Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1916 and EX28DRAFT_1917 are separated by 7 nucleotides EX28DRAFT_1917 and EX28DRAFT_1918 are separated by 9 nucleotides EX28DRAFT_1918 and EX28DRAFT_1919 are separated by 9 nucleotides EX28DRAFT_1919 and EX28DRAFT_1920 are separated by 21 nucleotides
EX28DRAFT_1916: EX28DRAFT_1916 - pyrimidine utilization protein C, at 1,927,257 to 1,927,643
_1916
EX28DRAFT_1917: EX28DRAFT_1917 - pyrimidine utilization protein D, at 1,927,651 to 1,928,451
_1917
EX28DRAFT_1918: EX28DRAFT_1918 - Nitroreductase, at 1,928,461 to 1,929,051
_1918
EX28DRAFT_1919: EX28DRAFT_1919 - pyrimidine utilization flavin reductase protein F, at 1,929,061 to 1,929,555
_1919
EX28DRAFT_1920: EX28DRAFT_1920 - pyrimidine utilization transport protein G, at 1,929,577 to 1,930,899
_1920
Position (kb)
1928
1929
1930 Strain fitness (log2 ratio)
-1
0
1 at 1927.512 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916 at 1927.533 kb on + strand, within EX28DRAFT_1916 at 1927.650 kb on + strand at 1927.651 kb on - strand at 1927.704 kb on + strand at 1927.732 kb on + strand, within EX28DRAFT_1917 at 1927.733 kb on - strand, within EX28DRAFT_1917 at 1927.784 kb on - strand, within EX28DRAFT_1917 at 1928.050 kb on + strand, within EX28DRAFT_1917 at 1928.260 kb on - strand, within EX28DRAFT_1917 at 1928.260 kb on - strand, within EX28DRAFT_1917 at 1928.448 kb on - strand at 1928.449 kb on + strand at 1928.459 kb on + strand at 1928.570 kb on - strand, within EX28DRAFT_1918 at 1928.791 kb on - strand, within EX28DRAFT_1918 at 1928.852 kb on - strand, within EX28DRAFT_1918 at 1929.075 kb on + strand at 1929.212 kb on + strand, within EX28DRAFT_1919 at 1929.212 kb on + strand, within EX28DRAFT_1919 at 1929.302 kb on + strand, within EX28DRAFT_1919 at 1929.303 kb on - strand, within EX28DRAFT_1919 at 1929.553 kb on + strand at 1929.753 kb on + strand, within EX28DRAFT_1920 at 1929.818 kb on - strand, within EX28DRAFT_1920 at 1929.818 kb on - strand, within EX28DRAFT_1920 at 1929.938 kb on - strand, within EX28DRAFT_1920 at 1929.938 kb on - strand, within EX28DRAFT_1920
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,927,512 + EX28DRAFT_1916 0.66 -0.2 1,927,533 + EX28DRAFT_1916 0.71 +0.0 1,927,533 + EX28DRAFT_1916 0.71 +0.2 1,927,650 + +0.0 1,927,651 - +0.2 1,927,704 + -0.8 1,927,732 + EX28DRAFT_1917 0.10 +0.1 1,927,733 - EX28DRAFT_1917 0.10 +0.6 1,927,784 - EX28DRAFT_1917 0.17 -0.1 1,928,050 + EX28DRAFT_1917 0.50 +0.4 1,928,260 - EX28DRAFT_1917 0.76 -0.5 1,928,260 - EX28DRAFT_1917 0.76 -0.4 1,928,448 - +0.0 1,928,449 + -0.6 1,928,459 + -0.2 1,928,570 - EX28DRAFT_1918 0.18 +0.3 1,928,791 - EX28DRAFT_1918 0.56 +0.1 1,928,852 - EX28DRAFT_1918 0.66 +0.3 1,929,075 + -0.0 1,929,212 + EX28DRAFT_1919 0.31 -0.6 1,929,212 + EX28DRAFT_1919 0.31 -1.1 1,929,302 + EX28DRAFT_1919 0.49 -0.3 1,929,303 - EX28DRAFT_1919 0.49 +0.9 1,929,553 + +0.1 1,929,753 + EX28DRAFT_1920 0.13 -1.6 1,929,818 - EX28DRAFT_1920 0.18 +1.1 1,929,818 - EX28DRAFT_1920 0.18 +0.1 1,929,938 - EX28DRAFT_1920 0.27 +0.3 1,929,938 - EX28DRAFT_1920 0.27 -0.1
Or see this region's nucleotide sequence