Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2204 and EX28DRAFT_2205 are separated by 64 nucleotides EX28DRAFT_2205 and EX28DRAFT_2206 are separated by 1 nucleotides EX28DRAFT_2206 and EX28DRAFT_2207 overlap by 8 nucleotides EX28DRAFT_2207 and EX28DRAFT_2208 are separated by 3 nucleotides EX28DRAFT_2208 and EX28DRAFT_2209 are separated by 24 nucleotides
EX28DRAFT_2204: EX28DRAFT_2204 - Integral membrane protein, interacts with FtsH, at 2,228,312 to 2,229,019
_2204
EX28DRAFT_2205: EX28DRAFT_2205 - Molybdopterin converting factor, large subunit, at 2,229,084 to 2,229,536
_2205
EX28DRAFT_2206: EX28DRAFT_2206 - molybdopterin converting factor, subunit 1, non-archaeal, at 2,229,538 to 2,229,783
_2206
EX28DRAFT_2207: EX28DRAFT_2207 - molybdenum cofactor biosynthesis protein MoaC, at 2,229,776 to 2,230,261
_2207
EX28DRAFT_2208: EX28DRAFT_2208 - molybdenum cofactor biosynthesis protein B, proteobacterial, at 2,230,265 to 2,230,777
_2208
EX28DRAFT_2209: EX28DRAFT_2209 - molybdenum cofactor biosynthesis protein A, bacterial, at 2,230,802 to 2,231,791
_2209
Position (kb)
2229
2230
2231 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2228.896 kb on - strand, within EX28DRAFT_2204 at 2228.956 kb on + strand at 2228.956 kb on + strand at 2228.957 kb on - strand at 2228.957 kb on - strand at 2228.958 kb on + strand at 2228.958 kb on + strand at 2228.958 kb on + strand at 2228.959 kb on - strand at 2228.959 kb on - strand at 2228.987 kb on + strand at 2228.990 kb on + strand at 2228.991 kb on - strand at 2229.041 kb on - strand at 2229.041 kb on - strand at 2229.041 kb on - strand at 2229.041 kb on - strand at 2229.044 kb on + strand at 2229.044 kb on + strand at 2229.057 kb on + strand at 2229.188 kb on - strand, within EX28DRAFT_2205 at 2229.195 kb on - strand, within EX28DRAFT_2205 at 2229.481 kb on + strand, within EX28DRAFT_2205 at 2229.492 kb on + strand at 2229.778 kb on - strand at 2229.796 kb on + strand at 2229.853 kb on + strand, within EX28DRAFT_2207 at 2229.853 kb on + strand, within EX28DRAFT_2207 at 2229.853 kb on + strand, within EX28DRAFT_2207 at 2229.854 kb on - strand, within EX28DRAFT_2207 at 2229.883 kb on - strand, within EX28DRAFT_2207 at 2229.883 kb on - strand, within EX28DRAFT_2207 at 2229.947 kb on + strand, within EX28DRAFT_2207 at 2230.220 kb on - strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.261 kb on + strand at 2230.262 kb on - strand at 2230.262 kb on - strand at 2230.262 kb on - strand at 2230.262 kb on - strand at 2230.266 kb on + strand at 2230.266 kb on + strand at 2230.267 kb on - strand at 2230.267 kb on - strand at 2230.291 kb on + strand at 2230.481 kb on + strand, within EX28DRAFT_2208 at 2230.481 kb on + strand, within EX28DRAFT_2208 at 2230.697 kb on - strand, within EX28DRAFT_2208 at 2230.697 kb on - strand, within EX28DRAFT_2208 at 2230.735 kb on + strand at 2230.777 kb on + strand at 2230.778 kb on - strand at 2230.778 kb on - strand at 2230.832 kb on - strand at 2230.834 kb on + strand at 2230.949 kb on + strand, within EX28DRAFT_2209 at 2230.949 kb on + strand, within EX28DRAFT_2209 at 2231.038 kb on - strand, within EX28DRAFT_2209 at 2231.047 kb on + strand, within EX28DRAFT_2209 at 2231.047 kb on + strand, within EX28DRAFT_2209 at 2231.047 kb on + strand, within EX28DRAFT_2209 at 2231.047 kb on + strand, within EX28DRAFT_2209 at 2231.048 kb on - strand, within EX28DRAFT_2209 at 2231.221 kb on + strand, within EX28DRAFT_2209
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,228,896 - EX28DRAFT_2204 0.82 +1.6 2,228,956 + +0.4 2,228,956 + +0.6 2,228,957 - +0.4 2,228,957 - -0.2 2,228,958 + -0.1 2,228,958 + -0.3 2,228,958 + +0.1 2,228,959 - +0.1 2,228,959 - -0.7 2,228,987 + -0.1 2,228,990 + -0.9 2,228,991 - -0.1 2,229,041 - -0.2 2,229,041 - -0.4 2,229,041 - -0.1 2,229,041 - +0.6 2,229,044 + -0.9 2,229,044 + -0.0 2,229,057 + -0.4 2,229,188 - EX28DRAFT_2205 0.23 +0.3 2,229,195 - EX28DRAFT_2205 0.25 -0.2 2,229,481 + EX28DRAFT_2205 0.88 +0.5 2,229,492 + +0.5 2,229,778 - -0.8 2,229,796 + +0.3 2,229,853 + EX28DRAFT_2207 0.16 -0.3 2,229,853 + EX28DRAFT_2207 0.16 +1.8 2,229,853 + EX28DRAFT_2207 0.16 -0.5 2,229,854 - EX28DRAFT_2207 0.16 -0.1 2,229,883 - EX28DRAFT_2207 0.22 -0.1 2,229,883 - EX28DRAFT_2207 0.22 +0.2 2,229,947 + EX28DRAFT_2207 0.35 -0.1 2,230,220 - +0.4 2,230,261 + +0.1 2,230,261 + -0.3 2,230,261 + +1.0 2,230,261 + -0.9 2,230,261 + -0.4 2,230,261 + -0.1 2,230,261 + -0.0 2,230,261 + -0.0 2,230,262 - +0.7 2,230,262 - -0.0 2,230,262 - +0.1 2,230,262 - -1.3 2,230,266 + -0.4 2,230,266 + +0.1 2,230,267 - +0.0 2,230,267 - -1.6 2,230,291 + +0.7 2,230,481 + EX28DRAFT_2208 0.42 +0.1 2,230,481 + EX28DRAFT_2208 0.42 -1.6 2,230,697 - EX28DRAFT_2208 0.84 +0.0 2,230,697 - EX28DRAFT_2208 0.84 -0.8 2,230,735 + -0.3 2,230,777 + -1.6 2,230,778 - -0.0 2,230,778 - +0.2 2,230,832 - +0.4 2,230,834 + -0.5 2,230,949 + EX28DRAFT_2209 0.15 -0.5 2,230,949 + EX28DRAFT_2209 0.15 +1.3 2,231,038 - EX28DRAFT_2209 0.24 -3.1 2,231,047 + EX28DRAFT_2209 0.25 +1.0 2,231,047 + EX28DRAFT_2209 0.25 -1.5 2,231,047 + EX28DRAFT_2209 0.25 +0.4 2,231,047 + EX28DRAFT_2209 0.25 +0.5 2,231,048 - EX28DRAFT_2209 0.25 -0.0 2,231,221 + EX28DRAFT_2209 0.42 +0.1
Or see this region's nucleotide sequence