Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0312 and EX28DRAFT_0313 are separated by 76 nucleotides EX28DRAFT_0313 and EX28DRAFT_0314 are separated by 337 nucleotides EX28DRAFT_0314 and EX28DRAFT_0315 are separated by 92 nucleotides
EX28DRAFT_0312: EX28DRAFT_0312 - phosphate acetyltransferase, at 270,741 to 272,882
_0312
EX28DRAFT_0313: EX28DRAFT_0313 - acetate kinase, at 272,959 to 274,161
_0313
EX28DRAFT_0314: EX28DRAFT_0314 - Uncharacterized protein conserved in bacteria, at 274,499 to 274,954
_0314
EX28DRAFT_0315: EX28DRAFT_0315 - Uncharacterized conserved protein, at 275,047 to 275,541
_0315
Position (kb) 272
273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 272.603 kb on - strand, within EX28DRAFT_0312 at 272.868 kb on - strand at 272.868 kb on - strand at 272.871 kb on - strand at 273.212 kb on + strand, within EX28DRAFT_0313 at 273.212 kb on + strand, within EX28DRAFT_0313 at 273.284 kb on + strand, within EX28DRAFT_0313 at 273.285 kb on - strand, within EX28DRAFT_0313 at 273.299 kb on + strand, within EX28DRAFT_0313 at 273.481 kb on - strand, within EX28DRAFT_0313 at 273.599 kb on + strand, within EX28DRAFT_0313 at 273.599 kb on + strand, within EX28DRAFT_0313 at 273.600 kb on - strand, within EX28DRAFT_0313 at 273.678 kb on - strand, within EX28DRAFT_0313 at 273.678 kb on - strand, within EX28DRAFT_0313 at 273.954 kb on + strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.033 kb on - strand, within EX28DRAFT_0313 at 274.147 kb on + strand at 274.171 kb on + strand at 274.171 kb on + strand at 274.172 kb on - strand at 274.180 kb on + strand at 274.181 kb on - strand at 274.240 kb on - strand at 274.291 kb on - strand at 274.291 kb on - strand at 274.525 kb on - strand at 274.670 kb on - strand, within EX28DRAFT_0314 at 274.801 kb on + strand, within EX28DRAFT_0314 at 274.802 kb on - strand, within EX28DRAFT_0314 at 274.802 kb on - strand, within EX28DRAFT_0314 at 274.806 kb on + strand, within EX28DRAFT_0314 at 274.905 kb on + strand, within EX28DRAFT_0314 at 275.089 kb on + strand at 275.089 kb on + strand at 275.090 kb on - strand at 275.104 kb on - strand, within EX28DRAFT_0315 at 275.153 kb on + strand, within EX28DRAFT_0315
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 272,603 - EX28DRAFT_0312 0.87 -0.5 272,868 - -0.9 272,868 - -0.7 272,871 - -0.7 273,212 + EX28DRAFT_0313 0.21 -1.8 273,212 + EX28DRAFT_0313 0.21 -3.1 273,284 + EX28DRAFT_0313 0.27 -2.1 273,285 - EX28DRAFT_0313 0.27 -1.5 273,299 + EX28DRAFT_0313 0.28 -2.9 273,481 - EX28DRAFT_0313 0.43 -0.5 273,599 + EX28DRAFT_0313 0.53 -1.6 273,599 + EX28DRAFT_0313 0.53 -1.7 273,600 - EX28DRAFT_0313 0.53 -0.9 273,678 - EX28DRAFT_0313 0.60 -3.5 273,678 - EX28DRAFT_0313 0.60 -1.5 273,954 + EX28DRAFT_0313 0.83 -1.2 274,033 - EX28DRAFT_0313 0.89 -0.1 274,033 - EX28DRAFT_0313 0.89 -0.9 274,033 - EX28DRAFT_0313 0.89 -0.2 274,147 + -0.3 274,171 + -2.0 274,171 + +0.5 274,172 - -0.6 274,180 + -1.9 274,181 - -0.0 274,240 - +0.9 274,291 - +0.3 274,291 - +1.1 274,525 - -0.3 274,670 - EX28DRAFT_0314 0.38 -0.1 274,801 + EX28DRAFT_0314 0.66 -0.6 274,802 - EX28DRAFT_0314 0.66 +0.1 274,802 - EX28DRAFT_0314 0.66 -1.2 274,806 + EX28DRAFT_0314 0.67 +0.3 274,905 + EX28DRAFT_0314 0.89 +0.1 275,089 + +0.4 275,089 + +0.7 275,090 - +0.3 275,104 - EX28DRAFT_0315 0.12 +0.0 275,153 + EX28DRAFT_0315 0.21 -0.8
Or see this region's nucleotide sequence