Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS26290 and ABID97_RS26295 are separated by 279 nucleotides ABID97_RS26295 and ABID97_RS26300 are separated by 88 nucleotides ABID97_RS26300 and ABID97_RS26305 are separated by 30 nucleotides
ABID97_RS26290: ABID97_RS26290 - LysR substrate-binding domain-containing protein, at 181,973 to 182,860
_RS26290
ABID97_RS26295: ABID97_RS26295 - tripartite tricarboxylate transporter substrate binding protein, at 183,140 to 184,189
_RS26295
ABID97_RS26300: ABID97_RS26300 - response regulator, at 184,278 to 184,916
_RS26300
ABID97_RS26305: ABID97_RS26305 - response regulator, at 184,947 to 185,336
_RS26305
Position (kb)
183
184
185 Strain fitness (log2 ratio)
-2
-1
0
1 at 182.306 kb on - strand, within ABID97_RS26290 at 182.452 kb on - strand, within ABID97_RS26290 at 183.117 kb on - strand at 183.293 kb on + strand, within ABID97_RS26295 at 183.449 kb on + strand, within ABID97_RS26295 at 183.477 kb on - strand, within ABID97_RS26295 at 184.297 kb on + strand at 184.298 kb on - strand at 184.302 kb on + strand at 184.303 kb on - strand at 184.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 182,306 - ABID97_RS26290 0.38 -0.4 182,452 - ABID97_RS26290 0.54 -1.9 183,117 - -0.3 183,293 + ABID97_RS26295 0.15 -0.1 183,449 + ABID97_RS26295 0.29 +1.1 183,477 - ABID97_RS26295 0.32 +0.5 184,297 + -0.2 184,298 - +1.3 184,302 + +0.6 184,303 - -0.3 184,915 - -0.4
Or see this region's nucleotide sequence