Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1320 and EX28DRAFT_1321 are separated by 3 nucleotides EX28DRAFT_1321 and EX28DRAFT_1322 are separated by 11 nucleotides EX28DRAFT_1322 and EX28DRAFT_1323 are separated by 11 nucleotides EX28DRAFT_1323 and EX28DRAFT_1324 overlap by 4 nucleotides EX28DRAFT_1324 and EX28DRAFT_1325 overlap by 4 nucleotides
EX28DRAFT_1320: EX28DRAFT_1320 - DNA polymerase III, epsilon subunit and related 3'-5' exonucleases, at 1,320,534 to 1,321,190
_1320
EX28DRAFT_1321: EX28DRAFT_1321 - hypothetical protein, at 1,321,194 to 1,321,859
_1321
EX28DRAFT_1322: EX28DRAFT_1322 - hypothetical protein, at 1,321,871 to 1,322,581
_1322
EX28DRAFT_1323: EX28DRAFT_1323 - Protein of unknown function (DUF1317), at 1,322,593 to 1,322,745
_1323
EX28DRAFT_1324: EX28DRAFT_1324 - Methyltransferase domain, at 1,322,742 to 1,323,224
_1324
EX28DRAFT_1325: EX28DRAFT_1325 - Transcriptional activator, adenine-specific DNA methyltransferase, at 1,323,221 to 1,323,880
_1325
Position (kb)
1321
1322
1323 Strain fitness (log2 ratio)
-1
0
1
2 at 1320.882 kb on + strand, within EX28DRAFT_1320 at 1320.958 kb on - strand, within EX28DRAFT_1320 at 1320.982 kb on - strand, within EX28DRAFT_1320 at 1321.074 kb on - strand, within EX28DRAFT_1320 at 1321.188 kb on + strand at 1321.242 kb on + strand at 1321.243 kb on - strand at 1321.275 kb on - strand, within EX28DRAFT_1321 at 1321.444 kb on + strand, within EX28DRAFT_1321 at 1321.445 kb on - strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.448 kb on + strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.449 kb on - strand, within EX28DRAFT_1321 at 1321.512 kb on + strand, within EX28DRAFT_1321 at 1321.555 kb on + strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.556 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.574 kb on - strand, within EX28DRAFT_1321 at 1321.594 kb on - strand, within EX28DRAFT_1321 at 1321.857 kb on + strand at 1321.857 kb on + strand at 1321.858 kb on - strand at 1321.892 kb on - strand at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.965 kb on - strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.991 kb on + strand, within EX28DRAFT_1322 at 1321.992 kb on - strand, within EX28DRAFT_1322 at 1322.222 kb on - strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.282 kb on + strand, within EX28DRAFT_1322 at 1322.330 kb on + strand, within EX28DRAFT_1322 at 1322.331 kb on - strand, within EX28DRAFT_1322 at 1322.332 kb on + strand, within EX28DRAFT_1322 at 1322.474 kb on - strand, within EX28DRAFT_1322 at 1322.569 kb on + strand at 1322.592 kb on + strand at 1322.593 kb on - strand at 1322.649 kb on + strand, within EX28DRAFT_1323 at 1322.875 kb on + strand, within EX28DRAFT_1324 at 1322.888 kb on + strand, within EX28DRAFT_1324 at 1322.889 kb on - strand, within EX28DRAFT_1324 at 1322.890 kb on + strand, within EX28DRAFT_1324 at 1323.125 kb on + strand, within EX28DRAFT_1324 at 1323.158 kb on - strand, within EX28DRAFT_1324 at 1323.241 kb on + strand at 1323.241 kb on + strand at 1323.245 kb on + strand at 1323.246 kb on - strand at 1323.267 kb on - strand at 1323.386 kb on + strand, within EX28DRAFT_1325
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,320,882 + EX28DRAFT_1320 0.53 -0.2 1,320,958 - EX28DRAFT_1320 0.65 -0.3 1,320,982 - EX28DRAFT_1320 0.68 -0.6 1,321,074 - EX28DRAFT_1320 0.82 -1.1 1,321,188 + -0.2 1,321,242 + +1.2 1,321,243 - +1.1 1,321,275 - EX28DRAFT_1321 0.12 +0.3 1,321,444 + EX28DRAFT_1321 0.38 -0.1 1,321,445 - EX28DRAFT_1321 0.38 -0.2 1,321,448 + EX28DRAFT_1321 0.38 +1.5 1,321,448 + EX28DRAFT_1321 0.38 +0.5 1,321,449 - EX28DRAFT_1321 0.38 -0.3 1,321,449 - EX28DRAFT_1321 0.38 +1.0 1,321,449 - EX28DRAFT_1321 0.38 -0.4 1,321,449 - EX28DRAFT_1321 0.38 -1.2 1,321,512 + EX28DRAFT_1321 0.48 -0.1 1,321,555 + EX28DRAFT_1321 0.54 -0.2 1,321,556 - EX28DRAFT_1321 0.54 +0.4 1,321,556 - EX28DRAFT_1321 0.54 -0.0 1,321,574 - EX28DRAFT_1321 0.57 +0.1 1,321,574 - EX28DRAFT_1321 0.57 -0.3 1,321,574 - EX28DRAFT_1321 0.57 +1.3 1,321,594 - EX28DRAFT_1321 0.60 -0.2 1,321,857 + -0.0 1,321,857 + -0.4 1,321,858 - -0.8 1,321,892 - -0.8 1,321,965 - EX28DRAFT_1322 0.13 +0.7 1,321,965 - EX28DRAFT_1322 0.13 +0.4 1,321,991 + EX28DRAFT_1322 0.17 +0.5 1,321,991 + EX28DRAFT_1322 0.17 -0.0 1,321,992 - EX28DRAFT_1322 0.17 +0.4 1,322,222 - EX28DRAFT_1322 0.49 +0.1 1,322,282 + EX28DRAFT_1322 0.58 -0.4 1,322,282 + EX28DRAFT_1322 0.58 +0.2 1,322,330 + EX28DRAFT_1322 0.65 +0.3 1,322,331 - EX28DRAFT_1322 0.65 +0.5 1,322,332 + EX28DRAFT_1322 0.65 +0.3 1,322,474 - EX28DRAFT_1322 0.85 +1.3 1,322,569 + -0.1 1,322,592 + +1.8 1,322,593 - -0.9 1,322,649 + EX28DRAFT_1323 0.37 +2.1 1,322,875 + EX28DRAFT_1324 0.28 +0.4 1,322,888 + EX28DRAFT_1324 0.30 -0.2 1,322,889 - EX28DRAFT_1324 0.30 +1.0 1,322,890 + EX28DRAFT_1324 0.31 +0.5 1,323,125 + EX28DRAFT_1324 0.79 -0.6 1,323,158 - EX28DRAFT_1324 0.86 -0.2 1,323,241 + -0.1 1,323,241 + -0.0 1,323,245 + +0.4 1,323,246 - +0.1 1,323,267 - +0.4 1,323,386 + EX28DRAFT_1325 0.25 -0.4
Or see this region's nucleotide sequence