Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tag and yiaC overlap by 4 nucleotides yiaC and bisC overlap by 32 nucleotides bisC and yiaD are separated by 152 nucleotides yiaD and yiaE are separated by 103 nucleotides
b3549: tag - 3-methyl-adenine DNA glycosylase I, constitutive (NCBI), at 3,711,115 to 3,711,678
tag
b3550: yiaC - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,711,675 to 3,712,115
yiaC
b3551: bisC - biotin sulfoxide reductase (RefSeq), at 3,712,084 to 3,714,417
bisC
b3552: yiaD - predicted outer membrane lipoprotien (NCBI), at 3,714,570 to 3,715,229
yiaD
b3553: yiaE - putative dehydrogenase (VIMSS), at 3,715,333 to 3,716,307
yiaE
Position (kb)
3712
3713
3714
3715 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3711.091 kb on + strand at 3711.123 kb on + strand at 3711.137 kb on + strand at 3711.137 kb on + strand at 3711.155 kb on - strand at 3711.162 kb on + strand at 3711.162 kb on + strand at 3711.183 kb on + strand, within tag at 3711.183 kb on + strand, within tag at 3711.229 kb on + strand, within tag at 3711.298 kb on - strand, within tag at 3711.298 kb on - strand at 3711.352 kb on + strand, within tag at 3711.357 kb on - strand, within tag at 3711.357 kb on - strand, within tag at 3711.360 kb on - strand, within tag at 3711.360 kb on - strand, within tag at 3711.421 kb on - strand, within tag at 3711.433 kb on - strand, within tag at 3711.490 kb on - strand, within tag at 3711.498 kb on + strand, within tag at 3711.500 kb on - strand, within tag at 3711.534 kb on - strand, within tag at 3711.534 kb on - strand, within tag at 3711.613 kb on - strand, within tag at 3711.613 kb on - strand, within tag at 3711.617 kb on + strand, within tag at 3711.617 kb on + strand, within tag at 3711.617 kb on + strand, within tag at 3711.625 kb on - strand at 3711.635 kb on - strand at 3711.645 kb on - strand at 3711.645 kb on - strand at 3711.648 kb on + strand at 3711.702 kb on - strand at 3711.739 kb on - strand, within yiaC at 3711.751 kb on + strand, within yiaC at 3711.751 kb on + strand, within yiaC at 3711.786 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.873 kb on - strand, within yiaC at 3711.874 kb on - strand, within yiaC at 3711.923 kb on - strand, within yiaC at 3711.946 kb on + strand, within yiaC at 3711.946 kb on + strand, within yiaC at 3711.949 kb on + strand, within yiaC at 3711.954 kb on - strand, within yiaC at 3711.954 kb on - strand, within yiaC at 3711.970 kb on + strand, within yiaC at 3711.973 kb on - strand, within yiaC at 3712.058 kb on + strand, within yiaC at 3712.073 kb on + strand at 3712.116 kb on - strand at 3712.118 kb on - strand at 3712.118 kb on - strand at 3712.135 kb on + strand at 3712.135 kb on + strand at 3712.174 kb on + strand at 3712.191 kb on + strand at 3712.191 kb on + strand at 3712.236 kb on - strand at 3712.236 kb on - strand at 3712.305 kb on + strand at 3712.320 kb on + strand, within bisC at 3712.320 kb on + strand, within bisC at 3712.349 kb on + strand, within