Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1923 and EX28DRAFT_1924 are separated by 20 nucleotides EX28DRAFT_1924 and EX28DRAFT_1925 are separated by 33 nucleotides EX28DRAFT_1925 and EX28DRAFT_1926 are separated by 152 nucleotides EX28DRAFT_1926 and EX28DRAFT_1927 are separated by 125 nucleotides
EX28DRAFT_1923: EX28DRAFT_1923 - NAD(P)H:quinone oxidoreductase, type IV, at 1,932,489 to 1,933,085
_1923
EX28DRAFT_1924: EX28DRAFT_1924 - YccJ-like protein, at 1,933,106 to 1,933,333
_1924
EX28DRAFT_1925: EX28DRAFT_1925 - Histidine phosphatase superfamily (branch 2), at 1,933,367 to 1,934,608
_1925
EX28DRAFT_1926: EX28DRAFT_1926 - transcriptional regulator, HxlR family, at 1,934,761 to 1,935,204
_1926
EX28DRAFT_1927: EX28DRAFT_1927 - Short-chain dehydrogenases of various substrate specificities, at 1,935,330 to 1,936,124
_1927
Position (kb)
1933
1934
1935 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1932.431 kb on + strand at 1932.431 kb on + strand at 1932.432 kb on - strand at 1932.432 kb on - strand at 1932.432 kb on - strand at 1932.513 kb on + strand at 1932.513 kb on + strand at 1932.522 kb on + strand at 1932.522 kb on + strand at 1932.522 kb on + strand at 1932.522 kb on + strand at 1932.522 kb on + strand at 1932.523 kb on - strand at 1932.523 kb on - strand at 1932.523 kb on - strand at 1932.523 kb on - strand at 1932.523 kb on - strand at 1932.735 kb on - strand, within EX28DRAFT_1923 at 1932.735 kb on - strand, within EX28DRAFT_1923 at 1932.818 kb on - strand, within EX28DRAFT_1923 at 1932.818 kb on - strand, within EX28DRAFT_1923 at 1932.860 kb on + strand, within EX28DRAFT_1923 at 1932.860 kb on + strand, within EX28DRAFT_1923 at 1932.967 kb on - strand, within EX28DRAFT_1923 at 1933.050 kb on + strand at 1933.050 kb on + strand at 1933.051 kb on - strand at 1933.205 kb on + strand, within EX28DRAFT_1924 at 1933.528 kb on - strand, within EX28DRAFT_1925 at 1933.629 kb on + strand, within EX28DRAFT_1925 at 1933.630 kb on - strand, within EX28DRAFT_1925 at 1933.630 kb on - strand, within EX28DRAFT_1925 at 1933.732 kb on + strand, within EX28DRAFT_1925 at 1933.740 kb on + strand, within EX28DRAFT_1925 at 1933.787 kb on - strand, within EX28DRAFT_1925 at 1933.890 kb on + strand, within EX28DRAFT_1925 at 1933.890 kb on + strand, within EX28DRAFT_1925 at 1933.893 kb on + strand, within EX28DRAFT_1925 at 1933.981 kb on + strand, within EX28DRAFT_1925 at 1934.040 kb on + strand, within EX28DRAFT_1925 at 1934.065 kb on + strand, within EX28DRAFT_1925 at 1934.065 kb on + strand, within EX28DRAFT_1925 at 1934.260 kb on + strand, within EX28DRAFT_1925 at 1934.355 kb on + strand, within