Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4531 and EX28DRAFT_4532 overlap by 4 nucleotides EX28DRAFT_4532 and EX28DRAFT_4533 are separated by 1 nucleotides EX28DRAFT_4533 and EX28DRAFT_4534 overlap by 17 nucleotides EX28DRAFT_4534 and EX28DRAFT_4535 overlap by 14 nucleotides
EX28DRAFT_4531: EX28DRAFT_4531 - thiazole biosynthesis protein ThiH, at 15,261 to 16,388
_4531
EX28DRAFT_4532: EX28DRAFT_4532 - Uncharacterized enzyme of thiazole biosynthesis, at 16,385 to 17,155
_4532
EX28DRAFT_4533: EX28DRAFT_4533 - thiamine biosynthesis protein ThiS, at 17,157 to 17,357
_4533
EX28DRAFT_4534: EX28DRAFT_4534 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 17,341 to 18,096
_4534
EX28DRAFT_4535: EX28DRAFT_4535 - thiamine-phosphate diphosphorylase, at 18,083 to 18,724
_4535
Position (kb) 16
17
18 Strain fitness (log2 ratio) -1
0
1 at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.678 kb on - strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.679 kb on + strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.680 kb on - strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.889 kb on + strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.890 kb on - strand, within EX28DRAFT_4531 at 15.970 kb on + strand, within EX28DRAFT_4531 at 16.083 kb on - strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.117 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.150 kb on + strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 16.163 kb on - strand, within EX28DRAFT_4531 at 17.015 kb on + strand, within EX28DRAFT_4532 at 17.016 kb on - strand, within EX28DRAFT_4532 at 17.147 kb on - strand at 17.147 kb on - strand at 17.151 kb on - strand at 17.509 kb on + strand, within EX28DRAFT_4534 at 17.671 kb on - strand, within EX28DRAFT_4534
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 15,678 - EX28DRAFT_4531 0.37 +1.0 15,678 - EX28DRAFT_4531 0.37 +0.1 15,679 + EX28DRAFT_4531 0.37 -0.1 15,679 + EX28DRAFT_4531 0.37 +0.1 15,680 - EX28DRAFT_4531 0.37 +1.2 15,680 - EX28DRAFT_4531 0.37 +0.6 15,889 + EX28DRAFT_4531 0.56 +0.9 15,889 + EX28DRAFT_4531 0.56 -0.2 15,890 - EX28DRAFT_4531 0.56 +0.4 15,890 - EX28DRAFT_4531 0.56 -0.4 15,970 + EX28DRAFT_4531 0.63 -0.0 16,083 - EX28DRAFT_4531 0.73 +0.3 16,117 + EX28DRAFT_4531 0.76 +0.9 16,117 + EX28DRAFT_4531 0.76 -0.6 16,150 + EX28DRAFT_4531 0.79 +0.3 16,150 + EX28DRAFT_4531 0.79 -0.8 16,163 - EX28DRAFT_4531 0.80 +0.2 16,163 - EX28DRAFT_4531 0.80 -0.8 16,163 - EX28DRAFT_4531 0.80 +1.0 17,015 + EX28DRAFT_4532 0.82 +0.1 17,016 - EX28DRAFT_4532 0.82 +0.1 17,147 - -0.3 17,147 - -0.1 17,151 - -0.6 17,509 + EX28DRAFT_4534 0.22 +0.6 17,671 - EX28DRAFT_4534 0.44 +0.1
Or see this region's nucleotide sequence