Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4254 and EX28DRAFT_4255 are separated by 65 nucleotides EX28DRAFT_4255 and EX28DRAFT_4256 are separated by 0 nucleotides EX28DRAFT_4256 and EX28DRAFT_4257 are separated by 81 nucleotides EX28DRAFT_4257 and EX28DRAFT_4258 are separated by 305 nucleotides
EX28DRAFT_4254: EX28DRAFT_4254 - ribonuclease, at 60,328 to 60,810
_4254
EX28DRAFT_4255: EX28DRAFT_4255 - hypothetical protein, at 60,876 to 61,283
_4255
EX28DRAFT_4256: EX28DRAFT_4256 - hypothetical protein, at 61,284 to 61,574
_4256
EX28DRAFT_4257: EX28DRAFT_4257 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 61,656 to 62,261
_4257
EX28DRAFT_4258: EX28DRAFT_4258 - hypothetical protein, at 62,567 to 63,697
_4258
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 60.733 kb on + strand, within EX28DRAFT_4254 at 60.775 kb on + strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.776 kb on - strand at 60.783 kb on + strand at 60.784 kb on - strand at 60.808 kb on + strand at 60.809 kb on - strand at 60.809 kb on - strand at 60.830 kb on + strand at 60.830 kb on + strand at 60.875 kb on + strand at 60.875 kb on + strand at 60.876 kb on - strand at 60.876 kb on - strand at 60.889 kb on - strand at 60.998 kb on + strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 60.999 kb on - strand, within EX28DRAFT_4255 at 61.010 kb on + strand, within EX28DRAFT_4255 at 61.079 kb on - strand, within EX28DRAFT_4255 at 61.119 kb on - strand, within EX28DRAFT_4255 at 61.136 kb on + strand, within EX28DRAFT_4255 at 61.137 kb on - strand, within EX28DRAFT_4255 at 61.295 kb on - strand at 61.369 kb on - strand, within EX28DRAFT_4256 at 61.662 kb on - strand at 61.669 kb on + strand at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.740 kb on + strand, within EX28DRAFT_4257 at 61.741 kb on - strand, within EX28DRAFT_4257 at 61.846 kb on + strand, within EX28DRAFT_4257 at 61.933 kb on - strand, within EX28DRAFT_4257 at 61.933 kb on - strand, within EX28DRAFT_4257 at 61.968 kb on - strand, within EX28DRAFT_4257 at 62.007 kb on + strand, within EX28DRAFT_4257 at 62.072 kb on + strand, within EX28DRAFT_4257 at 62.114 kb on + strand, within EX28DRAFT_4257 at 62.130 kb on - strand, within EX28DRAFT_4257 at 62.383 kb on + strand at 62.423 kb on + strand at 62.512 kb on - strand at 62.629 kb on + strand at 62.634 kb on - strand at 62.830 kb on + strand, within EX28DRAFT_4258 at 62.834 kb on - strand, within EX28DRAFT_4258 at 62.882 kb on + strand, within EX28DRAFT_4258 at 62.954 kb on + strand, within EX28DRAFT_4258 at 62.955 kb on - strand, within EX28DRAFT_4258 at 62.955 kb on - strand, within EX28DRAFT_4258 at 62.960 kb on + strand, within EX28DRAFT_4258 at 62.960 kb on + strand, within EX28DRAFT_4258 at 62.961 kb on - strand, within EX28DRAFT_4258 at 62.972 kb on - strand, within EX28DRAFT_4258 at 63.076 kb on + strand, within EX28DRAFT_4258 