28DRAFT_1032 0.29 +0.5 1,039,296 + EX28DRAFT_1032 0.38 -0.4 1,039,418 + EX28DRAFT_1032 0.54 -0.1 1,039,419 - EX28DRAFT_1032 0.54 +0.2 1,039,522 - EX28DRAFT_1032 0.68 -0.7
Or see this region's nucleotide sequence