Experiment: m.b. nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0005 and Echvi_0006 are separated by 15 nucleotides Echvi_0006 and Echvi_0007 overlap by 20 nucleotides Echvi_0007 and Echvi_0008 are separated by 13 nucleotides Echvi_0008 and Echvi_0009 are separated by 291 nucleotides
Echvi_0005: Echvi_0005 - Predicted membrane protein, at 4,135 to 4,671
_0005
Echvi_0006: Echvi_0006 - Uncharacterized protein SCO1/SenC/PrrC, involved in biogenesis of respiratory and photosynthetic systems, at 4,687 to 5,355
_0006
Echvi_0007: Echvi_0007 - Cytochrome c, mono- and diheme variants, at 5,336 to 5,800
_0007
Echvi_0008: Echvi_0008 - hypothetical protein, at 5,814 to 6,212
_0008
Echvi_0009: Echvi_0009 - Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase, at 6,504 to 7,325
_0009
Position (kb) 5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4.677 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.709 kb on + strand at 4.710 kb on - strand at 4.713 kb on + strand at 4.891 kb on - strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.922 kb on + strand, within Echvi_0006 at 4.923 kb on - strand, within Echvi_0006 at 4.924 kb on + strand, within Echvi_0006 at 4.925 kb on - strand, within Echvi_0006 at 4.945 kb on - strand, within Echvi_0006 at 4.996 kb on + strand, within Echvi_0006 at 5.033 kb on + strand, within Echvi_0006 at 5.061 kb on + strand, within Echvi_0006 at 5.076 kb on - strand, within Echvi_0006 at 5.259 kb on + strand at 5.260 kb on - strand, within Echvi_0006 at 5.273 kb on - strand, within Echvi_0006 at 5.434 kb on + strand, within Echvi_0007 at 5.475 kb on - strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.496 kb on + strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.497 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.508 kb on - strand, within Echvi_0007 at 5.661 kb on - strand, within Echvi_0007 at 5.706 kb on + strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.724 kb on - strand, within Echvi_0007 at 5.730 kb on - strand, within Echvi_0007 at 5.799 kb on - strand at 5.802 kb on + strand at 5.818 kb on + strand at 5.818 kb on + strand at 5.894 kb on - strand, within Echvi_0008 at 5.936 kb on + strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.937 kb on - strand, within Echvi_0008 at 5.938 kb on + strand, within Echvi_0008 at 6.014 kb on + strand, within Echvi_0008 at 6.087 kb on + strand, within Echvi_0008 at 6.242 kb on + strand at 6.242 kb on + strand at 6.242 kb on + strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.243 kb on - strand at 6.244 kb on + strand at 6.245 kb on - strand at 6.416 kb on + strand at 6.417 kb on - strand at 6.482 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.488 kb on - strand at 6.496 kb on + strand at 6.497 kb on - strand at 6.514 kb on - strand at 6.533 kb on + strand at 6.533 kb on + strand at 6.635 kb on - strand, within Echvi_0009 at 6.641 kb on - strand, within Echvi_0009 at 6.648 kb on + strand, within Echvi_0009 at 6.649 kb on - strand, within Echvi_0009 at 6.716 kb on + strand, within Echvi_0009 at 6.728 kb on - strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009 at 6.737 kb on + strand, within Echvi_0009
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 20 mM remove 4,677 + +0.7 4,709 + -1.5 4,709 + -0.2 4,709 + -2.2 4,709 + +1.5 4,710 - -2.6 4,713 + -0.6 4,891 - Echvi_0006 0.30 -1.5 4,922 + Echvi_0006 0.35 -2.7 4,922 + Echvi_0006 0.35 -2.1 4,922 + Echvi_0006 0.35 -1.6 4,923 - Echvi_0006 0.35 -0.4 4,924 + Echvi_0006 0.35 -2.7 4,925 - Echvi_0006 0.36 -1.6 4,945 - Echvi_0006 0.39 -3.3 4,996 + Echvi_0006 0.46 -1.2 5,033 + Echvi_0006 0.52 -2.0 5,061 + Echvi_0006 0.56 -3.3 5,076 - Echvi_0006 0.58 -1.5 5,259 + -0.5 5,260 - Echvi_0006 0.86 -1.0 5,273 - Echvi_0006 0.88 -2.1 5,434 + Echvi_0007 0.21 -2.7 5,475 - Echvi_0007 0.30 -2.2 5,496 + Echvi_0007 0.34 -2.4 5,496 + Echvi_0007 0.34 -0.8 5,496 + Echvi_0007 0.34 -2.5 5,496 + Echvi_0007 0.34 -1.6 5,497 - Echvi_0007 0.35 -3.1 5,497 - Echvi_0007 0.35 -1.5 5,508 - Echvi_0007 0.37 -0.9 5,508 - Echvi_0007 0.37 -0.2 5,661 - Echvi_0007 0.70 -2.4 5,706 + Echvi_0007 0.80 -2.1 5,724 - Echvi_0007 0.83 -3.1 5,724 - Echvi_0007 0.83 -0.5 5,730 - Echvi_0007 0.85 +0.1 5,799 - +0.0 5,802 + -0.3 5,818 + -0.6 5,818 + +0.4 5,894 - Echvi_0008 0.20 -1.1 5,936 + Echvi_0008 0.31 -2.9 5,937 - Echvi_0008 0.31 -1.3 5,937 - Echvi_0008 0.31 -1.1 5,938 + Echvi_0008 0.31 -1.4 6,014 + Echvi_0008 0.50 -3.1 6,087 + Echvi_0008 0.68 -3.1 6,242 + -1.2 6,242 + +0.4 6,242 + -0.3 6,243 - -0.8 6,243 - -0.7 6,243 - +0.8 6,243 - +0.1 6,244 + -1.5 6,245 - -0.3 6,416 + +0.1 6,417 - -0.5 6,482 - -1.4 6,488 - -1.1 6,488 - -0.4 6,488 - -1.2 6,496 + -0.5 6,497 - -1.4 6,514 - -0.8 6,533 + -0.1 6,533 + -1.2 6,635 - Echvi_0009 0.16 -1.5 6,641 - Echvi_0009 0.17 +0.2 6,648 + Echvi_0009 0.18 -3.0 6,649 - Echvi_0009 0.18 -0.5 6,716 + Echvi_0009 0.26 +0.1 6,728 - Echvi_0009 0.27 -1.2 6,737 + Echvi_0009 0.28 -0.8 6,737 + Echvi_0009 0.28 -0.2 6,737 + Echvi_0009 0.28 -0.0
Or see this region's nucleotide sequence