Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS23540 and PFLU_RS23545 overlap by 4 nucleotides PFLU_RS23545 and PFLU_RS23550 are separated by 32 nucleotides PFLU_RS23550 and PFLU_RS23555 are separated by 42 nucleotides PFLU_RS23555 and PFLU_RS23560 are separated by 147 nucleotides
PFLU_RS23540: PFLU_RS23540 - RNA polymerase sigma factor, at 5,284,599 to 5,285,822
_RS23540
PFLU_RS23545: PFLU_RS23545 - SRPBCC family protein, at 5,285,819 to 5,286,298
_RS23545
PFLU_RS23550: PFLU_RS23550 - YciI family protein, at 5,286,331 to 5,286,675
_RS23550
PFLU_RS23555: PFLU_RS23555 - DUF4440 domain-containing protein, at 5,286,718 to 5,287,137
_RS23555
PFLU_RS23560: PFLU_RS23560 - GNAT family N-acetyltransferase, at 5,287,285 to 5,287,779
_RS23560
Position (kb) 5285
5286
5287 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5284.894 kb on + strand, within PFLU_RS23540 at 5284.895 kb on - strand, within PFLU_RS23540 at 5285.202 kb on - strand, within PFLU_RS23540 at 5285.202 kb on - strand, within PFLU_RS23540 at 5285.254 kb on + strand, within PFLU_RS23540 at 5285.275 kb on + strand, within PFLU_RS23540 at 5285.275 kb on + strand, within PFLU_RS23540 at 5285.275 kb on + strand, within PFLU_RS23540 at 5285.276 kb on - strand, within PFLU_RS23540 at 5285.378 kb on + strand, within PFLU_RS23540 at 5285.378 kb on + strand, within PFLU_RS23540 at 5285.378 kb on + strand, within PFLU_RS23540 at 5285.378 kb on + strand, within PFLU_RS23540 at 5285.379 kb on - strand, within PFLU_RS23540 at 5285.379 kb on - strand, within PFLU_RS23540 at 5285.379 kb on - strand, within PFLU_RS23540 at 5285.379 kb on - strand, within PFLU_RS23540 at 5285.379 kb on - strand, within PFLU_RS23540 at 5285.797 kb on + strand at 5285.946 kb on + strand, within PFLU_RS23545 at 5285.946 kb on + strand, within PFLU_RS23545 at 5285.946 kb on + strand, within PFLU_RS23545 at 5285.947 kb on - strand, within PFLU_RS23545 at 5285.947 kb on - strand, within PFLU_RS23545 at 5285.947 kb on - strand, within PFLU_RS23545 at 5285.947 kb on - strand, within PFLU_RS23545 at 5286.040 kb on + strand, within PFLU_RS23545 at 5286.094 kb on - strand, within PFLU_RS23545 at 5286.292 kb on + strand at 5286.292 kb on + strand at 5286.293 kb on - strand at 5286.303 kb on + strand at 5286.303 kb on + strand at 5286.319 kb on + strand at 5286.319 kb on + strand at 5286.319 kb on + strand at 5286.319 kb on + strand at 5286.319 kb on + strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.320 kb on - strand at 5286.321 kb on - strand at 5286.440 kb on + strand, within PFLU_RS23550 at 5286.440 kb on + strand, within PFLU_RS23550 at 5286.440 kb on + strand, within PFLU_RS23550 at 5286.440 kb on + strand, within PFLU_RS23550 at 5286.441 kb on - strand, within PFLU_RS23550 at 5286.441 kb on - strand, within PFLU_RS23550 at 5286.441 kb on - strand, within