Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0321 and EX28DRAFT_0322 are separated by 15 nucleotides EX28DRAFT_0322 and EX28DRAFT_0323 are separated by 89 nucleotides EX28DRAFT_0323 and EX28DRAFT_0324 are separated by 2 nucleotides EX28DRAFT_0324 and EX28DRAFT_0325 overlap by 4 nucleotides
EX28DRAFT_0321: EX28DRAFT_0321 - NADH:ubiquinone oxidoreductase subunit 3 (chain A), at 282,618 to 283,058
_0321
EX28DRAFT_0322: EX28DRAFT_0322 - NADH-quinone oxidoreductase, B subunit, at 283,074 to 283,748
_0322
EX28DRAFT_0323: EX28DRAFT_0323 - NADH (or F420H2) dehydrogenase, subunit C, at 283,838 to 285,640
_0323
EX28DRAFT_0324: EX28DRAFT_0324 - NADH-quinone oxidoreductase, E subunit, at 285,643 to 286,143
_0324
EX28DRAFT_0325: EX28DRAFT_0325 - NADH-quinone oxidoreductase, F subunit, at 286,140 to 287,477
_0325
Position (kb)
283
284
285
286 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 283.037 kb on + strand at 283.082 kb on + strand at 283.186 kb on - strand, within EX28DRAFT_0322 at 283.236 kb on - strand, within EX28DRAFT_0322 at 283.487 kb on + strand, within EX28DRAFT_0322 at 283.491 kb on + strand, within EX28DRAFT_0322 at 283.500 kb on + strand, within EX28DRAFT_0322 at 283.501 kb on - strand, within EX28DRAFT_0322 at 283.529 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.579 kb on - strand, within EX28DRAFT_0322 at 283.709 kb on + strand at 283.710 kb on - strand at 283.760 kb on - strand at 283.782 kb on + strand at 284.151 kb on + strand, within EX28DRAFT_0323 at 284.247 kb on - strand, within EX28DRAFT_0323 at 284.639 kb on + strand, within EX28DRAFT_0323 at 284.640 kb on - strand, within EX28DRAFT_0323 at 284.802 kb on - strand, within EX28DRAFT_0323 at 285.018 kb on - strand, within EX28DRAFT_0323 at 285.228 kb on - strand, within EX28DRAFT_0323 at 285.228 kb on - strand, within EX28DRAFT_0323 at 285.464 kb on - strand at 285.467 kb on - strand at 285.605 kb on - strand at 285.619 kb on + strand at 285.809 kb on - strand, within EX28DRAFT_0324 at 285.868 kb on + strand, within EX28DRAFT_0324 at 286.047 kb on + strand, within EX28DRAFT_0324 at 286.047 kb on + strand, within EX28DRAFT_0324
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 283,037 + -1.4 283,082 + -0.4 283,186 - EX28DRAFT_0322 0.17 -3.1 283,236 - EX28DRAFT_0322 0.24 -3.9 283,487 + EX28DRAFT_0322 0.61 -2.1 283,491 + EX28DRAFT_0322 0.62 -2.3 283,500 + EX28DRAFT_0322 0.63 -1.5 283,501 - EX28DRAFT_0322 0.63 -2.0 283,529 + EX28DRAFT_0322 0.67 -2.0 283,545 + EX28DRAFT_0322 0.70 -2.2 283,545 + EX28DRAFT_0322 0.70 -2.5 283,545 + EX28DRAFT_0322 0.70 -2.3 283,545 + EX28DRAFT_0322 0.70 -2.3 283,579 - EX28DRAFT_0322 0.75 -1.6 283,709 + -0.9 283,710 - -2.2 283,760 - -1.4 283,782 + -3.2 284,151 + EX28DRAFT_0323 0.17 -1.8 284,247 - EX28DRAFT_0323 0.23 -2.5 284,639 + EX28DRAFT_0323 0.44 -3.8 284,640 - EX28DRAFT_0323 0.44 -2.0 284,802 - EX28DRAFT_0323 0.53 +0.2 285,018 - EX28DRAFT_0323 0.65 -0.6 285,228 - EX28DRAFT_0323 0.77 -2.1 285,228 - EX28DRAFT_0323 0.77 -3.1 285,464 - -1.4 285,467 - -1.3 285,605 - -2.6 285,619 + -1.7 285,809 - EX28DRAFT_0324 0.33 -0.7 285,868 + EX28DRAFT_0324 0.45 -2.0 286,047 + EX28DRAFT_0324 0.81 -1.3 286,047 + EX28DRAFT_0324 0.81 -2.1
Or see this region's nucleotide sequence