Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_4162

Experiment: MS_media with arsenate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_4160 and EX28DRAFT_4161 are separated by 135 nucleotidesEX28DRAFT_4161 and EX28DRAFT_4162 overlap by 4 nucleotidesEX28DRAFT_4162 and EX28DRAFT_4163 are separated by 239 nucleotides EX28DRAFT_4160: EX28DRAFT_4160 - uroporphyrin-III C-methyltransferase, at 140,215 to 141,588 _4160 EX28DRAFT_4161: EX28DRAFT_4161 - nitrite reductase [NAD(P)H], small subunit, at 141,724 to 142,050 _4161 EX28DRAFT_4162: EX28DRAFT_4162 - nitrite reductase [NAD(P)H], large subunit, at 142,047 to 144,590 _4162 EX28DRAFT_4163: EX28DRAFT_4163 - Cytosine deaminase and related metal-dependent hydrolases, at 144,830 to 146,146 _4163 Position (kb) 142 143 144 145Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 141.414 kb on + strand, within EX28DRAFT_4160at 141.595 kb on - strandat 141.695 kb on + strandat 141.696 kb on - strandat 141.696 kb on - strandat 141.725 kb on + strandat 141.767 kb on + strand, within EX28DRAFT_4161at 141.767 kb on + strand, within EX28DRAFT_4161at 141.768 kb on - strand, within EX28DRAFT_4161at 141.919 kb on - strand, within EX28DRAFT_4161at 142.033 kb on - strandat 142.079 kb on + strandat 142.079 kb on + strandat 142.079 kb on + strandat 142.088 kb on - strandat 142.131 kb on + strandat 142.131 kb on + strandat 142.132 kb on - strandat 142.132 kb on - strandat 142.304 kb on - strand, within EX28DRAFT_4162at 142.372 kb on + strand, within EX28DRAFT_4162at 142.372 kb on + strand, within EX28DRAFT_4162at 142.478 kb on - strand, within EX28DRAFT_4162at 142.810 kb on + strand, within EX28DRAFT_4162at 142.811 kb on - strand, within EX28DRAFT_4162at 142.833 kb on - strand, within EX28DRAFT_4162at 142.898 kb on - strand, within EX28DRAFT_4162at 142.955 kb on + strand, within EX28DRAFT_4162at 142.975 kb on + strand, within EX28DRAFT_4162at 142.975 kb on + strand, within EX28DRAFT_4162at 143.075 kb on + strand, within EX28DRAFT_4162at 143.075 kb on + strand, within EX28DRAFT_4162at 143.076 kb on - strand, within EX28DRAFT_4162at 143.119 kb on + strand, within EX28DRAFT_4162at 143.119 kb on + strand, within EX28DRAFT_4162at 143.119 kb on + strand, within EX28DRAFT_4162at 143.496 kb on - strand, within EX28DRAFT_4162at 143.524 kb on + strand, within EX28DRAFT_4162at 143.524 kb on + strand, within EX28DRAFT_4162at 143.525 kb on - strand, within EX28DRAFT_4162at 143.525 kb on - strand, within EX28DRAFT_4162at 143.548 kb on + strand, within EX28DRAFT_4162at 143.548 kb on + strand, within EX28DRAFT_4162at 143.549 kb on - strand, within EX28DRAFT_4162at 143.554 kb on + strand, within EX28DRAFT_4162at 143.554 kb on + strand, within EX28DRAFT_4162at 143.554 kb on + strand, within EX28DRAFT_4162at 143.554 kb on + strand, within EX28DRAFT_4162at 143.555 kb on - strand, within EX28DRAFT_4162at 143.555 kb on - strand, within EX28DRAFT_4162at 143.555 kb on - strand, within EX28DRAFT_4162at 143.601 kb on + strand, within EX28DRAFT_4162at 143.672 kb on + strand, within EX28DRAFT_4162at 143.724 kb on + strand, within EX28DRAFT_4162at 143.725 kb on - strand, within EX28DRAFT_4162at 143.761 kb on + strand, within EX28DRAFT_4162at 143.761 kb on + strand, within EX28DRAFT_4162at 143.762 kb on - strand, within EX28DRAFT_4162at 143.864 kb on + strand, within EX28DRAFT_4162at 144.436 kb on + strandat 144.436 kb on + strandat 144.436 kb on + strandat 144.437 kb on - strandat 144.536 kb on + strandat 144.647 kb on + strandat 144.829 kb on + strandat 144.835 kb on + strandat 144.835 kb on + strandat 144.835 kb on + strandat 144.835 kb on + strandat 144.836 kb on - strandat 144.966 kb on + strand, within EX28DRAFT_4163at 144.966 kb on + strand, within EX28DRAFT_4163at 144.967 kb on - strand, within EX28DRAFT_4163at 145.451 kb on + strand, within EX28DRAFT_4163at 145.452 kb on - strand, within EX28DRAFT_4163at 145.589 kb on + strand, within EX28DRAFT_4163at 145.590 kb on - strand, within EX28DRAFT_4163