bisC at 3712.357 kb on - strand, within bisC at 3712.364 kb on + strand, within bisC at 3712.364 kb on + strand, within bisC at 3712.374 kb on - strand, within bisC at 3712.374 kb on - strand, within bisC at 3712.382 kb on + strand, within bisC at 3712.405 kb on - strand at 3712.408 kb on + strand, within bisC at 3712.411 kb on - strand, within bisC at 3712.418 kb on + strand, within bisC at 3712.441 kb on + strand, within bisC at 3712.459 kb on + strand, within bisC at 3712.462 kb on - strand, within bisC at 3712.462 kb on - strand, within bisC at 3712.487 kb on + strand, within bisC at 3712.494 kb on - strand, within bisC at 3712.502 kb on - strand, within bisC at 3712.502 kb on - strand, within bisC at 3712.564 kb on - strand, within bisC at 3712.565 kb on - strand, within bisC at 3712.566 kb on + strand, within bisC at 3712.566 kb on + strand, within bisC at 3712.574 kb on - strand, within bisC at 3712.579 kb on - strand, within bisC at 3712.584 kb on - strand, within bisC at 3712.595 kb on + strand, within bisC at 3712.879 kb on - strand, within bisC at 3712.895 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3712.970 kb on + strand, within bisC at 3713.000 kb on - strand, within bisC at 3713.018 kb on + strand, within bisC at 3713.026 kb on - strand, within bisC at 3713.042 kb on + strand, within bisC at 3713.110 kb on - strand, within bisC at 3713.110 kb on - strand, within bisC at 3713.115 kb on + strand, within bisC at 3713.115 kb on + strand, within bisC at 3713.181 kb on - strand, within bisC at 3713.185 kb on + strand, within bisC at 3713.187 kb on + strand, within bisC at 3713.239 kb on + strand, within bisC at 3713.278 kb on - strand, within bisC at 3713.290 kb on + strand, within bisC at 3713.290 kb on + strand, within bisC at 3713.321 kb on - strand, within bisC at 3713.321 kb on - strand, within bisC at 3713.322 kb on - strand, within bisC at 3713.322 kb on - strand, within bisC at 3713.452 kb on + strand, within bisC at 3713.542 kb on + strand, within bisC at 3713.542 kb on + strand, within bisC at 3713.605 kb on - strand, within bisC at 3713.606 kb on - strand, within bisC at 3713.606 kb on - strand, within bisC at 3713.657 kb on - strand, within bisC at 3713.692 kb on - strand, within bisC at 3713.764 kb on - strand, within bisC at 3713.764 kb on - strand, within bisC at 3713.787 kb on - strand, within bisC at 3713.795 kb on - strand, within bisC at 3713.809 kb on - strand, within bisC at 3713.827 kb on - strand, within bisC at 3713.827 kb on - strand, within bisC at 3713.851 kb on - strand, within bisC at 3713.871 kb on - strand, within bisC at 3713.925 kb on + strand, within bisC at 3713.955 kb on - strand, within bisC at 3713.955 kb on - strand, within bisC at 3713.996 kb on + strand, within bisC at 3714.002 kb on - strand, within bisC at 3714.004 kb on - strand, within bisC at 3714.035 kb on - strand, within bisC at 3714.041 kb on + strand, within bisC at 3714.091 kb on - strand, within bisC at 3714.155 kb