Strain Fitness in Escherichia coli BW25113 around b2388

Experiment: succinate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntypdG and ypdH are separated by 21 nucleotidesypdH and glk are separated by 218 nucleotidesglk and yfeO are separated by 203 nucleotides b2386: ypdG - predicted enzyme IIC component of PTS (NCBI), at 2,504,669 to 2,505,916 ypdG b2387: ypdH - predicted enzyme IIB component of PTS (NCBI), at 2,505,938 to 2,506,264 ypdH b2388: glk - glucokinase (NCBI), at 2,506,483 to 2,507,448 glk b2389: yfeO - hypothetical protein (NCBI), at 2,507,652 to 2,508,908 yfeO Position (kb) 2506 2507 2508Strain fitness (log2 ratio) -3 -2 -1 0 1at 2505.504 kb on - strand, within ypdGat 2505.639 kb on + strand, within ypdGat 2505.648 kb on - strand, within ypdGat 2505.648 kb on - strand, within ypdGat 2505.672 kb on + strand, within ypdGat 2505.719 kb on + strand, within ypdGat 2505.719 kb on + strand, within ypdGat 2505.787 kb on + strand, within ypdGat 2505.885 kb on - strandat 2505.919 kb on + strandat 2505.927 kb on - strandat 2505.927 kb on - strandat 2505.949 kb on + strandat 2505.954 kb on - strandat 2505.954 kb on - strandat 2506.086 kb on - strand, within ypdHat 2506.086 kb on - strand, within ypdHat 2506.096 kb on + strand, within ypdHat 2506.129 kb on - strand, within ypdHat 2506.201 kb on - strand, within ypdHat 2506.212 kb on - strand, within ypdHat 2506.219 kb on - strand, within ypdHat 2506.225 kb on - strand, within ypdHat 2506.225 kb on - strand, within ypdHat 2506.286 kb on + strandat 2506.294 kb on - strandat 2506.370 kb on + strandat 2506.386 kb on - strandat 2506.393 kb on + strandat 2506.393 kb on + strandat 2506.428 kb on + strandat 2506.578 kb on - strandat 2506.646 kb on - strand, within glkat 2506.646 kb on - strand, within glkat 2506.731 kb on - strand, within glkat 2506.731 kb on - strand, within glkat 2506.780 kb on - strand, within glkat 2506.800 kb on - strand, within glkat 2506.861 kb on - strand, within glkat 2506.866 kb on + strand, within glkat 2507.004 kb on + strand, within glkat 2507.004 kb on + strand, within glkat 2507.022 kb on + strand, within glkat 2507.057 kb on + strand, within glkat 2507.185 kb on + strand, within glkat 2507.215 kb on + strand, within glkat 2507.333 kb on + strand, within glkat 2507.341 kb on - strand, within glkat 2507.341 kb on - strand, within glkat 2507.362 kb on - strandat 2507.362 kb on - strandat 2507.362 kb on - strandat 2507.392 kb on - strandat 2507.407 kb on - strandat 2507.448 kb on + strandat 2507.483 kb on + strandat 2507.493 kb on + strandat 2507.499 kb on - strandat 2507.506 kb on - strandat 2507.513 kb on - strandat 2507.540 kb on + strandat 2507.634 kb on - strandat 2507.634 kb on - strandat 2507.634 kb on - strandat 2507.803 kb on - strand, within yfeOat 2507.805 kb on + strand, within yfeOat 2507.835 kb on + strand, within yfeOat 2507.883 kb on - strand, within yfeOat 2507.883 kb on - strand, within yfeOat 2507.901 kb on + strand, within yfeOat 2507.904 kb on - strand, within yfeOat 2508.013 kb on + strand, within yfeOat 2508.302 kb on + strand, within yfeOat 2508.328 kb on + strand, within yfeOat 2508.368 kb on - strand, within yfeOat 2508.377 kb on + strand, within yfeOat 2508.377 kb on + strand, within yfeOat 2508.377 kb on + strand, within yfeO

