Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1765 and EX28DRAFT_1766 are separated by 116 nucleotides EX28DRAFT_1766 and EX28DRAFT_1767 are separated by 167 nucleotides EX28DRAFT_1767 and EX28DRAFT_1768 are separated by 63 nucleotides
EX28DRAFT_1765: EX28DRAFT_1765 - selenophosphate synthase (EC 2.7.9.3), at 1,780,365 to 1,781,408
_1765
EX28DRAFT_1766: EX28DRAFT_1766 - Nitroreductase, at 1,781,525 to 1,782,076
_1766
EX28DRAFT_1767: EX28DRAFT_1767 - signal peptide peptidase SppA, 67K type, at 1,782,244 to 1,784,100
_1767
EX28DRAFT_1768: EX28DRAFT_1768 - L-asparaginase, type I, at 1,784,164 to 1,785,180
_1768
Position (kb)
1782
1783
1784
1785 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1781.377 kb on + strand at 1781.441 kb on + strand at 1781.463 kb on + strand at 1781.464 kb on - strand at 1781.464 kb on - strand at 1781.467 kb on + strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.468 kb on - strand at 1781.582 kb on - strand, within EX28DRAFT_1766 at 1781.620 kb on + strand, within EX28DRAFT_1766 at 1781.930 kb on - strand, within EX28DRAFT_1766 at 1782.180 kb on - strand at 1782.196 kb on - strand at 1782.241 kb on + strand at 1782.241 kb on + strand at 1782.241 kb on + strand at 1782.242 kb on - strand at 1782.279 kb on + strand at 1782.376 kb on - strand at 1782.722 kb on - strand, within EX28DRAFT_1767 at 1782.805 kb on + strand, within EX28DRAFT_1767 at 1782.805 kb on + strand, within EX28DRAFT_1767 at 1782.808 kb on + strand, within EX28DRAFT_1767 at 1782.866 kb on - strand, within EX28DRAFT_1767 at 1782.867 kb on + strand, within EX28DRAFT_1767 at 1782.867 kb on + strand, within EX28DRAFT_1767 at 1782.868 kb on - strand, within EX28DRAFT_1767 at 1782.900 kb on + strand, within EX28DRAFT_1767 at 1782.958 kb on + strand, within EX28DRAFT_1767 at 1782.990 kb on + strand, within EX28DRAFT_1767 at 1783.042 kb on - strand, within EX28DRAFT_1767 at 1783.158 kb on + strand, within EX28DRAFT_1767 at 1783.183 kb on + strand, within EX28DRAFT_1767 at 1783.183 kb on + strand, within EX28DRAFT_1767 at 1783.183 kb on + strand, within EX28DRAFT_1767 at 1783.183 kb on + strand, within EX28DRAFT_1767 at 1783.184 kb on - strand, within EX28DRAFT_1767 at 1783.184 kb on - strand, within EX28DRAFT_1767 at 1783.189 kb on + strand, within EX28DRAFT_1767 at 1783.501 kb on + strand, within EX28DRAFT_1767 at 1783.501 kb on + strand, within EX28DRAFT_1767 at 1783.502 kb on - strand, within EX28DRAFT_1767 at 1783.696 kb on + strand, within EX28DRAFT_1767 at 1783.697 kb on - strand, within EX28DRAFT_1767 at 1783.698 kb on + strand, within EX28DRAFT_1767 at 1783.708 kb on - strand, within EX28DRAFT_1767 at 1783.888 kb on + strand, within EX28DRAFT_1767 at 1783.891 kb on + strand, within EX28DRAFT_1767 at 1783.979 kb on - strand at 1783.979 kb on - strand at 1784.186 kb on + strand at 1784.233 kb on + strand at 1784.233 kb on + strand at 1784.234 kb on - strand at 1784.235 kb on + strand at 1784.389 kb on - strand, within