Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2853 and EX28DRAFT_2854 are separated by 306 nucleotides EX28DRAFT_2854 and EX28DRAFT_2855 are separated by 85 nucleotides EX28DRAFT_2855 and EX28DRAFT_2856 are separated by 54 nucleotides
EX28DRAFT_2853: EX28DRAFT_2853 - sulfite reductase [NADPH] flavoprotein, alpha-component, at 154,670 to 156,475
_2853
EX28DRAFT_2854: EX28DRAFT_2854 - queuosine biosynthesis protein QueD, at 156,782 to 157,144
_2854
EX28DRAFT_2855: EX28DRAFT_2855 - putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE, at 157,230 to 157,901
_2855
EX28DRAFT_2856: EX28DRAFT_2856 - Putative N-acetylmannosamine-6-phosphate epimerase, at 157,956 to 158,663
_2856
Position (kb) 156
157
158 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 156.003 kb on + strand, within EX28DRAFT_2853 at 156.173 kb on - strand, within EX28DRAFT_2853 at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.555 kb on - strand at 156.561 kb on + strand at 156.561 kb on + strand at 156.578 kb on + strand at 156.585 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.617 kb on + strand at 156.618 kb on - strand at 156.718 kb on + strand at 156.746 kb on + strand at 156.746 kb on + strand at 156.853 kb on + strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.974 kb on + strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 157.127 kb on - strand at 157.127 kb on - strand at 157.130 kb on + strand at 157.239 kb on - strand at 157.338 kb on + strand, within EX28DRAFT_2855 at 157.427 kb on + strand, within EX28DRAFT_2855 at 157.461 kb on + strand, within EX28DRAFT_2855 at 157.462 kb on - strand, within EX28DRAFT_2855 at 157.731 kb on + strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.818 kb on + strand, within EX28DRAFT_2855 at 157.852 kb on + strand at 157.852 kb on + strand at 157.852 kb on + strand at 157.902 kb on - strand at 157.931 kb on - strand at 157.934 kb on - strand at 157.994 kb on + strand at 157.995 kb on - strand at 158.007 kb on - strand at 158.049 kb on - strand, within EX28DRAFT_2856
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 156,003 + EX28DRAFT_2853 0.74 -5.0 156,173 - EX28DRAFT_2853 0.83 -2.7 156,533 + -1.4 156,533 + -3.3 156,534 - -1.4 156,555 - +0.2 156,561 + -0.5 156,561 + -0.1 156,578 + -0.2 156,585 + -3.4 156,608 + +0.5 156,609 - -0.8 156,617 + -0.4 156,618 - -0.2 156,718 + -1.5 156,746 + -0.0 156,746 + +1.6 156,853 + EX28DRAFT_2854 0.20 -0.0 156,854 - EX28DRAFT_2854 0.20 +0.5 156,854 - EX28DRAFT_2854 0.20 +0.8 156,974 + EX28DRAFT_2854 0.53 -0.2 156,975 - EX28DRAFT_2854 0.53 +0.9 156,975 - EX28DRAFT_2854 0.53 -0.4 157,127 - -0.1 157,127 - -1.4 157,130 + -1.1 157,239 - +1.1 157,338 + EX28DRAFT_2855 0.16 -0.9 157,427 + EX28DRAFT_2855 0.29 +0.6 157,461 + EX28DRAFT_2855 0.34 +0.0 157,462 - EX28DRAFT_2855 0.35 +0.0 157,731 + EX28DRAFT_2855 0.75 -0.0 157,737 - EX28DRAFT_2855 0.75 +0.0 157,737 - EX28DRAFT_2855 0.75 -0.0 157,737 - EX28DRAFT_2855 0.75 -0.0 157,775 + EX28DRAFT_2855 0.81 +0.0 157,775 + EX28DRAFT_2855 0.81 -0.1 157,775 + EX28DRAFT_2855 0.81 +0.5 157,776 - EX28DRAFT_2855 0.81 +0.8 157,776 - EX28DRAFT_2855 0.81 -0.7 157,818 + EX28DRAFT_2855 0.88 +0.3 157,852 + -0.9 157,852 + -0.4 157,852 + +0.2 157,902 - +0.4 157,931 - -0.7 157,934 - +0.2 157,994 + -0.3 157,995 - +0.8 158,007 - -0.1 158,049 - EX28DRAFT_2856 0.13 -0.6
Or see this region's nucleotide sequence