Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4502 and EX28DRAFT_4503 are separated by 2 nucleotides EX28DRAFT_4503 and EX28DRAFT_4504 are separated by 41 nucleotides EX28DRAFT_4504 and EX28DRAFT_4505 overlap by 4 nucleotides EX28DRAFT_4505 and EX28DRAFT_4506 are separated by 170 nucleotides
EX28DRAFT_4502: EX28DRAFT_4502 - twin arginine-targeting protein translocase TatB, at 68,954 to 69,496
_4502
EX28DRAFT_4503: EX28DRAFT_4503 - Twin arginine targeting (Tat) protein translocase TatC, at 69,499 to 70,269
_4503
EX28DRAFT_4504: EX28DRAFT_4504 - Mg-dependent DNase, at 70,311 to 71,093
_4504
EX28DRAFT_4505: EX28DRAFT_4505 - transcription elongation factor/antiterminator RfaH, at 71,090 to 71,581
_4505
EX28DRAFT_4506: EX28DRAFT_4506 - UbiD family decarboxylase, at 71,752 to 73,245
_4506
Position (kb) 70
71
72 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 69.494 kb on + strand at 69.506 kb on + strand at 69.506 kb on + strand at 69.506 kb on + strand at 69.507 kb on - strand at 69.507 kb on - strand at 69.507 kb on - strand at 69.622 kb on + strand, within EX28DRAFT_4503 at 69.623 kb on - strand, within EX28DRAFT_4503 at 69.623 kb on - strand, within EX28DRAFT_4503 at 69.623 kb on - strand, within EX28DRAFT_4503 at 69.798 kb on + strand, within EX28DRAFT_4503 at 69.799 kb on - strand, within EX28DRAFT_4503 at 69.853 kb on + strand, within EX28DRAFT_4503 at 70.068 kb on + strand, within EX28DRAFT_4503 at 70.081 kb on + strand, within EX28DRAFT_4503 at 70.082 kb on - strand, within EX28DRAFT_4503 at 70.082 kb on - strand, within EX28DRAFT_4503 at 70.083 kb on + strand, within EX28DRAFT_4503 at 70.084 kb on - strand, within EX28DRAFT_4503 at 70.166 kb on - strand, within EX28DRAFT_4503 at 70.188 kb on - strand, within EX28DRAFT_4503 at 70.205 kb on - strand at 70.242 kb on + strand at 70.242 kb on + strand at 70.296 kb on + strand at 70.296 kb on + strand at 70.296 kb on + strand at 70.297 kb on - strand at 70.297 kb on - strand at 70.297 kb on - strand at 70.297 kb on - strand at 70.605 kb on - strand, within EX28DRAFT_4504 at 70.685 kb on + strand, within EX28DRAFT_4504 at 70.818 kb on + strand, within EX28DRAFT_4504 at 70.927 kb on - strand, within EX28DRAFT_4504 at 70.944 kb on - strand, within EX28DRAFT_4504 at 70.983 kb on + strand, within EX28DRAFT_4504 at 71.092 kb on - strand at 71.116 kb on + strand at 71.130 kb on - strand at 71.161 kb on + strand, within EX28DRAFT_4505 at 71.162 kb on - strand, within EX28DRAFT_4505 at 71.162 kb on - strand, within EX28DRAFT_4505 at 71.225 kb on - strand, within EX28DRAFT_4505 at 71.286 kb on + strand, within EX28DRAFT_4505 at 71.286 kb on + strand, within EX28DRAFT_4505 at 71.422 kb on - strand, within EX28DRAFT_4505 at 71.565 kb on - strand at 71.569 kb on - strand at 71.581 kb on + strand at 71.581 kb on + strand at 71.582 kb on - strand at 71.582 kb on - strand at 71.647 kb on + strand at 71.648 kb on - strand at 71.650 kb on - strand at 71.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 69,494 + -0.3 69,506 + -2.2 69,506 + -0.4 69,506 + -0.2 69,507 - -1.5 69,507 - -1.4 69,507 - -1.2 69,622 + EX28DRAFT_4503 0.16 +0.2 69,623 - EX28DRAFT_4503 0.16 -1.0 69,623 - EX28DRAFT_4503 0.16 -0.8 69,623 - EX28DRAFT_4503 0.16 -1.4 69,798 + EX28DRAFT_4503 0.39 -0.3 69,799 - EX28DRAFT_4503 0.39 -1.8 69,853 + EX28DRAFT_4503 0.46 -0.6 70,068 + EX28DRAFT_4503 0.74 +0.4 70,081 + EX28DRAFT_4503 0.75 -0.2 70,082 - EX28DRAFT_4503 0.76 -1.3 70,082 - EX28DRAFT_4503 0.76 +0.4 70,083 + EX28DRAFT_4503 0.76 -0.7 70,084 - EX28DRAFT_4503 0.76 -0.9 70,166 - EX28DRAFT_4503 0.87 -1.0 70,188 - EX28DRAFT_4503 0.89 -0.7 70,205 - -0.3 70,242 + -0.1 70,242 + +0.8 70,296 + -0.0 70,296 + -0.2 70,296 + +0.3 70,297 - -0.1 70,297 - +0.5 70,297 - +0.2 70,297 - +0.1 70,605 - EX28DRAFT_4504 0.38 -0.1 70,685 + EX28DRAFT_4504 0.48 -0.5 70,818 + EX28DRAFT_4504 0.65 +0.3 70,927 - EX28DRAFT_4504 0.79 +0.2 70,944 - EX28DRAFT_4504 0.81 +0.5 70,983 + EX28DRAFT_4504 0.86 +0.1 71,092 - -0.4 71,116 + +0.7 71,130 - +1.3 71,161 + EX28DRAFT_4505 0.14 +0.2 71,162 - EX28DRAFT_4505 0.15 -0.1 71,162 - EX28DRAFT_4505 0.15 -4.3 71,225 - EX28DRAFT_4505 0.27 +0.4 71,286 + EX28DRAFT_4505 0.40 +0.2 71,286 + EX28DRAFT_4505 0.40 +0.9 71,422 - EX28DRAFT_4505 0.67 +0.8 71,565 - +0.6 71,569 - +1.6 71,581 + -0.1 71,581 + +0.4 71,582 - -0.1 71,582 - +0.9 71,647 + -1.2 71,648 - +0.7 71,650 - -0.7 71,727 - +1.1
Or see this region's nucleotide sequence