Strain Fitness in Escherichia coli BW25113 around b2913

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygfA and rygC are separated by 59 nucleotidesrygC and ibsC overlap by 99 nucleotidesibsC and serA are separated by 228 nucleotidesserA and rpiA are separated by 255 nucleotidesrpiA and yqfE are separated by 55 nucleotides b2912: ygfA - putative ligase (VIMSS), at 3,054,263 to 3,054,811 ygfA b4446: rygC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,054,871 to 3,055,010 rygC b4665: ibsC - toxic membrane protein (RefSeq), at 3,054,912 to 3,054,971 ibsC b2913: serA - D-3-phosphoglycerate dehydrogenase (NCBI), at 3,055,200 to 3,056,432 serA b2914: rpiA - ribose-5-phosphate isomerase A (NCBI), at 3,056,688 to 3,057,347 rpiA b2915: yqfE - hypothetical protein (RefSeq), at 3,057,403 to 3,057,723 yqfE Position (kb) 3055 3056 3057Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3054.348 kb on + strand, within ygfAat 3054.388 kb on - strand, within ygfAat 3054.480 kb on - strand, within ygfAat 3054.480 kb on - strand, within ygfAat 3054.524 kb on + strand, within ygfAat 3054.532 kb on - strand, within ygfAat 3054.578 kb on + strand, within ygfAat 3054.696 kb on - strand, within ygfAat 3054.704 kb on + strand, within ygfAat 3054.704 kb on + strand, within ygfAat 3054.711 kb on + strand, within ygfAat 3054.711 kb on + strand, within ygfAat 3054.788 kb on - strandat 3054.897 kb on + strand, within rygCat 3054.946 kb on - strandat 3054.946 kb on - strandat 3055.023 kb on - strandat 3055.023 kb on - strandat 3055.280 kb on - strandat 3055.352 kb on + strand, within serAat 3055.352 kb on + strand, within serAat 3055.437 kb on - strand, within serAat 3055.535 kb on - strand, within serAat 3055.611 kb on + strand, within serAat 3055.611 kb on + strand, within serAat 3055.642 kb on - strand, within serAat 3055.674 kb on - strand, within serAat 3055.678 kb on + strand, within serAat 3055.678 kb on + strand, within serAat 3055.678 kb on + strand, within serAat 3055.708 kb on + strand, within serAat 3055.745 kb on + strand, within serAat 3055.745 kb on + strand, within serAat 3055.749 kb on - strand, within serAat 3055.749 kb on - strand, within serAat 3055.802 kb on - strand, within serAat 3055.843 kb on - strand, within serAat 3055.850 kb on + strand, within serAat 3055.869 kb on - strand, within serAat 3056.013 kb on + strand, within serAat 3056.118 kb on + strand, within serAat 3056.118 kb on - strand, within serAat 3056.132 kb on - strand, within serAat 3056.132 kb on - strand, within serAat 3056.177 kb on + strand, within serAat 3056.177 kb on + strand, within serAat 3056.253 kb on - strand, within serAat 3056.345 kb on + strandat 3056.388 kb on - strandat 3056.487 kb on - strandat 3056.551 kb on - strandat 3056.578 kb on + strandat 3056.600 kb on - strandat 3056.617 kb on + strandat 3057.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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3,054,348 + ygfA b2912 0.15 +0.7
3,054,388 - ygfA b2912 0.23 +0.3
3,054,480 - ygfA b2912 0.40 -2.0
3,054,480 - ygfA b2912 0.40 -0.7
3,054,524 + ygfA b2912 0.48 -0.9
3,054,532 - ygfA b2912 0.49 -0.0
3,054,578 + ygfA b2912 0.57 +0.9
3,054,696 - ygfA b2912 0.79 -0.9
3,054,704 + ygfA b2912 0.80 -0.4
3,054,704 + ygfA b2912 0.80 +0.3
3,054,711 + ygfA b2912 0.82 -1.4
3,054,711 + ygfA b2912 0.82 +0.3
3,054,788 - -0.7
3,054,897 + rygC b4446 0.19 +0.5
3,054,946 - -0.2
3,054,946 - +0.1
3,055,023 - -1.3
3,055,023 - -0.4
3,055,280 - -2.0
3,055,352 + serA b2913 0.12 -1.6
3,055,352 + serA b2913 0.12 -1.6
3,055,437 - serA b2913 0.19 -3.3
3,055,535 - serA b2913 0.27 -4.2
3,055,611 + serA b2913 0.33 -1.4
3,055,611 + serA b2913 0.33 -1.3
3,055,642 - serA b2913 0.36 -2.3
3,055,674 - serA b2913 0.38 -2.2
3,055,678 + serA b2913 0.39 -2.0
3,055,678 + serA b2913 0.39 -1.9
3,055,678 + serA b2913 0.39 -2.1
3,055,708 + serA b2913 0.41 -2.2
3,055,745 + serA b2913 0.44 -2.4
3,055,745 + serA b2913 0.44 -1.1
3,055,749 - serA b2913 0.45 -2.2
3,055,749 - serA b2913 0.45 -1.8
3,055,802 - serA b2913 0.49 -3.1
3,055,843 - serA b2913 0.52 -2.9
3,055,850 + serA b2913 0.53 -2.0
3,055,869 - serA b2913 0.54 -3.3
3,056,013 + serA b2913 0.66 -4.0
3,056,118 + serA b2913 0.74 -2.9
3,056,118 - serA b2913 0.74 -2.5
3,056,132 - serA b2913 0.76 -1.9
3,056,132 - serA b2913 0.76 -2.6
3,056,177 + serA b2913 0.79 -2.1
3,056,177 + serA b2913 0.79 -1.6
3,056,253 - serA b2913 0.85 -2.7
3,056,345 + -1.9
3,056,388 - -3.8
3,056,487 - +1.0
3,056,551 - -3.0
3,056,578 + -2.2
3,056,600 - -2.6
3,056,617 + -1.7
3,057,409 - -0.3

Or see this region's nucleotide sequence