Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS07415 and ABID97_RS07420 are separated by 125 nucleotides ABID97_RS07420 and ABID97_RS07425 are separated by 20 nucleotides ABID97_RS07425 and ABID97_RS07430 overlap by 4 nucleotides ABID97_RS07430 and ABID97_RS07435 are separated by 11 nucleotides
ABID97_RS07415: ABID97_RS07415 - quinolinate synthase NadA, at 1,566,406 to 1,567,524
_RS07415
ABID97_RS07420: ABID97_RS07420 - 6-carboxytetrahydropterin synthase, at 1,567,650 to 1,568,012
_RS07420
ABID97_RS07425: ABID97_RS07425 - 7-carboxy-7-deazaguanine synthase, at 1,568,033 to 1,568,671
_RS07425
ABID97_RS07430: ABID97_RS07430 - pantoate--beta-alanine ligase, at 1,568,668 to 1,569,516
_RS07430
ABID97_RS07435: ABID97_RS07435 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,569,528 to 1,570,421
_RS07435
Position (kb)
1568
1569 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1567.912 kb on + strand, within ABID97_RS07420 at 1567.912 kb on + strand, within ABID97_RS07420 at 1567.912 kb on + strand, within ABID97_RS07420 at 1567.913 kb on - strand, within ABID97_RS07420 at 1567.913 kb on - strand, within ABID97_RS07420 at 1568.643 kb on + strand at 1568.643 kb on + strand at 1568.664 kb on + strand at 1568.664 kb on + strand at 1568.664 kb on + strand at 1568.665 kb on - strand at 1568.665 kb on - strand at 1568.665 kb on - strand at 1568.808 kb on - strand, within ABID97_RS07430 at 1568.887 kb on + strand, within ABID97_RS07430 at 1568.887 kb on + strand, within ABID97_RS07430 at 1568.888 kb on - strand, within ABID97_RS07430 at 1568.888 kb on - strand, within ABID97_RS07430 at 1568.888 kb on - strand, within ABID97_RS07430 at 1568.888 kb on - strand, within ABID97_RS07430 at 1568.888 kb on - strand, within ABID97_RS07430 at 1568.957 kb on + strand, within ABID97_RS07430 at 1569.075 kb on - strand, within ABID97_RS07430 at 1569.234 kb on - strand, within ABID97_RS07430 at 1569.318 kb on - strand, within ABID97_RS07430 at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.512 kb on + strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.513 kb on - strand at 1569.530 kb on - strand at 1569.583 kb on + strand at 1569.583 kb on + strand at 1569.584 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,567,912 + ABID97_RS07420 0.72 +0.4 1,567,912 + ABID97_RS07420 0.72 -0.9 1,567,912 + ABID97_RS07420 0.72 +0.4 1,567,913 - ABID97_RS07420 0.72 +0.7 1,567,913 - ABID97_RS07420 0.72 -0.1 1,568,643 + -0.2 1,568,643 + +0.1 1,568,664 + -0.2 1,568,664 + -0.4 1,568,664 + +0.5 1,568,665 - +0.5 1,568,665 - -0.8 1,568,665 - -0.3 1,568,808 - ABID97_RS07430 0.16 +0.4 1,568,887 + ABID97_RS07430 0.26 -5.3 1,568,887 + ABID97_RS07430 0.26 -4.2 1,568,888 - ABID97_RS07430 0.26 -2.5 1,568,888 - ABID97_RS07430 0.26 -5.4 1,568,888 - ABID97_RS07430 0.26 -4.7 1,568,888 - ABID97_RS07430 0.26 -4.9 1,568,888 - ABID97_RS07430 0.26 -3.4 1,568,957 + ABID97_RS07430 0.34 -3.3 1,569,075 - ABID97_RS07430 0.48 -4.6 1,569,234 - ABID97_RS07430 0.67 -4.3 1,569,318 - ABID97_RS07430 0.77 -0.6 1,569,512 + -4.8 1,569,512 + -3.9 1,569,512 + -3.8 1,569,512 + -4.5 1,569,512 + -2.1 1,569,512 + -3.6 1,569,512 + -3.7 1,569,512 + +0.2 1,569,512 + +0.4 1,569,512 + -3.0 1,569,512 + -3.7 1,569,512 + -5.2 1,569,512 + -3.5 1,569,512 + +0.7 1,569,513 - -3.7 1,569,513 - -0.6 1,569,513 - -4.3 1,569,513 - -3.7 1,569,513 - -4.2 1,569,513 - -1.3 1,569,513 - +0.9 1,569,513 - -4.9 1,569,513 - -3.4 1,569,513 - -0.3 1,569,513 - -3.9 1,569,530 - +1.7 1,569,583 + -4.6 1,569,583 + -4.3 1,569,584 - -2.6
Or see this region's nucleotide sequence