Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_1583

Experiment: Paraquat dichloride 0.032 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_1582 and PfGW456L13_1583 are separated by 0 nucleotidesPfGW456L13_1583 and PfGW456L13_1584 overlap by 7 nucleotidesPfGW456L13_1584 and PfGW456L13_1585 overlap by 20 nucleotides PfGW456L13_1582 - Peptide chain release factor 1, at 1,747,034 to 1,748,116 _1582 PfGW456L13_1583 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 1,748,117 to 1,748,947 _1583 PfGW456L13_1584 - Molybdopterin biosynthesis protein MoeB, at 1,748,941 to 1,749,696 _1584 PfGW456L13_1585 - Glutamate racemase (EC 5.1.1.3), at 1,749,677 to 1,750,480 _1585 Position (kb) 1748 1749Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1748.106 kb on + strandat 1748.107 kb on - strandat 1748.107 kb on - strandat 1748.107 kb on - strandat 1748.114 kb on + strandat 1748.114 kb on + strandat 1748.114 kb on + strandat 1748.114 kb on + strandat 1748.114 kb on + strandat 1748.114 kb on + strandat 1748.968 kb on + strandat 1749.035 kb on + strand, within PfGW456L13_1584at 1749.079 kb on + strand, within PfGW456L13_1584at 1749.079 kb on + strand, within PfGW456L13_1584at 1749.079 kb on + strand, within PfGW456L13_1584at 1749.454 kb on + strand, within PfGW456L13_1584at 1749.464 kb on + strand, within PfGW456L13_1584

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.032 mg/ml
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1,748,106 + -1.2
1,748,107 - +2.1
1,748,107 - -1.1
1,748,107 - -2.9
1,748,114 + -0.8
1,748,114 + +0.9
1,748,114 + +0.3
1,748,114 + +0.2
1,748,114 + +2.2
1,748,114 + +0.1
1,748,968 + -0.5
1,749,035 + PfGW456L13_1584 0.12 -1.1
1,749,079 + PfGW456L13_1584 0.18 -0.5
1,749,079 + PfGW456L13_1584 0.18 -0.6
1,749,079 + PfGW456L13_1584 0.18 -0.0
1,749,454 + PfGW456L13_1584 0.68 +0.2
1,749,464 + PfGW456L13_1584 0.69 +0.0

Or see this region's nucleotide sequence