Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS01230 and ABIE41_RS01235 are separated by 500 nucleotides ABIE41_RS01235 and ABIE41_RS01240 are separated by 75 nucleotides ABIE41_RS01240 and ABIE41_RS01245 overlap by 11 nucleotides
ABIE41_RS01230: ABIE41_RS01230 - alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase, at 245,126 to 248,056
_RS01230
ABIE41_RS01235: ABIE41_RS01235 - hypothetical protein, at 248,557 to 248,826
_RS01235
ABIE41_RS01240: ABIE41_RS01240 - DNA-3-methyladenine glycosylase, at 248,902 to 249,561
_RS01240
ABIE41_RS01245: ABIE41_RS01245 - CinA family protein, at 249,551 to 250,111
_RS01245
Position (kb) 248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 247.825 kb on - strand at 248.173 kb on - strand at 248.277 kb on + strand at 248.277 kb on + strand at 248.277 kb on + strand at 248.278 kb on - strand at 248.278 kb on - strand at 248.278 kb on - strand at 248.278 kb on - strand at 248.278 kb on - strand at 248.907 kb on + strand at 249.064 kb on + strand, within ABIE41_RS01240 at 249.064 kb on + strand, within ABIE41_RS01240 at 249.065 kb on - strand, within ABIE41_RS01240 at 249.065 kb on - strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.148 kb on + strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.149 kb on - strand, within ABIE41_RS01240 at 249.163 kb on + strand, within ABIE41_RS01240 at 249.163 kb on + strand, within ABIE41_RS01240 at 249.163 kb on + strand, within ABIE41_RS01240 at 249.163 kb on + strand, within ABIE41_RS01240 at 249.164 kb on - strand, within ABIE41_RS01240 at 249.164 kb on - strand, within ABIE41_RS01240 at 249.649 kb on + strand, within ABIE41_RS01245 at 249.649 kb on + strand, within ABIE41_RS01245 at 249.649 kb on + strand, within ABIE41_RS01245 at 249.650 kb on - strand, within ABIE41_RS01245 at 249.740 kb on + strand, within ABIE41_RS01245 at 249.740 kb on + strand, within ABIE41_RS01245 at 249.740 kb on + strand, within ABIE41_RS01245 at 249.740 kb on + strand, within ABIE41_RS01245 at 249.740 kb on + strand, within ABIE41_RS01245 at 249.741 kb on - strand, within ABIE41_RS01245 at 249.741 kb on - strand, within ABIE41_RS01245
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 247,825 - +1.6 248,173 - -0.5 248,277 + +0.3 248,277 + +0.5 248,277 + -1.5 248,278 - +0.4 248,278 - -0.0 248,278 - -0.6 248,278 - +0.2 248,278 - -2.1 248,907 + -0.8 249,064 + ABIE41_RS01240 0.25 -0.9 249,064 + ABIE41_RS01240 0.25 -0.5 249,065 - ABIE41_RS01240 0.25 -0.0 249,065 - ABIE41_RS01240 0.25 +0.3 249,148 + ABIE41_RS01240 0.37 +0.7 249,148 + ABIE41_RS01240 0.37 -2.4 249,148 + ABIE41_RS01240 0.37 +0.1 249,148 + ABIE41_RS01240 0.37 +0.2 249,148 + ABIE41_RS01240 0.37 -1.3 249,148 + ABIE41_RS01240 0.37 -1.8 249,148 + ABIE41_RS01240 0.37 -0.2 249,148 + ABIE41_RS01240 0.37 +0.2 249,148 + ABIE41_RS01240 0.37 -0.5 249,148 + ABIE41_RS01240 0.37 +0.1 249,148 + ABIE41_RS01240 0.37 +1.9 249,149 - ABIE41_RS01240 0.37 -1.5 249,149 - ABIE41_RS01240 0.37 +1.7 249,149 - ABIE41_RS01240 0.37 -0.3 249,149 - ABIE41_RS01240 0.37 +0.5 249,149 - ABIE41_RS01240 0.37 -0.7 249,149 - ABIE41_RS01240 0.37 -3.3 249,149 - ABIE41_RS01240 0.37 -0.2 249,149 - ABIE41_RS01240 0.37 -1.3 249,149 - ABIE41_RS01240 0.37 +1.0 249,163 + ABIE41_RS01240 0.40 -0.4 249,163 + ABIE41_RS01240 0.40 -0.4 249,163 + ABIE41_RS01240 0.40 -1.2 249,163 + ABIE41_RS01240 0.40 +0.8 249,164 - ABIE41_RS01240 0.40 +0.2 249,164 - ABIE41_RS01240 0.40 -0.0 249,649 + ABIE41_RS01245 0.17 +0.7 249,649 + ABIE41_RS01245 0.17 -0.8 249,649 + ABIE41_RS01245 0.17 +1.9 249,650 - ABIE41_RS01245 0.18 -0.1 249,740 + ABIE41_RS01245 0.34 -0.4 249,740 + ABIE41_RS01245 0.34 -2.7 249,740 + ABIE41_RS01245 0.34 +0.6 249,740 + ABIE41_RS01245 0.34 -1.5 249,740 + ABIE41_RS01245 0.34 +0.0 249,741 - ABIE41_RS01245 0.34 -1.9 249,741 - ABIE41_RS01245 0.34 +0.4
Or see this region's nucleotide sequence