Experiment: succinate (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS22990 and PFLU_RS22995 overlap by 4 nucleotides PFLU_RS22995 and PFLU_RS23000 are separated by 70 nucleotides PFLU_RS23000 and PFLU_RS23005 are separated by 16 nucleotides PFLU_RS23005 and PFLU_RS23010 are separated by 138 nucleotides
PFLU_RS22990: PFLU_RS22990 - ADP-ribosylglycohydrolase, at 5,169,286 to 5,170,287
_RS22990
PFLU_RS22995: PFLU_RS22995 - GntR family transcriptional regulator, at 5,170,284 to 5,170,997
_RS22995
PFLU_RS23000: PFLU_RS23000 - DUF1285 domain-containing protein, at 5,171,068 to 5,171,628
_RS23000
PFLU_RS23005: PFLU_RS23005 - DUF4823 domain-containing protein, at 5,171,645 to 5,172,250
_RS23005
PFLU_RS23010: PFLU_RS23010 - radical SAM protein, at 5,172,389 to 5,173,357
_RS23010
Position (kb) 5171
5172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5170.137 kb on + strand, within PFLU_RS22990 at 5170.253 kb on + strand at 5170.254 kb on - strand at 5170.348 kb on + strand at 5170.349 kb on - strand at 5170.349 kb on - strand at 5170.349 kb on - strand at 5170.349 kb on - strand at 5170.349 kb on - strand at 5170.349 kb on - strand at 5170.375 kb on + strand, within PFLU_RS22995 at 5170.375 kb on + strand, within PFLU_RS22995 at 5170.375 kb on + strand, within PFLU_RS22995 at 5170.375 kb on + strand, within PFLU_RS22995 at 5170.376 kb on - strand, within PFLU_RS22995 at 5170.376 kb on - strand, within PFLU_RS22995 at 5170.376 kb on - strand, within PFLU_RS22995 at 5170.376 kb on - strand, within PFLU_RS22995 at 5170.382 kb on + strand, within PFLU_RS22995 at 5170.382 kb on + strand, within PFLU_RS22995 at 5170.489 kb on + strand, within PFLU_RS22995 at 5170.816 kb on + strand, within PFLU_RS22995 at 5170.816 kb on + strand, within PFLU_RS22995 at 5170.816 kb on + strand, within PFLU_RS22995 at 5170.816 kb on + strand, within PFLU_RS22995 at 5170.817 kb on - strand, within PFLU_RS22995 at 5170.817 kb on - strand, within PFLU_RS22995 at 5170.817 kb on - strand, within PFLU_RS22995 at 5170.817 kb on - strand, within PFLU_RS22995 at 5171.041 kb on + strand at 5171.041 kb on + strand at 5171.168 kb on + strand, within PFLU_RS23000 at 5171.168 kb on + strand, within PFLU_RS23000 at 5171.169 kb on - strand, within PFLU_RS23000 at 5171.215 kb on + strand, within PFLU_RS23000 at 5171.215 kb on + strand, within PFLU_RS23000 at 5171.215 kb on + strand, within PFLU_RS23000 at 5171.216 kb on - strand, within PFLU_RS23000 at 5171.216 kb on - strand, within PFLU_RS23000 at 5171.216 kb on - strand, within PFLU_RS23000 at 5171.219 kb on + strand, within PFLU_RS23000 at 5171.219 kb on + strand, within PFLU_RS23000 at 5171.220 kb on - strand, within PFLU_RS23000 at 5171.220 kb on - strand, within PFLU_RS23000 at 5171.220 kb on - strand, within PFLU_RS23000 at 5171.341 kb on + strand, within PFLU_RS23000 at 5171.341 kb on + strand, within PFLU_RS23000 at 5171.341 kb on + strand, within PFLU_RS23000 at 5171.417 kb on + strand, within PFLU_RS23000 at 5171.417 kb on + strand, within PFLU_RS23000 at 5171.417 kb on + strand, within PFLU_RS23000 at 5171.417 kb on + strand, within PFLU_RS23000 at 5171.470 kb on - strand, within PFLU_RS23000 at 5171.493 kb on - strand, within PFLU_RS23000 at 5171.647 kb on - strand at 5171.647 kb on - strand at 5171.647 kb on - strand at 5171.647 kb on - strand at 5171.680 kb on - strand at 5171.735 kb on + strand, within