Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3620 and EX28DRAFT_3621 are separated by 110 nucleotides EX28DRAFT_3621 and EX28DRAFT_3622 are separated by 122 nucleotides EX28DRAFT_3622 and EX28DRAFT_3623 overlap by 24 nucleotides EX28DRAFT_3623 and EX28DRAFT_3624 are separated by 26 nucleotides EX28DRAFT_3624 and EX28DRAFT_3625 are separated by 117 nucleotides
EX28DRAFT_3620: EX28DRAFT_3620 - transaldolase, at 280,176 to 281,129
_3620
EX28DRAFT_3621: EX28DRAFT_3621 - molybdenum cofactor synthesis domain, at 281,240 to 281,827
_3621
EX28DRAFT_3622: EX28DRAFT_3622 - Arabinose efflux permease, at 281,950 to 283,272
_3622
EX28DRAFT_3623: EX28DRAFT_3623 - Predicted membrane protein, at 283,249 to 283,815
_3623
EX28DRAFT_3624: EX28DRAFT_3624 - hypothetical protein, at 283,842 to 283,994
_3624
EX28DRAFT_3625: EX28DRAFT_3625 - chaperone protein DnaK, at 284,112 to 286,025
_3625
Position (kb)
281
282
283
284 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 280.959 kb on - strand, within EX28DRAFT_3620 at 280.969 kb on - strand, within EX28DRAFT_3620 at 281.050 kb on + strand at 281.128 kb on - strand at 281.165 kb on + strand at 281.200 kb on + strand at 281.240 kb on - strand at 281.245 kb on + strand at 281.245 kb on + strand at 281.245 kb on + strand at 281.250 kb on + strand at 281.250 kb on + strand at 281.262 kb on + strand at 281.262 kb on + strand at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.556 kb on + strand, within EX28DRAFT_3621 at 281.563 kb on - strand, within EX28DRAFT_3621 at 281.724 kb on + strand, within EX28DRAFT_3621 at 281.735 kb on - strand, within EX28DRAFT_3621 at 281.756 kb on + strand, within EX28DRAFT_3621 at 281.756 kb on + strand, within EX28DRAFT_3621 at 281.757 kb on - strand, within EX28DRAFT_3621 at 281.818 kb on - strand at 281.838 kb on + strand at 281.838 kb on + strand at 281.838 kb on + strand at 281.839 kb on - strand at 281.849 kb on - strand at 281.881 kb on - strand at 281.950 kb on - strand at 281.958 kb on + strand at 281.958 kb on + strand at 281.959 kb on - strand at 281.959 kb on - strand at 281.986 kb on + strand at 282.069 kb on - strand at 282.205 kb on - strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.274 kb on + strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.275 kb on - strand, within EX28DRAFT_3622 at 282.477 kb on - strand, within EX28DRAFT_3622 at 282.565 kb on + strand, within EX28DRAFT_3622 at 282.659 kb on + strand, within EX28DRAFT_3622 at 282.923 kb on - strand, within EX28DRAFT_3622 at 282.964 kb on + strand, within EX28DRAFT_3622 at 283.040 kb on + strand, within EX28DRAFT_3622 at 283.066 kb on + strand, within EX28DRAFT_3622 at 283.067 kb on - strand, within EX28DRAFT_3622 at 283.079 kb on - strand, within EX28DRAFT_3622 at 283.153 kb on + strand at 283.154 kb on - strand at 283.154 kb on - strand at 283.157 kb on + strand at 283.157 kb on + strand at 283.184 kb on + strand at 283.185 kb on - strand