Strain Fitness in Variovorax sp. OAS795 around ABID97_RS27450

Experiment: L-Leucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABID97_RS27440 and ABID97_RS27445 overlap by 4 nucleotidesABID97_RS27445 and ABID97_RS27450 are separated by 26 nucleotidesABID97_RS27450 and ABID97_RS27455 are separated by 18 nucleotidesABID97_RS27455 and ABID97_RS27460 overlap by 4 nucleotides ABID97_RS27440: ABID97_RS27440 - triphosphoribosyl-dephospho-CoA synthase, at 405,780 to 406,643 _RS27440 ABID97_RS27445: ABID97_RS27445 - malonate decarboxylase holo-[acyl-carrier-protein] synthase, at 406,640 to 407,320 _RS27445 ABID97_RS27450: ABID97_RS27450 - biotin-independent malonate decarboxylase subunit gamma, at 407,347 to 408,048 _RS27450 ABID97_RS27455: ABID97_RS27455 - biotin-independent malonate decarboxylase subunit beta, at 408,067 to 408,948 _RS27455 ABID97_RS27460: ABID97_RS27460 - malonate decarboxylase acyl carrier protein, at 408,945 to 409,277 _RS27460 Position (kb) 407 408 409Strain fitness (log2 ratio) -1 0 1at 406.447 kb on + strand, within ABID97_RS27440at 406.561 kb on + strandat 406.561 kb on + strandat 406.913 kb on + strand, within ABID97_RS27445at 406.913 kb on + strand, within ABID97_RS27445at 407.646 kb on - strand, within ABID97_RS27450at 408.938 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (C)
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406,447 + ABID97_RS27440 0.77 +0.1
406,561 + +0.2
406,561 + -0.1
406,913 + ABID97_RS27445 0.40 +0.2
406,913 + ABID97_RS27445 0.40 +0.7
407,646 - ABID97_RS27450 0.43 -1.4
408,938 + -0.0

Or see this region's nucleotide sequence