PFLU_RS23005 at 5171.755 kb on + strand, within PFLU_RS23005 at 5171.755 kb on + strand, within PFLU_RS23005 at 5171.755 kb on + strand, within PFLU_RS23005 at 5171.755 kb on + strand, within PFLU_RS23005 at 5171.756 kb on - strand, within PFLU_RS23005 at 5171.756 kb on - strand, within PFLU_RS23005 at 5171.764 kb on + strand, within PFLU_RS23005 at 5171.765 kb on - strand, within PFLU_RS23005 at 5171.765 kb on - strand, within PFLU_RS23005 at 5171.765 kb on - strand, within PFLU_RS23005 at 5171.765 kb on - strand, within PFLU_RS23005 at 5171.775 kb on + strand, within PFLU_RS23005 at 5171.776 kb on - strand, within PFLU_RS23005 at 5171.776 kb on - strand, within PFLU_RS23005 at 5171.776 kb on - strand, within PFLU_RS23005 at 5171.904 kb on + strand, within PFLU_RS23005 at 5171.905 kb on - strand, within PFLU_RS23005 at 5171.905 kb on - strand, within PFLU_RS23005 at 5171.913 kb on + strand, within PFLU_RS23005 at 5171.913 kb on + strand, within PFLU_RS23005 at 5171.995 kb on - strand, within PFLU_RS23005 at 5171.995 kb on - strand, within PFLU_RS23005 at 5172.082 kb on + strand, within PFLU_RS23005 at 5172.082 kb on + strand, within PFLU_RS23005 at 5172.083 kb on - strand, within PFLU_RS23005 at 5172.083 kb on - strand, within PFLU_RS23005 at 5172.088 kb on + strand, within PFLU_RS23005 at 5172.244 kb on + strand at 5172.244 kb on + strand at 5172.244 kb on + strand at 5172.244 kb on + strand at 5172.245 kb on - strand at 5172.245 kb on - strand at 5172.245 kb on - strand at 5172.245 kb on - strand at 5172.245 kb on - strand at 5172.245 kb on - strand at 5172.253 kb on + strand at 5172.253 kb on + strand at 5172.253 kb on + strand at 5172.253 kb on + strand at 5172.253 kb on + strand at 5172.253 kb on + strand at 5172.254 kb on - strand at 5172.254 kb on - strand at 5172.254 kb on - strand at 5172.254 kb on - strand at 5172.321 kb on + strand at 5172.329 kb on + strand at 5172.329 kb on + strand at 5172.329 kb on + strand at 5172.329 kb on + strand at 5172.329 kb on + strand at 5172.329 kb on + strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.330 kb on - strand at 5172.366 kb on + strand at 5172.434 kb on + strand at 5172.434 kb on + strand at 5172.434 kb on + strand at 5172.434 kb on + strand at 5172.435 kb on - strand at 5172.435 kb on - strand at 5172.435 kb on - strand at 5172.525 kb on + strand, within PFLU_RS23010 at 5172.525 kb on + strand, within PFLU_RS23010 at 5172.525 kb on + strand, within PFLU_RS23010 at 5172.525 kb on + strand, within PFLU_RS23010 at 5172.526 kb on - strand, within PFLU_RS23010 at 5172.526 kb on - strand, within PFLU_RS23010 at 5172.526 kb on - strand, within PFLU_RS23010 at 5172.526 kb on - strand, within PFLU_RS23010 at 5172.526 kb on - strand, within PFLU_RS23010 at 5172.596 kb on - strand, within PFLU_RS23010
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C); with MOPS remove 5,170,137 + PFLU_RS22990 0.85 -0.2 5,170,253 + +0.3 5,170,254 - +0.1 5,170,348 + -1.1 5,170,349 - +0.0 5,170,349 - +0.9 5,170,349 - -0.5 5,170,349 - +3.1 5,170,349 - +0.6 5,170,349 - -1.5 5,170,375 + PFLU_RS22995 0.13 -2.8 5,170,375 + PFLU_RS22995 0.13 +0.1 5,170,375 + PFLU_RS22995 0.13 +1.0 5,170,375 + PFLU_RS22995 0.13 +0.6 5,170,376 - PFLU_RS22995 0.13 +0.6 5,170,376 - PFLU_RS22995 0.13 +0.5 5,170,376 - PFLU_RS22995 0.13 -0.0 5,170,376 - PFLU_RS22995 0.13 +0.5 5,170,382 + PFLU_RS22995 0.14 +1.1 5,170,382 + PFLU_RS22995 0.14 +0.4 5,170,489 + PFLU_RS22995 0.29 +0.1 5,170,816 + PFLU_RS22995 0.75 +0.3 5,170,816 + PFLU_RS22995 0.75 -0.1 5,170,816 + PFLU_RS22995 0.75 +1.4 5,170,816 + PFLU_RS22995 0.75 -1.6 5,170,817 - PFLU_RS22995 0.75 -0.6 5,170,817 - PFLU_RS22995 0.75 -0.3 5,170,817 - PFLU_RS22995 0.75 -1.2 5,170,817 - PFLU_RS22995 0.75 +0.0 5,171,041 + -0.5 5,171,041 + +0.3 5,171,168 + PFLU_RS23000 0.18 -0.6 5,171,168 + PFLU_RS23000 0.18 +0.9 5,171,169 - PFLU_RS23000 0.18 -0.1 5,171,215 + PFLU_RS23000 0.26 -0.7 5,171,215 + PFLU_RS23000 0.26 +0.1 5,171,215 + PFLU_RS23000 0.26 +1.3 5,171,216 - PFLU_RS23000 0.26 -1.0 5,171,216 - PFLU_RS23000 0.26 +0.4 5,171,216 - PFLU_RS23000 0.26 -0.9 5,171,219 + PFLU_RS23000 0.27 -0.5 5,171,219 + PFLU_RS23000 0.27 +0.3 5,171,220 - PFLU_RS23000 0.27 -0.0 5,171,220 - PFLU_RS23000 0.27 +0.3 5,171,220 - PFLU_RS23000 0.27 -0.2 5,171,341 + PFLU_RS23000 0.49 +0.4 5,171,341 + PFLU_RS23000 0.49 -1.0 5,171,341 + PFLU_RS23000 0.49 +1.7 5,171,417 + PFLU_RS23000 0.62 +0.8 5,171,417 + PFLU_RS23000 0.62 +1.1 5,171,417 + PFLU_RS23000 0.62 +0.9 5,171,417 + PFLU_RS23000 0.62 +0.9 5,171,470 - PFLU_RS23000 0.72 -0.9 5,171,493 - PFLU_RS23000 0.76 -1.0 5,171,647 - -0.4 5,171,647 - +1.4 5,171,647 - -0.7 5,171,647 - -0.4 5,171,680 - -0.6 5,171,735 + PFLU_RS23005 0.15 -0.1 5,171,755 + PFLU_RS23005 0.18 +0.8 5,171,755 + PFLU_RS23005 0.18 -0.6 5,171,755 + PFLU_RS23005 0.18 +0.7 5,171,755 + PFLU_RS23005 0.18 +0.8 5,171,756 - PFLU_RS23005 0.18 +0.4 5,171,756 - PFLU_RS23005 0.18 -0.4 5,171,764 + PFLU_RS23005 0.20 +1.0 5,171,765 - PFLU_RS23005 0.20 +0.9 5,171,765 - PFLU_RS23005 0.20 +1.1 5,171,765 - PFLU_RS23005 0.20 +0.9 5,171,765 - PFLU_RS23005 0.20 +0.3 5,171,775 + PFLU_RS23005 0.21 +0.6 5,171,776 - PFLU_RS23005 0.22 +0.2 5,171,776 - PFLU_RS23005 0.22 +0.9 5,171,776 - PFLU_RS23005 0.22 -0.1 5,171,904 + PFLU_RS23005 0.43 -0.9 5,171,905 - PFLU_RS23005 0.43 +0.3 5,171,905 - PFLU_RS23005 0.43 +1.1 5,171,913 + PFLU_RS23005 0.44 +0.7 5,171,913 + PFLU_RS23005 0.44 +0.0 5,171,995 - PFLU_RS23005 0.58 +0.4 5,171,995 - PFLU_RS23005 0.58 -1.0 5,172,082 + PFLU_RS23005 0.72 +0.9 5,172,082 + PFLU_RS23005 0.72 +0.4 5,172,083 - PFLU_RS23005 0.72 +1.5 5,172,083 - PFLU_RS23005 0.72 -0.6 5,172,088 + PFLU_RS23005 0.73 +0.1 5,172,244 + +1.2 5,172,244 + +0.5 5,172,244 + -1.0 5,172,244 + +0.5 5,172,245 - +0.9 5,172,245 - -0.6 5,172,245 - +0.7 5,172,245 - +1.0 5,172,245 - -0.6 5,172,245 - -0.3 5,172,253 + -0.8 5,172,253 + +0.9 5,172,253 + +0.4 5,172,253 + -0.1 5,172,253 + +0.3 5,172,253 + +1.4 5,172,254 - -0.6 5,172,254 - -0.1 5,172,254 - +0.0 5,172,254 - -0.1 5,172,321 + +0.3 5,172,329 + -0.7 5,172,329 + +1.0 5,172,329 + -0.1 5,172,329 + +0.4 5,172,329 + -0.4 5,172,329 + -0.8 5,172,330 - +0.8 5,172,330 - +0.3 5,172,330 - -1.1 5,172,330 - +0.9 5,172,330 - -0.2 5,172,330 - +1.1 5,172,330 - +0.7 5,172,330 - +0.9 5,172,330 - +0.7 5,172,330 - -0.1 5,172,366 + +0.0 5,172,434 + +0.4 5,172,434 + +0.5 5,172,434 + +0.1 5,172,434 + -0.1 5,172,435 - -0.2 5,172,435 - -1.7 5,172,435 - +1.5 5,172,525 + PFLU_RS23010 0.14 +0.3 5,172,525 + PFLU_RS23010 0.14 +1.6 5,172,525 + PFLU_RS23010 0.14 +1.5 5,172,525 + PFLU_RS23010 0.14 +0.4 5,172,526 - PFLU_RS23010 0.14 -0.1 5,172,526 - PFLU_RS23010 0.14 -0.3 5,172,526 - PFLU_RS23010 0.14 +0.7 5,172,526 - PFLU_RS23010 0.14 -0.1 5,172,526 - PFLU_RS23010 0.14 +0.1 5,172,596 - PFLU_RS23010 0.21 -0.1
Or see this region's nucleotide sequence