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Per-strain Table

Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM
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141,414 + EX28DRAFT_4160 0.87 -0.5
141,595 - -2.2
141,695 + -0.3
141,696 - +0.7
141,696 - +0.3
141,725 + -0.2
141,767 + EX28DRAFT_4161 0.13 +0.5
141,767 + EX28DRAFT_4161 0.13 +0.3
141,768 - EX28DRAFT_4161 0.13 +0.3
141,919 - EX28DRAFT_4161 0.60 -2.8
142,033 - -0.3
142,079 + -0.4
142,079 + +0.6
142,079 + -0.5
142,088 - +0.3
142,131 + -0.8
142,131 + +0.7
142,132 - +0.6
142,132 - +0.6
142,304 - EX28DRAFT_4162 0.10 +0.0
142,372 + EX28DRAFT_4162 0.13 +2.8
142,372 + EX28DRAFT_4162 0.13 +0.2
142,478 - EX28DRAFT_4162 0.17 -0.0
142,810 + EX28DRAFT_4162 0.30 -0.0
142,811 - EX28DRAFT_4162 0.30 -0.0
142,833 - EX28DRAFT_4162 0.31 -0.4
142,898 - EX28DRAFT_4162 0.33 -0.6
142,955 + EX28DRAFT_4162 0.36 +1.3
142,975 + EX28DRAFT_4162 0.36 +0.5
142,975 + EX28DRAFT_4162 0.36 +0.0
143,075 + EX28DRAFT_4162 0.40 +0.2
143,075 + EX28DRAFT_4162 0.40 -0.6
143,076 - EX28DRAFT_4162 0.40 +1.8
143,119 + EX28DRAFT_4162 0.42 +0.6
143,119 + EX28DRAFT_4162 0.42 +0.4
143,119 + EX28DRAFT_4162 0.42 +0.6
143,496 - EX28DRAFT_4162 0.57 -2.7
143,524 + EX28DRAFT_4162 0.58 -1.4
143,524 + EX28DRAFT_4162 0.58 -1.0
143,525 - EX28DRAFT_4162 0.58 -1.4
143,525 - EX28DRAFT_4162 0.58 -0.6
143,548 + EX28DRAFT_4162 0.59 +0.2
143,548 + EX28DRAFT_4162 0.59 +0.7
143,549 - EX28DRAFT_4162 0.59 +1.1
143,554 + EX28DRAFT_4162 0.59 +0.1
143,554 + EX28DRAFT_4162 0.59 +0.5
143,554 + EX28DRAFT_4162 0.59 +0.1
143,554 + EX28DRAFT_4162 0.59 +0.6
143,555 - EX28DRAFT_4162 0.59 -0.3
143,555 - EX28DRAFT_4162 0.59 -4.0
143,555 - EX28DRAFT_4162 0.59 +0.2
143,601 + EX28DRAFT_4162 0.61 -0.3
143,672 + EX28DRAFT_4162 0.64 +0.9
143,724 + EX28DRAFT_4162 0.66 +1.4
143,725 - EX28DRAFT_4162 0.66 -0.8
143,761 + EX28DRAFT_4162 0.67 +0.1
143,761 + EX28DRAFT_4162 0.67 -0.1
143,762 - EX28DRAFT_4162 0.67 -0.4
143,864 + EX28DRAFT_4162 0.71 +2.1
144,436 + +0.3
144,436 + +1.9
144,436 + -0.3
144,437 - +0.3
144,536 + -0.5
144,647 + +0.6
144,829 + +0.7
144,835 + -0.1
144,835 + -3.5
144,835 + -0.1
144,835 + -0.2
144,836 - +2.3
144,966 + EX28DRAFT_4163 0.10 +0.4
144,966 + EX28DRAFT_4163 0.10 -0.2
144,967 - EX28DRAFT_4163 0.10 -0.3
145,451 + EX28DRAFT_4163 0.47 -0.1
145,452 - EX28DRAFT_4163 0.47 -0.1
145,589 + EX28DRAFT_4163 0.58 -0.2
145,590 - EX28DRAFT_4163 0.58 -0.0

Or see this region's nucleotide sequence