on + strand, within bisC at 3714.161 kb on - strand, within bisC at 3714.161 kb on - strand, within bisC at 3714.163 kb on - strand, within bisC at 3714.191 kb on - strand at 3714.191 kb on - strand at 3714.271 kb on + strand at 3714.305 kb on + strand at 3714.313 kb on - strand at 3714.313 kb on - strand at 3714.350 kb on + strand at 3714.426 kb on + strand at 3714.426 kb on + strand at 3714.426 kb on + strand at 3714.434 kb on - strand at 3714.443 kb on + strand at 3714.443 kb on + strand at 3714.492 kb on - strand at 3714.492 kb on - strand at 3714.511 kb on + strand at 3714.511 kb on + strand at 3714.519 kb on - strand at 3714.519 kb on - strand at 3714.564 kb on - strand at 3714.594 kb on - strand at 3714.610 kb on + strand at 3714.610 kb on + strand at 3714.617 kb on + strand at 3714.617 kb on + strand at 3714.621 kb on + strand at 3714.621 kb on + strand at 3714.621 kb on + strand at 3714.629 kb on + strand at 3714.629 kb on + strand at 3714.639 kb on - strand, within yiaD at 3714.641 kb on + strand, within yiaD at 3714.641 kb on + strand, within yiaD at 3714.643 kb on - strand, within yiaD at 3714.643 kb on - strand, within yiaD at 3714.649 kb on - strand, within yiaD at 3714.680 kb on + strand, within yiaD at 3714.687 kb on + strand, within yiaD at 3714.694 kb on + strand, within yiaD at 3714.695 kb on - strand, within yiaD at 3714.710 kb on + strand, within yiaD at 3714.723 kb on + strand, within yiaD at 3714.733 kb on - strand, within yiaD at 3714.733 kb on - strand, within yiaD at 3714.754 kb on - strand, within yiaD at 3714.754 kb on - strand, within yiaD at 3714.766 kb on + strand, within yiaD at 3714.791 kb on - strand, within yiaD at 3714.823 kb on + strand, within yiaD at 3714.856 kb on + strand, within yiaD at 3714.863 kb on - strand, within yiaD at 3714.891 kb on - strand, within yiaD at 3714.905 kb on + strand, within yiaD at 3714.913 kb on - strand, within yiaD at 3714.948 kb on + strand, within yiaD at 3714.957 kb on + strand, within yiaD at 3714.957 kb on + strand, within yiaD at 3714.957 kb on + strand, within yiaD at 3714.967 kb on + strand, within yiaD at 3714.985 kb on - strand, within yiaD at 3714.989 kb on - strand, within yiaD at 3714.989 kb on - strand, within yiaD at 3715.039 kb on - strand, within yiaD at 3715.049 kb on + strand, within yiaD at 3715.061 kb on + strand, within yiaD at 3715.134 kb on - strand, within yiaD at 3715.134 kb on - strand, within yiaD at 3715.174 kb on + strand at 3715.224 kb on + strand at 3715.290 kb on + strand at 3715.290 kb on + strand at 3715.293 kb on - strand at 3715.339 kb on - strand at 3715.381 kb on - strand at 3715.