Strain Fitness in Variovorax sp. OAS795 around ABID97_RS28015

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABID97_RS28005 and ABID97_RS28010 overlap by 8 nucleotidesABID97_RS28010 and ABID97_RS28015 overlap by 8 nucleotidesABID97_RS28015 and ABID97_RS28020 overlap by 11 nucleotidesABID97_RS28020 and ABID97_RS28025 overlap by 11 nucleotides ABID97_RS28005: ABID97_RS28005 - phosphopyruvate hydratase, at 519,383 to 520,462 _RS28005 ABID97_RS28010: ABID97_RS28010 - hypothetical protein, at 520,455 to 520,676 _RS28010 ABID97_RS28015: ABID97_RS28015 - cupin domain-containing protein, at 520,669 to 521,280 _RS28015 ABID97_RS28020: ABID97_RS28020 - ABC transporter ATP-binding protein, at 521,270 to 521,977 _RS28020 ABID97_RS28025: ABID97_RS28025 - branched-chain amino acid ABC transporter ATP-binding protein/permease, at 521,967 to 523,736 _RS28025 Position (kb) 520 521 522Strain fitness (log2 ratio) -1 0 1at 519.916 kb on - strand, within ABID97_RS28005at 520.119 kb on + strand, within ABID97_RS28005at 521.361 kb on + strand, within ABID97_RS28020at 521.362 kb on - strand, within ABID97_RS28020at 521.964 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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519,916 - ABID97_RS28005 0.49 +0.5
520,119 + ABID97_RS28005 0.68 -0.2
521,361 + ABID97_RS28020 0.13 +0.5
521,362 - ABID97_RS28020 0.13 +0.3
521,964 - +0.1

Or see this region's nucleotide sequence