at 283.319 kb on + strand, within EX28DRAFT_3623 at 283.324 kb on - strand, within EX28DRAFT_3623 at 283.642 kb on - strand, within EX28DRAFT_3623 at 283.643 kb on + strand, within EX28DRAFT_3623 at 283.644 kb on - strand, within EX28DRAFT_3623 at 283.644 kb on - strand, within EX28DRAFT_3623 at 283.803 kb on + strand at 283.803 kb on + strand at 283.803 kb on + strand at 283.804 kb on - strand at 283.804 kb on - strand at 283.815 kb on + strand at 283.815 kb on + strand at 283.816 kb on - strand at 283.838 kb on + strand at 283.839 kb on - strand at 283.841 kb on + strand at 283.841 kb on + strand at 283.869 kb on + strand, within EX28DRAFT_3624 at 283.894 kb on + strand, within EX28DRAFT_3624 at 283.917 kb on + strand, within EX28DRAFT_3624 at 283.992 kb on + strand at 284.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 280,959 - EX28DRAFT_3620 0.82 +0.3 280,969 - EX28DRAFT_3620 0.83 +0.5 281,050 + -0.5 281,128 - -0.2 281,165 + +0.3 281,200 + +0.6 281,240 - +0.2 281,245 + -0.2 281,245 + -1.3 281,245 + -0.7 281,250 + +0.3 281,250 + +0.2 281,262 + -0.6 281,262 + -1.0 281,556 + EX28DRAFT_3621 0.54 +2.5 281,556 + EX28DRAFT_3621 0.54 -0.4 281,556 + EX28DRAFT_3621 0.54 -0.3 281,563 - EX28DRAFT_3621 0.55 +0.4 281,724 + EX28DRAFT_3621 0.82 +0.6 281,735 - EX28DRAFT_3621 0.84 -0.7 281,756 + EX28DRAFT_3621 0.88 -1.3 281,756 + EX28DRAFT_3621 0.88 -1.2 281,757 - EX28DRAFT_3621 0.88 +0.4 281,818 - -0.4 281,838 + -0.5 281,838 + +0.2 281,838 + -0.3 281,839 - +0.5 281,849 - -3.7 281,881 - -0.5 281,950 - +0.6 281,958 + +1.1 281,958 + -0.3 281,959 - +0.7 281,959 - -1.2 281,986 + -0.5 282,069 - +1.1 282,205 - EX28DRAFT_3622 0.19 +0.6 282,274 + EX28DRAFT_3622 0.24 -0.2 282,274 + EX28DRAFT_3622 0.24 +0.2 282,274 + EX28DRAFT_3622 0.24 -0.2 282,274 + EX28DRAFT_3622 0.24 -2.0 282,274 + EX28DRAFT_3622 0.24 -0.3 282,274 + EX28DRAFT_3622 0.24 +0.3 282,275 - EX28DRAFT_3622 0.25 +0.2 282,275 - EX28DRAFT_3622 0.25 -0.9 282,275 - EX28DRAFT_3622 0.25 -1.5 282,477 - EX28DRAFT_3622 0.40 +0.5 282,565 + EX28DRAFT_3622 0.46 -0.5 282,659 + EX28DRAFT_3622 0.54 +0.2 282,923 - EX28DRAFT_3622 0.74 +0.0 282,964 + EX28DRAFT_3622 0.77 -0.7 283,040 + EX28DRAFT_3622 0.82 +0.0 283,066 + EX28DRAFT_3622 0.84 -0.3 283,067 - EX28DRAFT_3622 0.84 +1.4 283,079 - EX28DRAFT_3622 0.85 +0.2 283,153 + +0.8 283,154 - +0.2 283,154 - -2.8 283,157 + +0.4 283,157 + -0.4 283,184 + +0.2 283,185 - -0.1 283,319 + EX28DRAFT_3623 0.12 +0.0 283,324 - EX28DRAFT_3623 0.13 +0.1 283,642 - EX28DRAFT_3623 0.69 -0.1 283,643 + EX28DRAFT_3623 0.69 -0.8 283,644 - EX28DRAFT_3623 0.70 -0.2 283,644 - EX28DRAFT_3623 0.70 -0.1 283,803 + +0.1 283,803 + +0.2 283,803 + +0.1 283,804 - -0.7 283,804 - +0.6 283,815 + -0.2 283,815 + -0.6 283,816 - -0.2 283,838 + +0.8 283,839 - +0.4 283,841 + +0.2 283,841 + +0.4 283,869 + EX28DRAFT_3624 0.18 +0.2 283,894 + EX28DRAFT_3624 0.34 -0.1 283,917 + EX28DRAFT_3624 0.49 +0.1 283,992 + -0.1 284,060 + -1.5
Or see this region's nucleotide sequence