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Per-strain Table

Position Strand Gene LocusTag Fraction succinate (C)
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2,505,504 - ypdG b2386 0.67 +0.5
2,505,639 + ypdG b2386 0.78 +0.8
2,505,648 - ypdG b2386 0.78 -2.0
2,505,648 - ypdG b2386 0.78 -1.6
2,505,672 + ypdG b2386 0.80 -0.5
2,505,719 + ypdG b2386 0.84 +0.5
2,505,719 + ypdG b2386 0.84 +0.1
2,505,787 + ypdG b2386 0.90 +0.1
2,505,885 - -0.5
2,505,919 + +1.0
2,505,927 - +1.2
2,505,927 - +0.4
2,505,949 + -0.3
2,505,954 - +0.7
2,505,954 - -0.8
2,506,086 - ypdH b2387 0.45 +1.0
2,506,086 - ypdH b2387 0.45 +1.2
2,506,096 + ypdH b2387 0.48 -0.9
2,506,129 - ypdH b2387 0.58 -0.2
2,506,201 - ypdH b2387 0.80 +0.3
2,506,212 - ypdH b2387 0.84 +0.3
2,506,219 - ypdH b2387 0.86 +0.6
2,506,225 - ypdH b2387 0.88 +0.3
2,506,225 - ypdH b2387 0.88 -0.9
2,506,286 + -1.3
2,506,294 - +0.6
2,506,370 + +1.1
2,506,386 - -0.0
2,506,393 + -0.3
2,506,393 + +0.4
2,506,428 + +0.2
2,506,578 - -0.2
2,506,646 - glk b2388 0.17 -0.4
2,506,646 - glk b2388 0.17 -1.1
2,506,731 - glk b2388 0.26 +0.2
2,506,731 - glk b2388 0.26 -0.9
2,506,780 - glk b2388 0.31 -0.8
2,506,800 - glk b2388 0.33 +0.2
2,506,861 - glk b2388 0.39 +0.2
2,506,866 + glk b2388 0.40 +0.2
2,507,004 + glk b2388 0.54 +1.7
2,507,004 + glk b2388 0.54 -1.4
2,507,022 + glk b2388 0.56 +0.3
2,507,057 + glk b2388 0.59 -0.6
2,507,185 + glk b2388 0.73 -1.5
2,507,215 + glk b2388 0.76 -0.2
2,507,333 + glk b2388 0.88 +0.7
2,507,341 - glk b2388 0.89 +0.9
2,507,341 - glk b2388 0.89 -1.4
2,507,362 - -0.9
2,507,362 - -1.1
2,507,362 - -0.6
2,507,392 - -0.7
2,507,407 - +0.5
2,507,448 + +0.1
2,507,483 + -0.4
2,507,493 + +0.1
2,507,499 - -2.5
2,507,506 - -2.8
2,507,513 - -3.1
2,507,540 + -0.1
2,507,634 - -3.0
2,507,634 - -2.5
2,507,634 - -0.5
2,507,803 - yfeO b2389 0.12 -1.9
2,507,805 + yfeO b2389 0.12 -0.0
2,507,835 + yfeO b2389 0.15 +0.1
2,507,883 - yfeO b2389 0.18 -0.5
2,507,883 - yfeO b2389 0.18 -0.7
2,507,901 + yfeO b2389 0.20 -0.0
2,507,904 - yfeO b2389 0.20 -0.7
2,508,013 + yfeO b2389 0.29 +0.4
2,508,302 + yfeO b2389 0.52 +0.6
2,508,328 + yfeO b2389 0.54 -0.2
2,508,368 - yfeO b2389 0.57 +0.4
2,508,377 + yfeO b2389 0.58 -0.3
2,508,377 + yfeO b2389 0.58 +0.3
2,508,377 + yfeO b2389 0.58 +0.1

Or see this region's nucleotide sequence