Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4371 and EX28DRAFT_4372 are separated by 199 nucleotides EX28DRAFT_4372 and EX28DRAFT_4373 are separated by 2 nucleotides EX28DRAFT_4373 and EX28DRAFT_4374 are separated by 39 nucleotides EX28DRAFT_4374 and EX28DRAFT_4375 overlap by 4 nucleotides EX28DRAFT_4375 and EX28DRAFT_4376 overlap by 4 nucleotides
EX28DRAFT_4371: EX28DRAFT_4371 - Esterase/lipase, at 36,912 to 37,832
_4371
EX28DRAFT_4372: EX28DRAFT_4372 - Ribbon-helix-helix protein, copG family, at 38,032 to 38,274
_4372
EX28DRAFT_4373: EX28DRAFT_4373 - Predicted nucleic acid-binding protein, contains PIN domain, at 38,277 to 38,648
_4373
EX28DRAFT_4374: EX28DRAFT_4374 - formate dehydrogenase accessory protein FdhE, at 38,688 to 39,617
_4374
EX28DRAFT_4375: EX28DRAFT_4375 - formate dehydrogenase, gamma subunit, at 39,614 to 40,249
_4375
EX28DRAFT_4376: EX28DRAFT_4376 - formate dehydrogenase, beta subunit, Fe-S containing, at 40,246 to 41,148
_4376
Position (kb)
38
39
40 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 37.732 kb on + strand, within EX28DRAFT_4371 at 37.732 kb on + strand, within EX28DRAFT_4371 at 37.732 kb on + strand, within EX28DRAFT_4371 at 37.733 kb on - strand, within EX28DRAFT_4371 at 37.859 kb on + strand at 37.860 kb on - strand at 37.879 kb on + strand at 37.880 kb on - strand at 37.953 kb on + strand at 37.953 kb on + strand at 37.987 kb on + strand at 37.987 kb on + strand at 37.988 kb on - strand at 38.019 kb on - strand at 38.019 kb on - strand at 38.022 kb on - strand at 38.031 kb on + strand at 38.031 kb on + strand at 38.032 kb on - strand at 38.032 kb on - strand at 38.090 kb on + strand, within EX28DRAFT_4372 at 38.091 kb on - strand, within EX28DRAFT_4372 at 38.167 kb on + strand, within EX28DRAFT_4372 at 38.168 kb on - strand, within EX28DRAFT_4372 at 38.246 kb on - strand, within EX28DRAFT_4372 at 38.272 kb on + strand at 38.273 kb on - strand at 38.307 kb on - strand at 38.307 kb on - strand at 38.307 kb on - strand at 38.307 kb on - strand at 38.325 kb on - strand, within EX28DRAFT_4373 at 38.597 kb on + strand, within EX28DRAFT_4373 at 38.646 kb on + strand at 38.685 kb on + strand at 38.685 kb on + strand at 38.685 kb on + strand at 38.689 kb on + strand at 38.689 kb on + strand at 38.689 kb on + strand at 38.837 kb on - strand, within EX28DRAFT_4374 at 38.837 kb on - strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.900 kb on - strand, within EX28DRAFT_4374 at 38.900 kb on - strand, within EX28DRAFT_4374 at 39.119 kb on - strand, within EX28DRAFT_4374 at 39.141 kb on - strand, within EX28DRAFT_4374 at 39.556 kb on + strand at 39.557 kb on - strand at 39.655 kb on - strand at 39.686 kb on - strand, within EX28DRAFT_4375 at 39.744 kb on + strand, within EX28DRAFT_4375 at 39.744 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.844 kb on - strand, within EX28DRAFT_4375 at 39.892 kb on + strand, within EX28DRAFT_4375 at 39.931 kb on - strand, within EX28DRAFT_4375 at 39.971 kb on - strand, within EX28DRAFT_4375 at 39.971 kb on - strand, within EX28DRAFT_4375 