EX28DRAFT_1768 at 1784.573 kb on - strand, within EX28DRAFT_1768 at 1784.811 kb on + strand, within EX28DRAFT_1768 at 1784.811 kb on + strand, within EX28DRAFT_1768 at 1784.811 kb on + strand, within EX28DRAFT_1768 at 1784.811 kb on + strand, within EX28DRAFT_1768 at 1784.811 kb on + strand, within EX28DRAFT_1768 at 1784.815 kb on + strand, within EX28DRAFT_1768 at 1784.845 kb on - strand, within EX28DRAFT_1768 at 1784.898 kb on + strand, within EX28DRAFT_1768 at 1784.898 kb on + strand, within EX28DRAFT_1768 at 1784.982 kb on + strand, within EX28DRAFT_1768 at 1784.983 kb on - strand, within EX28DRAFT_1768 at 1784.983 kb on - strand, within EX28DRAFT_1768 at 1784.983 kb on - strand, within EX28DRAFT_1768
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 1,781,377 + +0.2 1,781,441 + -2.6 1,781,463 + -1.0 1,781,464 - +0.3 1,781,464 - +1.2 1,781,467 + +1.0 1,781,468 - -0.6 1,781,468 - +0.5 1,781,468 - +0.4 1,781,468 - -0.6 1,781,582 - EX28DRAFT_1766 0.10 -0.3 1,781,620 + EX28DRAFT_1766 0.17 -0.1 1,781,930 - EX28DRAFT_1766 0.73 +0.0 1,782,180 - +0.4 1,782,196 - +0.4 1,782,241 + +0.3 1,782,241 + +0.9 1,782,241 + +0.0 1,782,242 - +1.1 1,782,279 + -0.8 1,782,376 - -0.2 1,782,722 - EX28DRAFT_1767 0.26 +0.2 1,782,805 + EX28DRAFT_1767 0.30 -0.2 1,782,805 + EX28DRAFT_1767 0.30 +0.1 1,782,808 + EX28DRAFT_1767 0.30 +0.7 1,782,866 - EX28DRAFT_1767 0.33 -0.9 1,782,867 + EX28DRAFT_1767 0.34 +0.7 1,782,867 + EX28DRAFT_1767 0.34 -0.1 1,782,868 - EX28DRAFT_1767 0.34 +0.3 1,782,900 + EX28DRAFT_1767 0.35 +0.1 1,782,958 + EX28DRAFT_1767 0.38 -0.2 1,782,990 + EX28DRAFT_1767 0.40 +0.7 1,783,042 - EX28DRAFT_1767 0.43 +0.6 1,783,158 + EX28DRAFT_1767 0.49 +0.1 1,783,183 + EX28DRAFT_1767 0.51 +0.7 1,783,183 + EX28DRAFT_1767 0.51 -1.7 1,783,183 + EX28DRAFT_1767 0.51 -0.1 1,783,183 + EX28DRAFT_1767 0.51 -0.1 1,783,184 - EX28DRAFT_1767 0.51 +0.9 1,783,184 - EX28DRAFT_1767 0.51 -0.8 1,783,189 + EX28DRAFT_1767 0.51 -1.1 1,783,501 + EX28DRAFT_1767 0.68 +0.2 1,783,501 + EX28DRAFT_1767 0.68 -0.3 1,783,502 - EX28DRAFT_1767 0.68 +0.8 1,783,696 + EX28DRAFT_1767 0.78 +1.0 1,783,697 - EX28DRAFT_1767 0.78 -0.8 1,783,698 + EX28DRAFT_1767 0.78 +1.5 1,783,708 - EX28DRAFT_1767 0.79 -0.0 1,783,888 + EX28DRAFT_1767 0.89 +0.1 1,783,891 + EX28DRAFT_1767 0.89 -0.4 1,783,979 - +0.4 1,783,979 - -0.6 1,784,186 + +0.8 1,784,233 + -0.2 1,784,233 + -0.4 1,784,234 - +0.0 1,784,235 + +0.1 1,784,389 - EX28DRAFT_1768 0.22 -0.1 1,784,573 - EX28DRAFT_1768 0.40 +0.6 1,784,811 + EX28DRAFT_1768 0.64 +0.7 1,784,811 + EX28DRAFT_1768 0.64 +0.8 1,784,811 + EX28DRAFT_1768 0.64 -0.7 1,784,811 + EX28DRAFT_1768 0.64 +0.6 1,784,811 + EX28DRAFT_1768 0.64 -0.4 1,784,815 + EX28DRAFT_1768 0.64 -0.1 1,784,845 - EX28DRAFT_1768 0.67 -1.3 1,784,898 + EX28DRAFT_1768 0.72 +0.5 1,784,898 + EX28DRAFT_1768 0.72 -0.6 1,784,982 + EX28DRAFT_1768 0.80 +0.7 1,784,983 - EX28DRAFT_1768 0.81 +3.4 1,784,983 - EX28DRAFT_1768 0.81 +0.8 1,784,983 - EX28DRAFT_1768 0.81 +1.0
Or see this region's nucleotide sequence