Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS10875 and ABID97_RS10880 are separated by 136 nucleotides ABID97_RS10880 and ABID97_RS10885 are separated by 100 nucleotides ABID97_RS10885 and ABID97_RS10890 are separated by 147 nucleotides
ABID97_RS10875: ABID97_RS10875 - peroxiredoxin, at 2,252,737 to 2,253,213
_RS10875
ABID97_RS10880: ABID97_RS10880 - Mth938-like domain-containing protein, at 2,253,350 to 2,253,733
_RS10880
ABID97_RS10885: ABID97_RS10885 - pyridoxal phosphate-dependent aminotransferase, at 2,253,834 to 2,255,066
_RS10885
ABID97_RS10890: ABID97_RS10890 - homoserine dehydrogenase, at 2,255,214 to 2,256,536
_RS10890
Position (kb)
2253
2254
2255
2256 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2253.420 kb on + strand, within ABID97_RS10880 at 2253.420 kb on + strand, within ABID97_RS10880 at 2253.421 kb on - strand, within ABID97_RS10880 at 2253.421 kb on - strand, within ABID97_RS10880 at 2253.760 kb on + strand at 2254.128 kb on + strand, within ABID97_RS10885 at 2254.461 kb on + strand, within ABID97_RS10885 at 2254.476 kb on + strand, within ABID97_RS10885 at 2254.557 kb on + strand, within ABID97_RS10885 at 2254.830 kb on + strand, within ABID97_RS10885 at 2254.830 kb on + strand, within ABID97_RS10885 at 2254.860 kb on + strand, within ABID97_RS10885 at 2255.231 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 2,253,420 + ABID97_RS10880 0.18 +1.6 2,253,420 + ABID97_RS10880 0.18 +2.8 2,253,421 - ABID97_RS10880 0.18 -0.2 2,253,421 - ABID97_RS10880 0.18 -0.6 2,253,760 + -0.8 2,254,128 + ABID97_RS10885 0.24 -0.3 2,254,461 + ABID97_RS10885 0.51 -2.0 2,254,476 + ABID97_RS10885 0.52 +0.1 2,254,557 + ABID97_RS10885 0.59 -1.3 2,254,830 + ABID97_RS10885 0.81 -1.5 2,254,830 + ABID97_RS10885 0.81 -2.4 2,254,860 + ABID97_RS10885 0.83 -2.0 2,255,231 - +1.1
Or see this region's nucleotide sequence