Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0526 and EX28DRAFT_0527 are separated by 46 nucleotides EX28DRAFT_0527 and EX28DRAFT_0528 are separated by 58 nucleotides EX28DRAFT_0528 and EX28DRAFT_0529 are separated by 146 nucleotides
EX28DRAFT_0526: EX28DRAFT_0526 - hypothetical protein, at 519,418 to 520,428
_0526
EX28DRAFT_0527: EX28DRAFT_0527 - glucose-1-phosphate thymidylyltransferase, short form, at 520,475 to 521,347
_0527
EX28DRAFT_0528: EX28DRAFT_0528 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 521,406 to 522,872
_0528
EX28DRAFT_0529: EX28DRAFT_0529 - 6-phosphogluconate dehydrogenase (decarboxylating), at 523,019 to 524,425
_0529
Position (kb)
521
522
523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 520.460 kb on + strand at 521.319 kb on + strand at 521.593 kb on - strand, within EX28DRAFT_0528 at 522.123 kb on + strand, within EX28DRAFT_0528 at 522.743 kb on + strand at 522.810 kb on + strand at 522.958 kb on + strand at 522.970 kb on - strand at 523.013 kb on + strand at 523.017 kb on + strand at 523.017 kb on + strand at 523.100 kb on - strand at 523.533 kb on - strand, within EX28DRAFT_0529 at 523.560 kb on - strand, within EX28DRAFT_0529
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 520,460 + +0.6 521,319 + -0.9 521,593 - EX28DRAFT_0528 0.13 +0.8 522,123 + EX28DRAFT_0528 0.49 +1.9 522,743 + -1.1 522,810 + +0.1 522,958 + -0.3 522,970 - -0.0 523,013 + -1.3 523,017 + +0.2 523,017 + -0.8 523,100 - -0.9 523,533 - EX28DRAFT_0529 0.37 -1.0 523,560 - EX28DRAFT_0529 0.38 -2.0
Or see this region's nucleotide sequence