Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_1849

Experiment: Cobalt chloride 0.16 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_1846 and PfGW456L13_1847 are separated by 136 nucleotidesPfGW456L13_1847 and PfGW456L13_1848 overlap by 4 nucleotidesPfGW456L13_1848 and PfGW456L13_1849 overlap by 15 nucleotidesPfGW456L13_1849 and PfGW456L13_1850 are separated by 129 nucleotidesPfGW456L13_1850 and PfGW456L13_1851 are separated by 65 nucleotides PfGW456L13_1846 - FIG00955846: hypothetical protein, at 2,035,328 to 2,035,663 _1846 PfGW456L13_1847 - Glycerate kinase (EC 2.7.1.31), at 2,035,800 to 2,036,021 _1847 PfGW456L13_1848 - hypothetical protein, at 2,036,018 to 2,036,155 _1848 PfGW456L13_1849 - Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23), at 2,036,141 to 2,037,916 _1849 PfGW456L13_1850 - FIG000859: hypothetical protein YebC, at 2,038,046 to 2,038,792 _1850 PfGW456L13_1851 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 2,038,858 to 2,039,475 _1851 Position (kb) 2036 2037 2038Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2035.176 kb on + strandat 2035.177 kb on - strandat 2035.177 kb on - strandat 2035.213 kb on + strandat 2035.213 kb on + strandat 2035.214 kb on - strandat 2035.218 kb on + strandat 2035.218 kb on + strandat 2035.219 kb on - strandat 2035.219 kb on - strandat 2035.219 kb on - strandat 2035.219 kb on - strandat 2035.232 kb on + strandat 2035.232 kb on + strandat 2035.232 kb on + strandat 2035.232 kb on + strandat 2035.232 kb on + strandat 2035.233 kb on - strandat 2035.233 kb on - strandat 2035.233 kb on - strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.257 kb on + strandat 2035.258 kb on - strandat 2035.258 kb on - strandat 2035.258 kb on - strandat 2035.258 kb on - strandat 2035.258 kb on - strandat 2035.269 kb on + strandat 2035.269 kb on + strandat 2035.270 kb on - strandat 2035.270 kb on - strandat 2035.270 kb on - strandat 2035.271 kb on + strandat 2035.271 kb on + strandat 2035.272 kb on - strandat 2035.293 kb on + strandat 2035.334 kb on + strandat 2035.335 kb on - strandat 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.377 kb on + strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.378 kb on - strand, within PfGW456L13_1846at 2035.484 kb on + strand, within PfGW456L13_1846at 2035.484 kb on + strand, within PfGW456L13_1846at 2035.485 kb on - strand, within PfGW456L13_1846at 2035.493 kb on - strand, within PfGW456L13_1846at 2035.661 kb on + strandat 2035.705 kb on + strandat 2035.705 kb on + strandat 2035.705 kb on + strandat 2035.705 kb on + strandat 2035.726 kb on + strandat 2035.726 kb on + strandat 2035.733 kb on + strandat 2035.733 kb on + strandat 2035.733 kb on + strandat 2035.735 kb on + strandat 2035.735 kb on + strandat 2035.735 kb on + strandat 2035.735 kb on + strandat 2035.735 kb on + strandat 2035.735 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.809 kb on + strandat 2035.910 kb on + strand, within PfGW456L13_1847at 2035.959 kb on + strand, within PfGW456L13_1847at 2036.019 kb on + strandat 2036.095 kb on + strand, within PfGW456L13_1848at 2036.095 kb on + strand, within PfGW456L13_1848at 2036.095 kb on + strand, within PfGW456L13_1848at 2037.914 kb on + strandat 2037.958 kb on + strandat 2037.958 kb on + strandat 2037.958 kb on + strandat 2037.960 kb on + strandat 2037.960 kb on + strandat 2037.960 kb on + strandat 2037.960 kb on + strandat 2037.961 kb on - strandat 2037.961 kb on - strandat 2038.045 kb on + strandat 2038.045 kb on + strandat 2038.046 kb on - strandat 2038.304 kb on + strand, within PfGW456L13_1850at 2038.304 kb on + strand, within PfGW456L13_1850at 2038.304 kb on + strand, within PfGW456L13_1850at 2038.304 kb on + strand, within PfGW456L13_1850at 2038.304 kb on + strand, within PfGW456L13_1850at 2038.304 kb on + strand, within PfGW456L13_1850at 2038.305 kb on - strand, within PfGW456L13_1850at 2038.314 kb on - strand, within PfGW456L13_1850at 2038.364 kb on - strand, within PfGW456L13_1850at 2038.439 kb on + strand, within PfGW456L13_1850at 2038.439 kb on + strand, within PfGW456L13_1850at 2038.439 kb on + strand, within PfGW456L13_1850at 2038.439 kb on + strand, within PfGW456L13_1850at 2038.439 kb on + strand, within PfGW456L13_1850at 2038.440 kb on - strand, within PfGW456L13_1850at 2038.586 kb on + strand, within PfGW456L13_1850at 2038.586 kb on + strand, within PfGW456L13_1850at 2038.586 kb on + strand, within PfGW456L13_1850at 2038.587 kb on - strand, within PfGW456L13_1850at 2038.587 kb on - strand, within PfGW456L13_1850at 2038.587 kb on - strand, within PfGW456L13_1850at 2038.746 kb on - strandat 2038.807 kb on + strandat 2038.870 kb on + strandat 2038.871 kb on - strandat 2038.899 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM
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2,035,176 + +0.5
2,035,177 - -0.7
2,035,177 - -0.8
2,035,213 + +0.4
2,035,213 + -0.2
2,035,214 - -1.1
2,035,218 + +0.6
2,035,218 + -0.3
2,035,219 - -2.2
2,035,219 - +0.2
2,035,219 - +0.6
2,035,219 - -0.2
2,035,232 + -0.4
2,035,232 + -0.3
2,035,232 + -0.0
2,035,232 + -0.1
2,035,232 + -0.9
2,035,233 - -0.4
2,035,233 - -0.6
2,035,233 - +0.5
2,035,257 + +1.2
2,035,257 + +0.6
2,035,257 + +0.2
2,035,257 + +0.6
2,035,257 + -1.5
2,035,257 + +0.8
2,035,257 + +0.2
2,035,258 - -0.3
2,035,258 - -1.1
2,035,258 - +0.2
2,035,258 - +0.3
2,035,258 - -2.1
2,035,269 + +0.7
2,035,269 + -0.9
2,035,270 - +0.2
2,035,270 - -0.4
2,035,270 - -0.9
2,035,271 + -0.1
2,035,271 + -0.2
2,035,272 - -1.1
2,035,293 + +0.3
2,035,334 + +0.6
2,035,335 - -0.8
2,035,377 + PfGW456L13_1846 0.15 +0.1
2,035,377 + PfGW456L13_1846 0.15 -1.2
2,035,377 + PfGW456L13_1846 0.15 +0.6
2,035,377 + PfGW456L13_1846 0.15 +0.3
2,035,377 + PfGW456L13_1846 0.15 +0.1
2,035,377 + PfGW456L13_1846 0.15 +1.4
2,035,377 + PfGW456L13_1846 0.15 +1.3
2,035,377 + PfGW456L13_1846 0.15 +0.3
2,035,377 + PfGW456L13_1846 0.15 +0.5
2,035,377 + PfGW456L13_1846 0.15 -0.5
2,035,377 + PfGW456L13_1846 0.15 +0.8
2,035,378 - PfGW456L13_1846 0.15 -1.9
2,035,378 - PfGW456L13_1846 0.15 +0.9
2,035,378 - PfGW456L13_1846 0.15 +0.4
2,035,378 - PfGW456L13_1846 0.15 -0.1
2,035,378 - PfGW456L13_1846 0.15 +0.5
2,035,378 - PfGW456L13_1846 0.15 +0.8
2,035,378 - PfGW456L13_1846 0.15 -1.6
2,035,378 - PfGW456L13_1846 0.15 +1.6
2,035,378 - PfGW456L13_1846 0.15 -0.6
2,035,484 + PfGW456L13_1846 0.46 -1.0
2,035,484 + PfGW456L13_1846 0.46 -0.8
2,035,485 - PfGW456L13_1846 0.47 -2.1
2,035,493 - PfGW456L13_1846 0.49 -0.3
2,035,661 + +0.8
2,035,705 + -0.3
2,035,705 + +0.4
2,035,705 + -1.6
2,035,705 + +0.2
2,035,726 + +0.7
2,035,726 + -0.6
2,035,733 + -2.4
2,035,733 + -0.6
2,035,733 + -0.1
2,035,735 + +0.8
2,035,735 + +0.5
2,035,735 + -0.6
2,035,735 + +0.2
2,035,735 + -0.5
2,035,735 + -0.8
2,035,809 + +0.6
2,035,809 + +0.2
2,035,809 + +0.0
2,035,809 + +0.1
2,035,809 + -0.1
2,035,809 + +0.4
2,035,809 + +0.3
2,035,809 + -0.6
2,035,910 + PfGW456L13_1847 0.50 -0.6
2,035,959 + PfGW456L13_1847 0.72 +0.1
2,036,019 + +0.4
2,036,095 + PfGW456L13_1848 0.56 +1.3
2,036,095 + PfGW456L13_1848 0.56 +2.1
2,036,095 + PfGW456L13_1848 0.56 +1.4
2,037,914 + +0.8
2,037,958 + +0.4
2,037,958 + -0.9
2,037,958 + +0.9
2,037,960 + -0.9
2,037,960 + -0.0
2,037,960 + +0.1
2,037,960 + +0.9
2,037,961 - -1.6
2,037,961 - -0.2
2,038,045 + -1.9
2,038,045 + -2.2
2,038,046 - +0.6
2,038,304 + PfGW456L13_1850 0.35 -2.2
2,038,304 + PfGW456L13_1850 0.35 -2.4
2,038,304 + PfGW456L13_1850 0.35 -3.0
2,038,304 + PfGW456L13_1850 0.35 -2.9
2,038,304 + PfGW456L13_1850 0.35 -2.4
2,038,304 + PfGW456L13_1850 0.35 -4.2
2,038,305 - PfGW456L13_1850 0.35 -1.5
2,038,314 - PfGW456L13_1850 0.36 -1.8
2,038,364 - PfGW456L13_1850 0.43 -0.7
2,038,439 + PfGW456L13_1850 0.53 -2.7
2,038,439 + PfGW456L13_1850 0.53 -1.1
2,038,439 + PfGW456L13_1850 0.53 -2.7
2,038,439 + PfGW456L13_1850 0.53 -2.3
2,038,439 + PfGW456L13_1850 0.53 -1.2
2,038,440 - PfGW456L13_1850 0.53 -0.9
2,038,586 + PfGW456L13_1850 0.72 -1.8
2,038,586 + PfGW456L13_1850 0.72 -1.0
2,038,586 + PfGW456L13_1850 0.72 -1.5
2,038,587 - PfGW456L13_1850 0.72 -0.1
2,038,587 - PfGW456L13_1850 0.72 -3.0
2,038,587 - PfGW456L13_1850 0.72 -1.1
2,038,746 - -1.2
2,038,807 + +0.1
2,038,870 + +0.5
2,038,871 - -1.5
2,038,899 - +0.3

Or see this region's nucleotide sequence