PFLU_RS23550 at 5286.441 kb on - strand, within PFLU_RS23550 at 5286.441 kb on - strand, within PFLU_RS23550 at 5286.590 kb on + strand, within PFLU_RS23550 at 5286.590 kb on + strand, within PFLU_RS23550 at 5286.590 kb on + strand, within PFLU_RS23550 at 5286.590 kb on + strand, within PFLU_RS23550 at 5286.590 kb on + strand, within PFLU_RS23550 at 5286.591 kb on - strand, within PFLU_RS23550 at 5286.591 kb on - strand, within PFLU_RS23550 at 5286.653 kb on + strand at 5286.654 kb on - strand at 5286.654 kb on - strand at 5286.668 kb on + strand at 5286.668 kb on + strand at 5286.668 kb on + strand at 5286.668 kb on + strand at 5286.668 kb on + strand at 5286.669 kb on - strand at 5286.669 kb on - strand at 5286.669 kb on - strand at 5286.696 kb on + strand at 5286.697 kb on - strand at 5286.703 kb on + strand at 5286.703 kb on + strand at 5286.703 kb on + strand at 5286.703 kb on + strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.704 kb on - strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.705 kb on + strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.706 kb on - strand at 5286.804 kb on + strand, within PFLU_RS23555 at 5286.804 kb on + strand, within PFLU_RS23555 at 5286.846 kb on + strand, within PFLU_RS23555 at 5286.846 kb on + strand, within PFLU_RS23555 at 5286.846 kb on + strand, within PFLU_RS23555 at 5286.847 kb on - strand, within PFLU_RS23555 at 5286.847 kb on - strand, within PFLU_RS23555 at 5286.847 kb on - strand, within PFLU_RS23555 at 5286.992 kb on + strand, within PFLU_RS23555 at 5287.055 kb on + strand, within PFLU_RS23555 at 5287.055 kb on + strand, within PFLU_RS23555 at 5287.055 kb on + strand, within PFLU_RS23555 at 5287.056 kb on - strand, within PFLU_RS23555 at 5287.056 kb on - strand, within PFLU_RS23555 at 5287.143 kb on + strand at 5287.143 kb on + strand at 5287.144 kb on - strand at 5287.144 kb on - strand at 5287.170 kb on + strand at 5287.170 kb on + strand at 5287.171 kb on - strand at 5287.171 kb on - strand at 5287.171 kb on - strand at 5287.171 kb on - strand at 5287.171 kb on - strand at 5287.171 kb on - strand at 5287.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 5,284,894 + PFLU_RS23540 0.24 -0.7 5,284,895 - PFLU_RS23540 0.24 +1.2 5,285,202 - PFLU_RS23540 0.49 +1.2 5,285,202 - PFLU_RS23540 0.49 -0.9 5,285,254 + PFLU_RS23540 0.54 +1.4 5,285,275 + PFLU_RS23540 0.55 -0.3 5,285,275 + PFLU_RS23540 0.55 +0.9 5,285,275 + PFLU_RS23540 0.55 -1.3 5,285,276 - PFLU_RS23540 0.55 -0.2 5,285,378 + PFLU_RS23540 0.64 -0.3 5,285,378 + PFLU_RS23540 0.64 -1.9 5,285,378 + PFLU_RS23540 0.64 +0.1 5,285,378 + PFLU_RS23540 0.64 +0.4 5,285,379 - PFLU_RS23540 0.64 -0.4 5,285,379 - PFLU_RS23540 0.64 -0.5 5,285,379 - PFLU_RS23540 0.64 +0.6 5,285,379 - PFLU_RS23540 0.64 +0.4 5,285,379 - PFLU_RS23540 0.64 +0.5 5,285,797 + +0.4 5,285,946 + PFLU_RS23545 0.26 -0.3 5,285,946 + PFLU_RS23545 0.26 -0.3 5,285,946 + PFLU_RS23545 0.26 -0.1 5,285,947 - PFLU_RS23545 0.27 -1.0 5,285,947 - PFLU_RS23545 0.27 -0.2 5,285,947 - PFLU_RS23545 0.27 -0.4 5,285,947 - PFLU_RS23545 0.27 -0.1 5,286,040 + PFLU_RS23545 0.46 -1.3 5,286,094 - PFLU_RS23545 0.57 -0.1 5,286,292 + +0.1 5,286,292 + +0.0 5,286,293 - +0.3 5,286,303 + +0.2 5,286,303 + -2.7 5,286,319 + -0.9 5,286,319 + +0.9 5,286,319 + +0.1 5,286,319 + +1.0 5,286,319 + +0.6 5,286,320 - -0.1 5,286,320 - -0.8 5,286,320 - +1.8 5,286,320 - +0.0 5,286,320 - -2.3 5,286,320 - +0.3 5,286,320 - +0.3 5,286,320 - +0.3 5,286,321 - -0.7 5,286,440 + PFLU_RS23550 0.32 -0.1 5,286,440 + PFLU_RS23550 0.32 -1.9 5,286,440 + PFLU_RS23550 0.32 -1.0 5,286,440 + PFLU_RS23550 0.32 -0.5 5,286,441 - PFLU_RS23550 0.32 +0.0 5,286,441 - PFLU_RS23550 0.32 +0.1 5,286,441 - PFLU_RS23550 0.32 -0.5 5,286,441 - PFLU_RS23550 0.32 +0.5 5,286,441 - PFLU_RS23550 0.32 +0.7 5,286,590 + PFLU_RS23550 0.75 -0.3 5,286,590 + PFLU_RS23550 0.75 -1.3 5,286,590 + PFLU_RS23550 0.75 +0.5 5,286,590 + PFLU_RS23550 0.75 -0.4 5,286,590 + PFLU_RS23550 0.75 +0.7 5,286,591 - PFLU_RS23550 0.75 -1.1 5,286,591 - PFLU_RS23550 0.75 +0.2 5,286,653 + +0.9 5,286,654 - +0.2 5,286,654 - -0.9 5,286,668 + +0.3 5,286,668 + -0.5 5,286,668 + -1.2 5,286,668 + -0.4 5,286,668 + +0.1 5,286,669 - -1.7 5,286,669 - -0.7 5,286,669 - +0.2 5,286,696 + +0.1 5,286,697 - -0.3 5,286,703 + -2.0 5,286,703 + -2.1 5,286,703 + -0.9 5,286,703 + -0.0 5,286,704 - +0.5 5,286,704 - -2.1 5,286,704 - +0.3 5,286,704 - +0.5 5,286,704 - -0.4 5,286,704 - +0.2 5,286,704 - -1.1 5,286,705 + +0.3 5,286,705 + +0.2 5,286,705 + +0.8 5,286,705 + +0.2 5,286,705 + +1.0 5,286,705 + +0.2 5,286,705 + -0.5 5,286,705 + +0.2 5,286,705 + -2.7 5,286,705 + +0.6 5,286,705 + +0.4 5,286,705 + +0.0 5,286,705 + +0.0 5,286,705 + -0.8 5,286,705 + +0.1 5,286,705 + +0.2 5,286,705 + -0.2 5,286,705 + +1.2 5,286,706 - -0.3 5,286,706 - -2.3 5,286,706 - +0.8 5,286,706 - -1.2 5,286,706 - +0.4 5,286,706 - +0.3 5,286,706 - +1.3 5,286,706 - +1.1 5,286,706 - +1.4 5,286,706 - +0.5 5,286,706 - -0.4 5,286,804 + PFLU_RS23555 0.20 +0.6 5,286,804 + PFLU_RS23555 0.20 -2.2 5,286,846 + PFLU_RS23555 0.30 -1.7 5,286,846 + PFLU_RS23555 0.30 -0.6 5,286,846 + PFLU_RS23555 0.30 -1.3 5,286,847 - PFLU_RS23555 0.31 -1.1 5,286,847 - PFLU_RS23555 0.31 +0.0 5,286,847 - PFLU_RS23555 0.31 -0.1 5,286,992 + PFLU_RS23555 0.65 +0.1 5,287,055 + PFLU_RS23555 0.80 +0.8 5,287,055 + PFLU_RS23555 0.80 +0.8 5,287,055 + PFLU_RS23555 0.80 +1.0 5,287,056 - PFLU_RS23555 0.80 -0.2 5,287,056 - PFLU_RS23555 0.80 +0.6 5,287,143 + -1.1 5,287,143 + +1.2 5,287,144 - -0.4 5,287,144 - +0.1 5,287,170 + +0.9 5,287,170 + +0.1 5,287,171 - -1.5 5,287,171 - -0.5 5,287,171 - +0.4 5,287,171 - -0.9 5,287,171 - +0.0 5,287,171 - -0.8 5,287,171 - +0.4
Or see this region's nucleotide sequence