Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0173 and EX28DRAFT_0174 overlap by 1 nucleotides EX28DRAFT_0174 and EX28DRAFT_0175 are separated by 3 nucleotides EX28DRAFT_0175 and EX28DRAFT_0176 are separated by 146 nucleotides EX28DRAFT_0176 and EX28DRAFT_0177 overlap by 4 nucleotides
EX28DRAFT_0173: EX28DRAFT_0173 - Leucyl aminopeptidase, at 122,496 to 123,914
_0173
EX28DRAFT_0174: EX28DRAFT_0174 - Asparaginase, at 123,914 to 124,870
_0174
EX28DRAFT_0175: EX28DRAFT_0175 - phosphotransferase system, cellobiose specific, IIC component, at 124,874 to 126,193
_0175
EX28DRAFT_0176: EX28DRAFT_0176 - Homeodomain-like domain, at 126,340 to 127,164
_0176
EX28DRAFT_0177: EX28DRAFT_0177 - arsenate reductase (glutaredoxin), at 127,161 to 127,517
_0177
Position (kb)
124
125
126
127 Strain fitness (log2 ratio)
-2
-1
0
1 at 123.896 kb on + strand at 123.896 kb on + strand at 123.897 kb on - strand at 123.905 kb on - strand at 123.905 kb on - strand at 123.908 kb on + strand at 123.908 kb on + strand at 123.908 kb on + strand at 123.951 kb on + strand at 123.952 kb on - strand at 123.985 kb on - strand at 124.113 kb on - strand, within EX28DRAFT_0174 at 124.202 kb on - strand, within EX28DRAFT_0174 at 124.302 kb on + strand, within EX28DRAFT_0174 at 124.510 kb on + strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.578 kb on + strand, within EX28DRAFT_0174 at 124.660 kb on + strand, within EX28DRAFT_0174 at 124.735 kb on + strand, within EX28DRAFT_0174 at 124.875 kb on + strand at 124.876 kb on - strand at 124.952 kb on - strand at 124.957 kb on + strand at 124.958 kb on - strand at 124.958 kb on - strand at 124.996 kb on + strand at 125.072 kb on - strand, within EX28DRAFT_0175 at 125.119 kb on - strand, within EX28DRAFT_0175 at 125.218 kb on + strand, within EX28DRAFT_0175 at 125.356 kb on + strand, within EX28DRAFT_0175 at 125.515 kb on - strand, within EX28DRAFT_0175 at 125.557 kb on - strand, within EX28DRAFT_0175 at 125.557 kb on - strand, within EX28DRAFT_0175 at 125.580 kb on + strand, within EX28DRAFT_0175 at 125.580 kb on + strand, within EX28DRAFT_0175 at 125.581 kb on - strand, within EX28DRAFT_0175 at 126.016 kb on + strand, within EX28DRAFT_0175 at 126.016 kb on + strand, within EX28DRAFT_0175 at 126.028 kb on - strand, within EX28DRAFT_0175 at 126.099 kb on + strand at 126.232 kb on + strand at 126.499 kb on - strand, within EX28DRAFT_0176 at 126.499 kb on - strand, within EX28DRAFT_0176 at 126.743 kb on + strand, within EX28DRAFT_0176 at 126.743 kb on + strand, within EX28DRAFT_0176 at 126.744 kb on - strand, within EX28DRAFT_0176 at 126.768 kb on + strand, within EX28DRAFT_0176 at 126.769 kb on - strand, within EX28DRAFT_0176 at 126.769 kb on - strand, within EX28DRAFT_0176 at 127.162 kb on + strand at 127.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 123,896 + -0.3 123,896 + +0.4 123,897 - -0.8 123,905 - -0.2 123,905 - +1.4 123,908 + -0.2 123,908 + -1.0 123,908 + +0.2 123,951 + +0.3 123,952 - -0.2 123,985 - +0.1 124,113 - EX28DRAFT_0174 0.21 -0.2 124,202 - EX28DRAFT_0174 0.30 +0.5 124,302 + EX28DRAFT_0174 0.41 +0.5 124,510 + EX28DRAFT_0174 0.62 -0.1 124,511 - EX28DRAFT_0174 0.62 -1.2 124,511 - EX28DRAFT_0174 0.62 -0.1 124,511 - EX28DRAFT_0174 0.62 +0.7 124,511 - EX28DRAFT_0174 0.62 +0.0 124,578 + EX28DRAFT_0174 0.69 -0.5 124,660 + EX28DRAFT_0174 0.78 -0.7 124,735 + EX28DRAFT_0174 0.86 -0.3 124,875 + +0.1 124,876 - +0.6 124,952 - -0.9 124,957 + -0.5 124,958 - +0.1 124,958 - +0.2 124,996 + +0.2 125,072 - EX28DRAFT_0175 0.15 +0.2 125,119 - EX28DRAFT_0175 0.19 +0.5 125,218 + EX28DRAFT_0175 0.26 -0.5 125,356 + EX28DRAFT_0175 0.37 -0.1 125,515 - EX28DRAFT_0175 0.49 -0.1 125,557 - EX28DRAFT_0175 0.52 +0.4 125,557 - EX28DRAFT_0175 0.52 +0.1 125,580 + EX28DRAFT_0175 0.53 +0.3 125,580 + EX28DRAFT_0175 0.53 +0.4 125,581 - EX28DRAFT_0175 0.54 -1.6 126,016 + EX28DRAFT_0175 0.87 +1.2 126,016 + EX28DRAFT_0175 0.87 +0.3 126,028 - EX28DRAFT_0175 0.87 -0.4 126,099 + -1.8 126,232 + -0.3 126,499 - EX28DRAFT_0176 0.19 +1.0 126,499 - EX28DRAFT_0176 0.19 -0.0 126,743 + EX28DRAFT_0176 0.49 -1.1 126,743 + EX28DRAFT_0176 0.49 -0.2 126,744 - EX28DRAFT_0176 0.49 -0.1 126,768 + EX28DRAFT_0176 0.52 +0.7 126,769 - EX28DRAFT_0176 0.52 -0.5 126,769 - EX28DRAFT_0176 0.52 -1.7 127,162 + -0.4 127,162 + -0.0
Or see this region's nucleotide sequence