Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS23730 and ABID97_RS23735 overlap by 4 nucleotides ABID97_RS23735 and ABID97_RS23740 overlap by 4 nucleotides ABID97_RS23740 and ABID97_RS23745 are separated by 66 nucleotides ABID97_RS23745 and ABID97_RS23750 are separated by 171 nucleotides
ABID97_RS23730: ABID97_RS23730 - tetratricopeptide repeat protein, at 4,913,983 to 4,915,839
_RS23730
ABID97_RS23735: ABID97_RS23735 - lipoprotein insertase outer membrane protein LolB, at 4,915,836 to 4,916,360
_RS23735
ABID97_RS23740: ABID97_RS23740 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 4,916,357 to 4,917,247
_RS23740
ABID97_RS23745: ABID97_RS23745 - tRNA-Gln, at 4,917,314 to 4,917,390
_RS23745
ABID97_RS23750: ABID97_RS23750 - ribose-phosphate pyrophosphokinase, at 4,917,562 to 4,918,533
_RS23750
Position (kb)
4916
4917
4918 Strain fitness (log2 ratio)
-1
0
1 at 4915.498 kb on + strand, within ABID97_RS23730 at 4915.498 kb on + strand, within ABID97_RS23730 at 4915.499 kb on - strand, within ABID97_RS23730 at 4915.499 kb on - strand, within ABID97_RS23730 at 4915.675 kb on + strand at 4915.676 kb on - strand at 4915.676 kb on - strand at 4917.281 kb on + strand at 4917.281 kb on + strand at 4917.400 kb on + strand at 4917.400 kb on + strand at 4917.481 kb on + strand at 4917.530 kb on + strand at 4917.530 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 4,915,498 + ABID97_RS23730 0.82 -0.1 4,915,498 + ABID97_RS23730 0.82 +0.5 4,915,499 - ABID97_RS23730 0.82 -0.1 4,915,499 - ABID97_RS23730 0.82 -0.2 4,915,675 + +0.1 4,915,676 - -0.0 4,915,676 - -0.0 4,917,281 + +1.1 4,917,281 + -0.6 4,917,400 + -0.5 4,917,400 + -0.4 4,917,481 + -0.9 4,917,530 + -0.4 4,917,530 + +1.2
Or see this region's nucleotide sequence