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 3,711,091 + -0.3 3,711,123 + +0.4 3,711,137 + +0.6 3,711,137 + +0.0 3,711,155 - -0.3 3,711,162 + -0.5 3,711,162 + +0.8 3,711,183 + tag b3549 0.12 +0.2 3,711,183 + tag b3549 0.12 -0.4 3,711,229 + tag b3549 0.20 -0.0 3,711,298 - tag b3549 0.32 +0.4 3,711,298 - -0.4 3,711,352 + tag b3549 0.42 -0.4 3,711,357 - tag b3549 0.43 +0.3 3,711,357 - tag b3549 0.43 +0.5 3,711,360 - tag b3549 0.43 -0.1 3,711,360 - tag b3549 0.43 +0.2 3,711,421 - tag b3549 0.54 -0.3 3,711,433 - tag b3549 0.56 -0.6 3,711,490 - tag b3549 0.66 -1.0 3,711,498 + tag b3549 0.68 +0.6 3,711,500 - tag b3549 0.68 -0.0 3,711,534 - tag b3549 0.74 -0.5 3,711,534 - tag b3549 0.74 -0.1 3,711,613 - tag b3549 0.88 +0.6 3,711,613 - tag b3549 0.88 +0.3 3,711,617 + tag b3549 0.89 -0.0 3,711,617 + tag b3549 0.89 -0.1 3,711,617 + tag b3549 0.89 -0.4 3,711,625 - +0.2 3,711,635 - +0.2 3,711,645 - -0.2 3,711,645 - +0.0 3,711,648 + -0.2 3,711,702 - +0.2 3,711,739 - yiaC b3550 0.15 -0.5 3,711,751 + yiaC b3550 0.17 -0.4 3,711,751 + yiaC b3550 0.17 -0.8 3,711,786 - yiaC b3550 0.25 -0.9 3,711,873 - yiaC b3550 0.45 +0.1 3,711,873 - yiaC b3550 0.45 +0.8 3,711,873 - yiaC b3550 0.45 -0.1 3,711,874 - yiaC b3550 0.45 -0.3 3,711,923 - yiaC b3550 0.56 +0.6 3,711,946 + yiaC b3550 0.61 -0.3 3,711,946 + yiaC b3550 0.61 +0.6 3,711,949 + yiaC b3550 0.62 -0.9 3,711,954 - yiaC b3550 0.63 +0.0 3,711,954 - yiaC b3550 0.63 +0.7 3,711,970 + yiaC b3550 0.67 +0.9 3,711,973 - yiaC b3550 0.68 +0.2 3,712,058 + yiaC b3550 0.87 +0.4 3,712,073 + +0.4 3,712,116 - +0.0 3,712,118 - -0.1 3,712,118 - +0.5 3,712,135 + +0.0 3,712,135 + -0.1 3,712,174 + -1.1 3,712,191 + -0.5 3,712,191 + -1.6 3,712,236 - +0.5 3,712,236 - -1.3 3,712,305 + -0.5 3,712,320 + bisC b3551 0.10 +1.1 3,712,320 + bisC b3551 0.10 -1.0 3,712,349 + bisC b3551 0.11 +0.3 3,712,357 - bisC b3551 0.12 -0.4 3,712,364 + bisC b3551 0.12 +0.6 3,712,364 + bisC b3551 0.12 -0.1 3,712,374 - bisC b3551 0.12 -0.3 3,712,374 - bisC b3551 0.12 -0.2 3,712,382 + bisC b3551 0.13 +0.4 3,712,405 - +0.6 3,712,408 + bisC b3551 0.14 +0.3 3,712,411 - bisC b3551 0.14 +0.3 3,712,418 + bisC b3551 0.14 +0.7 3,712,441 + bisC b3551 0.15 -0.6 3,712,459 + bisC b3551 0.16 +0.2 3,712,462 - bisC b3551 0.16 +0.0 3,712,462 - bisC b3551 0.16 +0.9 3,712,487 + bisC b3551 0.17 +0.6 3,712,494 - bisC b3551 0.18 +0.1 3,712,502 - bisC b3551 0.18 +0.4 3,712,502 - bisC b3551 0.18 +0.1 3,712,564 - bisC b3551 0.21 -0.1 3,712,565 - bisC b3551 0.21 +0.3 3,712,566 + bisC b3551 0.21 +0.5 3,712,566 + bisC b3551 0.21 +1.2 3,712,574 - bisC b3551 0.21 -0.0 3,712,579 - bisC b3551 0.21 -0.6 3,712,584 - bisC b3551 0.21 +0.0 3,712,595 + bisC b3551 0.22 +0.6 3,712,879 - bisC b3551 0.34 -0.7 3,712,895 + bisC b3551 0.35 -0.2 3,712,970 + bisC b3551 0.38 +0.6 3,712,970 + bisC b3551 0.38 +0.5 3,712,970 + bisC b3551 0.38 +0.6 3,713,000 - bisC b3551 0.39 +0.6 3,713,018 + bisC b3551 0.40 -0.2 3,713,026 - bisC b3551 0.40 +0.1 3,713,042 + bisC b3551 0.41 +2.6 3,713,110 - bisC b3551 0.44 +0.9 3,713,110 - bisC b3551 0.44 -0.2 3,713,115 + bisC b3551 0.44 +0.2 3,713,115 + bisC b3551 0.44 +0.1 3,713,181 - bisC b3551 0.47 +0.7 3,713,185 + bisC b3551 0.47 +0.2 3,713,187 + bisC b3551 0.47 -0.6 3,713,239 + bisC b3551 0.49 +0.0 3,713,278 - bisC b3551 0.51 +0.5 3,713,290 + bisC b3551 0.52 +0.2 3,713,290 + bisC b3551 0.52 +1.1 3,713,321 - bisC b3551 0.53 -0.0 