at 63.077 kb on - strand, within EX28DRAFT_4258 at 63.085 kb on + strand, within EX28DRAFT_4258 at 63.085 kb on + strand, within EX28DRAFT_4258 at 63.086 kb on - strand, within EX28DRAFT_4258 at 63.086 kb on - strand, within EX28DRAFT_4258 at 63.086 kb on - strand, within EX28DRAFT_4258 at 63.120 kb on + strand, within EX28DRAFT_4258 at 63.140 kb on - strand, within EX28DRAFT_4258 at 63.218 kb on + strand, within EX28DRAFT_4258 at 63.218 kb on + strand, within EX28DRAFT_4258 at 63.218 kb on + strand, within EX28DRAFT_4258 at 63.218 kb on + strand, within EX28DRAFT_4258 at 63.218 kb on + strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258 at 63.219 kb on - strand, within EX28DRAFT_4258
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 60,733 + EX28DRAFT_4254 0.84 +0.3 60,775 + +0.6 60,776 - -0.5 60,776 - -1.8 60,776 - +0.0 60,776 - +0.1 60,783 + -0.0 60,784 - +0.1 60,808 + -0.3 60,809 - -0.1 60,809 - -0.2 60,830 + -0.0 60,830 + -0.1 60,875 + +0.0 60,875 + +0.3 60,876 - +1.4 60,876 - -0.3 60,889 - -0.3 60,998 + EX28DRAFT_4255 0.30 -0.2 60,999 - EX28DRAFT_4255 0.30 +1.0 60,999 - EX28DRAFT_4255 0.30 +0.1 61,010 + EX28DRAFT_4255 0.33 +0.2 61,079 - EX28DRAFT_4255 0.50 -0.2 61,119 - EX28DRAFT_4255 0.60 -0.6 61,136 + EX28DRAFT_4255 0.64 +0.2 61,137 - EX28DRAFT_4255 0.64 -0.0 61,295 - -0.5 61,369 - EX28DRAFT_4256 0.29 -0.2 61,662 - +0.9 61,669 + +0.7 61,740 + EX28DRAFT_4257 0.14 +0.3 61,740 + EX28DRAFT_4257 0.14 -0.2 61,741 - EX28DRAFT_4257 0.14 +1.0 61,846 + EX28DRAFT_4257 0.31 -0.8 61,933 - EX28DRAFT_4257 0.46 -0.4 61,933 - EX28DRAFT_4257 0.46 -0.0 61,968 - EX28DRAFT_4257 0.51 +1.0 62,007 + EX28DRAFT_4257 0.58 -0.4 62,072 + EX28DRAFT_4257 0.69 -0.5 62,114 + EX28DRAFT_4257 0.76 +1.0 62,130 - EX28DRAFT_4257 0.78 +0.3 62,383 + -2.0 62,423 + -0.4 62,512 - -2.5 62,629 + +1.2 62,634 - -0.9 62,830 + EX28DRAFT_4258 0.23 -0.4 62,834 - EX28DRAFT_4258 0.24 -1.1 62,882 + EX28DRAFT_4258 0.28 -0.8 62,954 + EX28DRAFT_4258 0.34 +0.0 62,955 - EX28DRAFT_4258 0.34 +0.3 62,955 - EX28DRAFT_4258 0.34 -0.1 62,960 + EX28DRAFT_4258 0.35 +0.8 62,960 + EX28DRAFT_4258 0.35 -0.2 62,961 - EX28DRAFT_4258 0.35 +0.2 62,972 - EX28DRAFT_4258 0.36 +0.4 63,076 + EX28DRAFT_4258 0.45 +0.1 63,077 - EX28DRAFT_4258 0.45 +0.5 63,085 + EX28DRAFT_4258 0.46 +0.5 63,085 + EX28DRAFT_4258 0.46 +0.2 63,086 - EX28DRAFT_4258 0.46 -0.9 63,086 - EX28DRAFT_4258 0.46 +0.3 63,086 - EX28DRAFT_4258 0.46 +0.4 63,120 + EX28DRAFT_4258 0.49 +0.2 63,140 - EX28DRAFT_4258 0.51 -2.4 63,218 + EX28DRAFT_4258 0.58 +1.0 63,218 + EX28DRAFT_4258 0.58 -1.0 63,218 + EX28DRAFT_4258 0.58 -0.7 63,218 + EX28DRAFT_4258 0.58 -2.8 63,218 + EX28DRAFT_4258 0.58 -0.1 63,219 - EX28DRAFT_4258 0.58 +1.0 63,219 - EX28DRAFT_4258 0.58 +0.1 63,219 - EX28DRAFT_4258 0.58 -0.1 63,219 - EX28DRAFT_4258 0.58 +0.7 63,219 - EX28DRAFT_4258 0.58 +0.2 63,219 - EX28DRAFT_4258 0.58 +0.3
Or see this region's nucleotide sequence