at 39.977 kb on - strand, within EX28DRAFT_4375 at 39.992 kb on + strand, within EX28DRAFT_4375 at 39.992 kb on + strand, within EX28DRAFT_4375 at 39.993 kb on - strand, within EX28DRAFT_4375 at 39.994 kb on + strand, within EX28DRAFT_4375 at 40.010 kb on + strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.015 kb on + strand, within EX28DRAFT_4375 at 40.045 kb on + strand, within EX28DRAFT_4375 at 40.221 kb on + strand at 40.323 kb on - strand at 40.472 kb on + strand, within EX28DRAFT_4376 at 40.473 kb on - strand, within EX28DRAFT_4376 at 40.473 kb on - strand, within EX28DRAFT_4376 at 40.575 kb on + strand, within EX28DRAFT_4376 at 40.576 kb on - strand, within EX28DRAFT_4376
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 37,732 + EX28DRAFT_4371 0.89 -3.3 37,732 + EX28DRAFT_4371 0.89 -0.5 37,732 + EX28DRAFT_4371 0.89 +1.6 37,733 - EX28DRAFT_4371 0.89 -0.4 37,859 + -1.0 37,860 - +1.0 37,879 + -1.5 37,880 - +0.1 37,953 + +0.6 37,953 + +1.0 37,987 + -0.4 37,987 + +0.2 37,988 - -0.2 38,019 - +1.0 38,019 - -0.5 38,022 - -2.1 38,031 + +0.3 38,031 + +1.1 38,032 - -3.6 38,032 - -0.1 38,090 + EX28DRAFT_4372 0.24 +0.7 38,091 - EX28DRAFT_4372 0.24 -0.6 38,167 + EX28DRAFT_4372 0.56 +0.2 38,168 - EX28DRAFT_4372 0.56 +0.2 38,246 - EX28DRAFT_4372 0.88 -0.1 38,272 + +0.2 38,273 - +0.3 38,307 - +0.9 38,307 - +0.6 38,307 - -0.8 38,307 - -0.4 38,325 - EX28DRAFT_4373 0.13 -0.1 38,597 + EX28DRAFT_4373 0.86 +0.3 38,646 + -0.1 38,685 + -0.4 38,685 + +2.7 38,685 + -1.8 38,689 + +0.4 38,689 + -0.4 38,689 + -0.3 38,837 - EX28DRAFT_4374 0.16 -0.6 38,837 - EX28DRAFT_4374 0.16 -1.1 38,899 + EX28DRAFT_4374 0.23 +0.2 38,899 + EX28DRAFT_4374 0.23 +0.9 38,899 + EX28DRAFT_4374 0.23 +0.1 38,900 - EX28DRAFT_4374 0.23 -2.6 38,900 - EX28DRAFT_4374 0.23 +0.8 39,119 - EX28DRAFT_4374 0.46 +0.3 39,141 - EX28DRAFT_4374 0.49 +2.6 39,556 + -0.1 39,557 - +0.3 39,655 - -0.3 39,686 - EX28DRAFT_4375 0.11 -0.7 39,744 + EX28DRAFT_4375 0.20 +0.6 39,744 + EX28DRAFT_4375 0.20 +0.6 39,748 + EX28DRAFT_4375 0.21 -0.2 39,748 + EX28DRAFT_4375 0.21 -0.3 39,748 + EX28DRAFT_4375 0.21 +0.2 39,749 - EX28DRAFT_4375 0.21 -0.4 39,749 - EX28DRAFT_4375 0.21 +0.1 39,749 - EX28DRAFT_4375 0.21 +0.5 39,749 - EX28DRAFT_4375 0.21 +0.0 39,749 - EX28DRAFT_4375 0.21 -0.2 39,844 - EX28DRAFT_4375 0.36 -0.1 39,892 + EX28DRAFT_4375 0.44 +0.6 39,931 - EX28DRAFT_4375 0.50 -0.7 39,971 - EX28DRAFT_4375 0.56 +0.2 39,971 - EX28DRAFT_4375 0.56 +0.0 39,977 - EX28DRAFT_4375 0.57 +0.1 39,992 + EX28DRAFT_4375 0.59 +0.5 39,992 + EX28DRAFT_4375 0.59 +0.1 39,993 - EX28DRAFT_4375 0.60 +0.3 39,994 + EX28DRAFT_4375 0.60 +0.3 40,010 + EX28DRAFT_4375 0.62 -0.7 40,011 - EX28DRAFT_4375 0.62 +0.4 40,011 - EX28DRAFT_4375 0.62 -2.2 40,011 - EX28DRAFT_4375 0.62 +1.0 40,011 - EX28DRAFT_4375 0.62 -0.3 40,015 + EX28DRAFT_4375 0.63 +0.2 40,045 + EX28DRAFT_4375 0.68 +0.4 40,221 + +0.5 40,323 - +0.0 40,472 + EX28DRAFT_4376 0.25 -0.2 40,473 - EX28DRAFT_4376 0.25 +0.2 40,473 - EX28DRAFT_4376 0.25 -0.4 40,575 + EX28DRAFT_4376 0.36 -0.8 40,576 - EX28DRAFT_4376 0.37 -0.4
Or see this region's nucleotide sequence