3,713,321 - bisC b3551 0.53 -1.5 3,713,322 - bisC b3551 0.53 +0.2 3,713,322 - bisC b3551 0.53 -0.6 3,713,452 + bisC b3551 0.59 +0.8 3,713,542 + bisC b3551 0.62 -0.1 3,713,542 + bisC b3551 0.62 -0.2 3,713,605 - bisC b3551 0.65 -0.5 3,713,606 - bisC b3551 0.65 +0.5 3,713,606 - bisC b3551 0.65 +0.2 3,713,657 - bisC b3551 0.67 +0.0 3,713,692 - bisC b3551 0.69 +0.0 3,713,764 - bisC b3551 0.72 +1.1 3,713,764 - bisC b3551 0.72 +0.3 3,713,787 - bisC b3551 0.73 +0.0 3,713,795 - bisC b3551 0.73 +0.0 3,713,809 - bisC b3551 0.74 +0.1 3,713,827 - bisC b3551 0.75 +0.0 3,713,827 - bisC b3551 0.75 -0.8 3,713,851 - bisC b3551 0.76 +0.4 3,713,871 - bisC b3551 0.77 -1.0 3,713,925 + bisC b3551 0.79 -0.3 3,713,955 - bisC b3551 0.80 -0.4 3,713,955 - bisC b3551 0.80 +0.7 3,713,996 + bisC b3551 0.82 +0.4 3,714,002 - bisC b3551 0.82 -0.2 3,714,004 - bisC b3551 0.82 -0.5 3,714,035 - bisC b3551 0.84 -0.2 3,714,041 + bisC b3551 0.84 +0.1 3,714,091 - bisC b3551 0.86 +0.1 3,714,155 + bisC b3551 0.89 +0.9 3,714,161 - bisC b3551 0.89 +0.6 3,714,161 - bisC b3551 0.89 -0.2 3,714,163 - bisC b3551 0.89 -1.1 3,714,191 - +0.0 3,714,191 - +0.8 3,714,271 + -0.3 3,714,305 + +0.4 3,714,313 - +0.3 3,714,313 - +0.4 3,714,350 + -0.9 3,714,426 + -0.3 3,714,426 + -0.7 3,714,426 + -0.1 3,714,434 - +0.3 3,714,443 + +0.3 3,714,443 + +0.1 3,714,492 - +0.4 3,714,492 - +0.7 3,714,511 + -0.8 3,714,511 + +0.0 3,714,519 - +0.2 3,714,519 - +0.5 3,714,564 - -0.0 3,714,594 - +0.2 3,714,610 + -0.3 3,714,610 + +0.1 3,714,617 + +0.2 3,714,617 + +0.2 3,714,621 + +0.1 3,714,621 + +0.3 3,714,621 + -0.0 3,714,629 + +1.1 3,714,629 + +1.1 3,714,639 - yiaD b3552 0.10 +0.2 3,714,641 + yiaD b3552 0.11 +0.1 3,714,641 + yiaD b3552 0.11 -1.6 3,714,643 - yiaD b3552 0.11 +0.7 3,714,643 - yiaD b3552 0.11 +1.4 3,714,649 - yiaD b3552 0.12 -0.2 3,714,680 + yiaD b3552 0.17 +0.6 3,714,687 + yiaD b3552 0.18 -0.1 3,714,694 + yiaD b3552 0.19 +0.3 3,714,695 - yiaD b3552 0.19 +0.4 3,714,710 + yiaD b3552 0.21 -0.1 3,714,723 + yiaD b3552 0.23 -0.1 3,714,733 - yiaD b3552 0.25 -0.2 3,714,733 - yiaD b3552 0.25 -0.6 3,714,754 - yiaD b3552 0.28 +0.6 3,714,754 - yiaD b3552 0.28 -0.1 3,714,766 + yiaD b3552 0.30 +0.9 3,714,791 - yiaD b3552 0.33 -0.1 3,714,823 + yiaD b3552 0.38 -0.5 3,714,856 + yiaD b3552 0.43 -0.7 3,714,863 - yiaD b3552 0.44 +0.1 3,714,891 - yiaD b3552 0.49 -0.2 3,714,905 + yiaD b3552 0.51 +1.2 3,714,913 - yiaD b3552 0.52 -0.5 3,714,948 + yiaD b3552 0.57 -0.4 3,714,957 + yiaD b3552 0.59 +0.8 3,714,957 + yiaD b3552 0.59 -0.5 3,714,957 + yiaD b3552 0.59 +0.0 3,714,967 + yiaD b3552 0.60 -0.2 3,714,985 - yiaD b3552 0.63 -0.5 3,714,989 - yiaD b3552 0.63 -0.1 3,714,989 - yiaD b3552 0.63 +0.4 3,715,039 - yiaD b3552 0.71 +0.5 3,715,049 + yiaD b3552 0.73 +0.4 3,715,061 + yiaD b3552 0.74 +0.0 3,715,134 - yiaD b3552 0.85 +0.0 3,715,134 - yiaD b3552 0.85 +0.4 3,715,174 + +0.1 3,715,224 + +0.7 3,715,290 + -0.1 3,715,290 + +0.8 3,715,293 - -0.2 3,715,339 - -1.2 3,715,381 - +0.3 3,715,395 + -2.1
Or see this region's nucleotide sequence