Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4153 and EX28DRAFT_4154 are separated by 92 nucleotides EX28DRAFT_4154 and EX28DRAFT_4155 are separated by 67 nucleotides EX28DRAFT_4155 and EX28DRAFT_4156 are separated by 41 nucleotides
EX28DRAFT_4153: EX28DRAFT_4153 - 3-dehydroquinate synthase (EC 4.2.3.4), at 133,910 to 134,998
_4153
EX28DRAFT_4154: EX28DRAFT_4154 - Uncharacterized protein conserved in bacteria, at 135,091 to 136,407
_4154
EX28DRAFT_4155: EX28DRAFT_4155 - DNA adenine methylase (dam), at 136,475 to 137,287
_4155
EX28DRAFT_4156: EX28DRAFT_4156 - ribulose-phosphate 3-epimerase, at 137,329 to 138,006
_4156
Position (kb) 135
136
137 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 134.479 kb on + strand, within EX28DRAFT_4153 at 134.518 kb on + strand, within EX28DRAFT_4153 at 134.519 kb on - strand, within EX28DRAFT_4153 at 134.650 kb on + strand, within EX28DRAFT_4153 at 134.852 kb on + strand, within EX28DRAFT_4153 at 135.068 kb on + strand at 135.379 kb on - strand, within EX28DRAFT_4154 at 135.671 kb on + strand, within EX28DRAFT_4154 at 135.867 kb on + strand, within EX28DRAFT_4154 at 135.867 kb on + strand, within EX28DRAFT_4154 at 136.162 kb on + strand, within EX28DRAFT_4154 at 136.192 kb on + strand, within EX28DRAFT_4154 at 136.192 kb on + strand, within EX28DRAFT_4154 at 136.276 kb on + strand at 136.276 kb on + strand at 136.297 kb on + strand at 136.297 kb on + strand at 136.300 kb on - strand at 136.300 kb on - strand at 136.406 kb on - strand at 136.459 kb on + strand at 136.459 kb on + strand at 136.537 kb on + strand at 136.624 kb on + strand, within EX28DRAFT_4155 at 136.847 kb on + strand, within EX28DRAFT_4155 at 136.979 kb on - strand, within EX28DRAFT_4155 at 137.009 kb on + strand, within EX28DRAFT_4155 at 137.171 kb on - strand, within EX28DRAFT_4155 at 137.183 kb on + strand, within EX28DRAFT_4155 at 137.222 kb on - strand at 137.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 134,479 + EX28DRAFT_4153 0.52 +1.9 134,518 + EX28DRAFT_4153 0.56 -2.4 134,519 - EX28DRAFT_4153 0.56 -2.9 134,650 + EX28DRAFT_4153 0.68 -0.4 134,852 + EX28DRAFT_4153 0.87 -1.2 135,068 + +0.1 135,379 - EX28DRAFT_4154 0.22 -2.0 135,671 + EX28DRAFT_4154 0.44 -0.4 135,867 + EX28DRAFT_4154 0.59 -0.3 135,867 + EX28DRAFT_4154 0.59 -0.7 136,162 + EX28DRAFT_4154 0.81 -0.0 136,192 + EX28DRAFT_4154 0.84 -0.7 136,192 + EX28DRAFT_4154 0.84 -0.2 136,276 + -0.2 136,276 + +0.3 136,297 + +0.3 136,297 + -0.1 136,300 - -0.7 136,300 - -2.3 136,406 - -2.2 136,459 + -2.5 136,459 + +0.5 136,537 + -2.4 136,624 + EX28DRAFT_4155 0.18 -1.7 136,847 + EX28DRAFT_4155 0.46 -2.9 136,979 - EX28DRAFT_4155 0.62 -3.5 137,009 + EX28DRAFT_4155 0.66 -4.3 137,171 - EX28DRAFT_4155 0.86 -2.4 137,183 + EX28DRAFT_4155 0.87 -5.1 137,222 - -4.5 137,228 - -1.4
Or see this region's nucleotide sequence