EX28DRAFT_1925 at 1934.355 kb on + strand, within EX28DRAFT_1925 at 1934.356 kb on - strand, within EX28DRAFT_1925 at 1934.356 kb on - strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.368 kb on - strand, within EX28DRAFT_1925 at 1934.507 kb on + strand at 1934.507 kb on + strand at 1934.508 kb on - strand at 1934.523 kb on + strand at 1934.612 kb on + strand at 1934.613 kb on - strand at 1934.714 kb on + strand at 1934.770 kb on - strand at 1934.998 kb on + strand, within EX28DRAFT_1926 at 1934.998 kb on + strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1935.322 kb on - strand at 1935.581 kb on + strand, within EX28DRAFT_1927
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,932,431 + -0.1 1,932,431 + +0.4 1,932,432 - +0.4 1,932,432 - +1.1 1,932,432 - +0.2 1,932,513 + -0.3 1,932,513 + -0.8 1,932,522 + -0.2 1,932,522 + -0.9 1,932,522 + -2.2 1,932,522 + +0.2 1,932,522 + -0.4 1,932,523 - -0.4 1,932,523 - +1.5 1,932,523 - +0.5 1,932,523 - -0.0 1,932,523 - +0.9 1,932,735 - EX28DRAFT_1923 0.41 +1.5 1,932,735 - EX28DRAFT_1923 0.41 +1.4 1,932,818 - EX28DRAFT_1923 0.55 +0.0 1,932,818 - EX28DRAFT_1923 0.55 +0.2 1,932,860 + EX28DRAFT_1923 0.62 -1.4 1,932,860 + EX28DRAFT_1923 0.62 -1.3 1,932,967 - EX28DRAFT_1923 0.80 +1.8 1,933,050 + -0.5 1,933,050 + +0.1 1,933,051 - +1.1 1,933,205 + EX28DRAFT_1924 0.43 -0.1 1,933,528 - EX28DRAFT_1925 0.13 +0.2 1,933,629 + EX28DRAFT_1925 0.21 -3.2 1,933,630 - EX28DRAFT_1925 0.21 +0.0 1,933,630 - EX28DRAFT_1925 0.21 +0.3 1,933,732 + EX28DRAFT_1925 0.29 -1.7 1,933,740 + EX28DRAFT_1925 0.30 -0.1 1,933,787 - EX28DRAFT_1925 0.34 +0.9 1,933,890 + EX28DRAFT_1925 0.42 -0.0 1,933,890 + EX28DRAFT_1925 0.42 +0.4 1,933,893 + EX28DRAFT_1925 0.42 -1.0 1,933,981 + EX28DRAFT_1925 0.49 -0.8 1,934,040 + EX28DRAFT_1925 0.54 +0.7 1,934,065 + EX28DRAFT_1925 0.56 -0.4 1,934,065 + EX28DRAFT_1925 0.56 +0.0 1,934,260 + EX28DRAFT_1925 0.72 +0.7 1,934,355 + EX28DRAFT_1925 0.80 -0.1 1,934,355 + EX28DRAFT_1925 0.80 +0.2 1,934,356 - EX28DRAFT_1925 0.80 -0.5 1,934,356 - EX28DRAFT_1925 0.80 -1.3 1,934,367 + EX28DRAFT_1925 0.81 -0.0 1,934,367 + EX28DRAFT_1925 0.81 -0.1 1,934,367 + EX28DRAFT_1925 0.81 -0.1 1,934,367 + EX28DRAFT_1925 0.81 -0.4 1,934,368 - EX28DRAFT_1925 0.81 -3.7 1,934,507 + +1.9 1,934,507 + +0.5 1,934,508 - -0.1 1,934,523 + -0.6 1,934,612 + -1.7 1,934,613 - -0.1 1,934,714 + -0.2 1,934,770 - +0.1 1,934,998 + EX28DRAFT_1926 0.53 +0.8 1,934,998 + EX28DRAFT_1926 0.53 +0.8 1,934,999 - EX28DRAFT_1926 0.54 -0.1 1,934,999 - EX28DRAFT_1926 0.54 -0.3 1,934,999 - EX28DRAFT_1926 0.54 +0.3 1,935,322 - +0.1 1,935,581 + EX28DRAFT_1927 0.32 -1.3
Or see this region's nucleotide sequence