Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2094 and EX28DRAFT_2095 are separated by 40 nucleotides EX28DRAFT_2095 and EX28DRAFT_2096 are separated by 65 nucleotides EX28DRAFT_2096 and EX28DRAFT_2097 overlap by 4 nucleotides EX28DRAFT_2097 and EX28DRAFT_2098 are separated by 9 nucleotides
EX28DRAFT_2094: EX28DRAFT_2094 - 23S rRNA (uracil-5-)-methyltransferase RumB, at 2,118,149 to 2,119,279
_2094
EX28DRAFT_2095: EX28DRAFT_2095 - Protein of unknown function (DUF2593), at 2,119,320 to 2,119,793
_2095
EX28DRAFT_2096: EX28DRAFT_2096 - ABC-type spermidine/putrescine transport system, permease component II, at 2,119,859 to 2,120,704
_2096
EX28DRAFT_2097: EX28DRAFT_2097 - ABC-type spermidine/putrescine transport system, permease component I, at 2,120,701 to 2,121,654
_2097
EX28DRAFT_2098: EX28DRAFT_2098 - spermidine/putrescine ABC transporter ATP-binding subunit, at 2,121,664 to 2,122,797
_2098
Position (kb)
2119
2120
2121 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2119.108 kb on + strand, within EX28DRAFT_2094 at 2119.229 kb on + strand at 2119.263 kb on + strand at 2119.280 kb on - strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.315 kb on - strand at 2119.321 kb on + strand at 2119.489 kb on + strand, within EX28DRAFT_2095 at 2119.709 kb on + strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.712 kb on + strand, within EX28DRAFT_2095 at 2119.746 kb on + strand at 2119.746 kb on + strand at 2119.747 kb on - strand at 2119.747 kb on - strand at 2119.752 kb on - strand at 2119.766 kb on + strand at 2119.766 kb on + strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.770 kb on - strand at 2119.799 kb on + strand at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.964 kb on - strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.245 kb on - strand, within EX28DRAFT_2096 at 2120.252 kb on - strand, within EX28DRAFT_2096 at 2120.393 kb on - strand, within EX28DRAFT_2096 at 2120.550 kb on + strand, within EX28DRAFT_2096 at 2120.551 kb on - strand, within EX28DRAFT_2096 at 2120.608 kb on - strand, within EX28DRAFT_2096 at 2120.608 kb on - strand, within EX28DRAFT_2096 at 2120.635 kb on - strand at 2120.694 kb on - strand at 2121.021 kb on - strand, within EX28DRAFT_2097 at 2121.021 kb on - strand, within EX28DRAFT_2097 at 2121.071 kb on + strand, within EX28DRAFT_2097 at 2121.077 kb on + strand, within EX28DRAFT_2097 at 2121.077 kb on + strand, within EX28DRAFT_2097 at 2121.077 kb on + strand, within EX28DRAFT_2097 at 2121.078 kb on - strand, within EX28DRAFT_2097 at 2121.093 kb on - strand, within EX28DRAFT_2097 at 2121.093 kb on - strand, within EX28DRAFT_2097 at 2121.256 kb on - strand, within EX28DRAFT_2097 at 2121.259 kb on - strand, within EX28DRAFT_2097 at 2121.259 kb on - strand, within EX28DRAFT_2097 at 2121.372 kb on - strand, within EX28DRAFT_2097 at 2121.456 kb on + strand, within EX28DRAFT_2097 at 2121.493 kb on + strand, within EX28DRAFT_2097 at 2121.545 kb on + strand, within EX28DRAFT_2097 at 2121.666 kb on - strand at 2121.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 2,119,108 + EX28DRAFT_2094 0.85 -0.2 2,119,229 + +0.3 2,119,263 + -0.5 2,119,280 - -2.3 2,119,314 + -0.5 2,119,314 + -0.7 2,119,314 + +1.3 2,119,315 - +0.2 2,119,321 + -0.8 2,119,489 + EX28DRAFT_2095 0.36 -0.2 2,119,709 + EX28DRAFT_2095 0.82 +0.2 2,119,710 - EX28DRAFT_2095 0.82 +0.6 2,119,710 - EX28DRAFT_2095 0.82 +0.5 2,119,710 - EX28DRAFT_2095 0.82 +0.4 2,119,712 + EX28DRAFT_2095 0.83 +0.9 2,119,746 + +0.3 2,119,746 + +0.6 2,119,747 - -0.4 2,119,747 - +0.9 2,119,752 - +0.7 2,119,766 + +0.1 2,119,766 + -0.0 2,119,767 - -0.2 2,119,767 - -0.5 2,119,767 - -0.1 2,119,770 - -2.7 2,119,799 + -0.4 2,119,963 + EX28DRAFT_2096 0.12 -2.0 2,119,963 + EX28DRAFT_2096 0.12 +0.4 2,119,964 - EX28DRAFT_2096 0.12 -2.2 2,120,244 + EX28DRAFT_2096 0.46 +0.3 2,120,244 + EX28DRAFT_2096 0.46 +2.5 2,120,245 - EX28DRAFT_2096 0.46 +0.0 2,120,252 - EX28DRAFT_2096 0.46 -0.5 2,120,393 - EX28DRAFT_2096 0.63 -1.4 2,120,550 + EX28DRAFT_2096 0.82 +0.4 2,120,551 - EX28DRAFT_2096 0.82 -0.4 2,120,608 - EX28DRAFT_2096 0.89 -1.2 2,120,608 - EX28DRAFT_2096 0.89 +0.3 2,120,635 - -0.3 2,120,694 - -0.1 2,121,021 - EX28DRAFT_2097 0.34 -0.5 2,121,021 - EX28DRAFT_2097 0.34 +0.0 2,121,071 + EX28DRAFT_2097 0.39 -0.2 2,121,077 + EX28DRAFT_2097 0.39 +0.7 2,121,077 + EX28DRAFT_2097 0.39 +0.3 2,121,077 + EX28DRAFT_2097 0.39 -0.4 2,121,078 - EX28DRAFT_2097 0.40 +0.1 2,121,093 - EX28DRAFT_2097 0.41 -4.1 2,121,093 - EX28DRAFT_2097 0.41 +0.5 2,121,256 - EX28DRAFT_2097 0.58 +0.1 2,121,259 - EX28DRAFT_2097 0.58 -0.7 2,121,259 - EX28DRAFT_2097 0.58 +0.2 2,121,372 - EX28DRAFT_2097 0.70 -0.1 2,121,456 + EX28DRAFT_2097 0.79 -1.6 2,121,493 + EX28DRAFT_2097 0.83 -1.0 2,121,545 + EX28DRAFT_2097 0.88 -0.2 2,121,666 - +0.0 2,121,666 - -2.3
Or see this region's nucleotide sequence